Results 61 to 70 of about 2,999 (176)

Engineering Fluoroacetate Dehalogenase by Growth‐Based Selections on Non‐Natural Organofluorides

Angewandte Chemie International Edition, EarlyView.
We present a high‐throughput selection system to engineer fluoroacetate dehalogenases (FAcDs). By challenging E. coli populations that produce diverse FAcD libraries to grow on non‐natural organofluorides as their sole carbon source, we isolated a panel of FAcD variants with improved activity and altered substrate specificity.
Suzanne C. Jansen, Pauline van Beers, Clemens Mayer   +2 more
wiley   +1 more source

Effect of Moderate Heat on TCE Reductive Dechlorination Rates in Groundwater

Groundwater Monitoring &Remediation, EarlyView.
Abstract Low‐temperature heating (in the mesophilic range of ~15 to 40 °C) of contaminated aquifers offers the prospect of increasing the rates for biotic and abiotic treatment of volatile organic compounds. Thermal In Situ Sustainable Remediation (TISR®) is one of the approaches available to implement low temperature heating.
David L. Freedman, Nicholas Cotter, Craig Divine, Shandra Justicia‐Leone   +3 more
wiley   +1 more source

Identification of abiotic and biotic reductive dechlorination in a chlorinated ethene plume after thermal source remediation by means of isotopic and molecular biology tools [PDF]

, 2016
Thermal tetrachloroethene (PCE) remediation by steam injection in a sandy aquifer led to the release of dissolved organic carbon (DOC) from aquifer sediments resulting in more reduced redox conditions, accelerated PCE biodegradation, and changes in ...
Badin, Alice, Broholm, Mette M, Dennis, Philip, Hunkeler, Daniel, Jacobsen, Carsten S, Palau, Jordi   +5 more
core   +2 more sources

Pentachlorophenol and spent engine oil degradation by Mucor ramosissimus [PDF]

, 2008
Pentachlorophenol (PCP) has been widely used for many years and belongs to the most toxic pollutants. Spent engine oils enter environment every day in many ways. Both of them cause great environmental concern.
Długoński, Jerzy, Szewczyk, Rafał
core   +1 more source

An α/β‐Hydrolase GmABHD6 Controls Seed Oil Content and Yield in Soybean

Plant Biotechnology Journal, EarlyView.
ABSTRACT Seed oil content is a key trait in soybean, yet its genetic basis remains largely unresolved. Here, we identify GmABHD6, an α/β‐hydrolase domain‐containing gene, as the causal gene underlying a major quantitative trait locus (QTL) for seed oil content on chromosome 16.
Kaixin Yu, Limin Hu, Bo Sun, Zehao Chen, Xu Li, Shiyu Zhang, Huilin Tian, Wenjing Zhang, Shimin Ren, Xue Han, Chang Xu, Siming Wei, Mingliang Yang, Fanjiang Kong, Qingshan Chen, Zhaoming Qi   +15 more
wiley   +1 more source

Contrasting Kinetics of Highly Similar Chloroalkane Reductive Dehalogenases

Environmental Science & Technology
ABSTRACTChloroform and trichloroethanes are pervasive groundwater contaminants for which bioremediation has been an effective treatment strategy. Reductive dehalogenase (RDase) enzymes from organohalide-respiring bacteria are essential for their remediation under anaerobic conditions. RDases are responsible for dehalogenating these chlorinated solvents,
Katherine J. Picott, Elizabeth A. Edwards   +1 more
openaire   +2 more sources

Identification of a Chlorobenzene Reductive Dehalogenase in Dehalococcoides sp. Strain CBDB1 [PDF]

Applied and Environmental Microbiology, 2007
ABSTRACT A chlorobenzene reductive dehalogenase of the anaerobic dehalorespiring bacterium Dehalococcoides sp. strain CBDB1 was identified. Due to poor biomass yields, standard protein isolation procedures were not applicable. Therefore, cell extracts from cultures grown on trichlorobenzenes were separated
Adrian, L., Rahnenführer, J., Gobom, J., Hölscher, T.   +3 more
openaire   +3 more sources

Addition of Shewanella oneidensis MR-1 to the Dehalococcoides-containing culture enhances the trichloroethene dechlorination

Environment International, 2019
Dehalococcoides is able to completely dehalogenate tetrachloroethene (PCE) and trichloroethene (TCE) to ethene (ETH). However, the dechlorination efficiency of Dehalococcoides is low and result in the accumulation of toxic intermediates.
Yaru Li, Li-Lian Wen, He-Ping Zhao, Lizhong Zhu   +3 more
doaj   +1 more source

Quantification of genes and gene transcripts for microbial perchlorate reduction in fixed‐bed bioreactors [PDF]

, 2012
Aims:  Optimization of full‐scale, biological perchlorate treatment processes for drinking water would benefit from knowledge of the location and quantity of perchlorate‐reducing bacteria (PRB) and expression of perchlorate‐related genes in bioreactors.
Bender, Bender, Brown, Brown, Brown, Chaudhuri, Choi, De Long, Egert, Giblin, Greer, Hautman, Johnson, Kim, Kirisits, Laurent, Laurent, Lee, Li, Logan, London, Maixner, Marsh, Min, Nerenberg, Nozawa-Inoue, Nozawa-Inoue, Renner, Song, Staley, Webster, Wolff, Xu, Zhang   +33 more
core   +1 more source

Development of a Continuous, Uncoupled Fluorescence‐based Assay for Monitoring Methyltransferase Activity: Methylation Going Sour

ChemCatChem, Volume 18, Issue 3, 12 February 2026.
Methylation going sour: The first uncoupled fluorescence‐based assay was established to measure methyltransferase activity in a continuous fashion. Different use cases are presented, including the assay's compatibility with catalyst formulations other than purified enzymes, the determination of kinetic parameters, as well as the screening of a ...
Benjamin Panagiotis Chapple, Lisa M. Böhmer, Jörg Pietruszka   +2 more
wiley   +1 more source