Results 91 to 100 of about 1,760,212 (203)

Selection of reference genes in canine uterine tissues

Genetics and Molecular Research, 2016
Real-time quantitative polymerase chain reaction (RT-qPCR) is usually employed in gene expression studies in veterinary research, including in studies on canine pyometra. Canine pyometra is a common clinical disease in bitches. When using RT-qPCR, internal standards, such as reference genes, are necessary to investigate relative gene expression by ...
M, Du, X, Wang, Y W, Yue, P Y, Zhou, W, Yao, X, Li, X B, Ding, X F, Liu, H, Guo, W Z, Ma   +9 more
openaire   +2 more sources

Selection of internal reference genes for SYBR green qRT-PCR studies of rhesus monkey () tissues [PDF]

, 2008
Background: The rhesus monkey (Macaca mulatta) is a valuable and widely used model animal for biomedical research. However, quantitative analyses of rhesus gene expression profiles under diverse experimental conditions are limited by a shortage of ...
K Ahn, Jae Won Huh, Sang Je Park, Dae Soo Kim, H S Ha, Y J Kim, J R Lee, Kyu Tae Chang, H S Kim   +8 more
core   +1 more source

Evaluation of candidate reference genes for gene expression research in Vespula vulgaris. [PDF]

Front Insect Sci
IntroductionVespula vulgaris is an invasive wasp that causes considerable detriment to native birds and invertebrates in New Zealand. Reducing at least 80% of invasive wasp densities is necessary to manage the problems this species presents to its invaded range. To explore the function of target genes for the genetic management of V.
McLaughlin GM.
europepmc   +4 more sources

Determination of qPCR Reference Genes Suitable for Normalizing Gene Expression in a Canine Model of Duchenne Muscular Dystrophy [PDF]

Background:Dogs with dystrophin-deficient muscular dystrophy are valuable models of the equivalent human disease, Duchenne Muscular Dystrophy (DMD): unlike the mdx mouse, these animals present a disease severity and progression that closely matches that ...
Aartsma-Rus, Ali, Andersen, Bages, Bello, Bish, Breitbart, Brinkmeyer-Langford, Burton, Bustin, Bustin, Camerino, Cohn, Cotten, Craig, Crist, Duan, Falzarano, Feder, Fjeldbo, Flanigan, Galindo, Gong, Hildyard, Kallestad, Kornegay, Kornegay, Li, Liu, Mah, McGreevy, Nghiem, Pegoraro, Pfaffl, Piva, Reinig, Rho, Robinson-Hamm, Robriquet, Schmittgen, Solano, Spassov, Swaggart, Thellin, Vieira, Vieira Natassia, Walmsley, Webster, Wuebbles, Yu, Zammit   +50 more
core   +1 more source

Selection and Validation of Reference Genes in Dendrocalamus brandisii for Quantitative Real-Time PCR

Plants
Dendrocalamus brandisii (Munro) Kurz is a sympodial bamboo species with a wide distribution in tropical and subtropical regions. Due to its remarkable regenerative ability and exceptional flavor, this species plays a pivotal role in bolstering the ...
Jutang Jiang, Changhong Mu, Yucong Bai, Wenlong Cheng, Ruiman Geng, Junlei Xu, Yuping Dou, Zhanchao Cheng, Jian Gao   +8 more
doaj   +1 more source

Evaluation of reference genes for gene expression studies in pig muscle tissue by real-time PCR

Czech Journal of Animal Science, 2011
The selection of reference genes is essential for gene expression studies when using a real-time quantitative polymerase chain reaction (PCR). Reference gene selection should be performed for each experiment because the gene expression level may be ...
M. Nesvadbová, A. Knoll
doaj   +1 more source

Validation of Reference Genes for Robust qRT-PCR Gene Expression Analysis in the Rice Blast Fungus Magnaporthe oryzae [PDF]

, 2017
This is the final version of the article. Available from Public Library of Science via the DOI in this record.The rice blast fungus causes significant annual harvest losses. It also serves as a genetically-tractable model to study fungal ingress.
Bentley, MA, Che Omar, S, Gurr, SJ, Morieri, G, Preston, GM   +4 more
core   +1 more source

Selection of Reference Genes in Siraitia siamensis and Expression Patterns of Genes Involved in Mogrosides Biosynthesis

Plants
Siraitia siamensis is a traditional Chinese medicinal herb. In this study, using S. siamensis cultivated in vitro, twelve candidate reference genes under various treatments were analyzed for their expression stability by using algorithms such as GeNorm ...
Wenqiang Chen, Xiaodong Lin, Yan Wang, Detian Mu, Changming Mo, Huaxue Huang, Huan Zhao, Zuliang Luo, Dai Liu, Iain W. Wilson, Deyou Qiu, Qi Tang   +11 more
doaj   +1 more source

Reference Standards for Gene and Cell Therapy Products [PDF]

Molecular Therapy, 2017
Reference standards are important tools for the calibration of medical products and procedures. They are particularly important when dosing and potency are critical parameters for patient safety and product efficacy.1 Reference standards benefit the field by fostering best practices for the manufacturing and testing of safe and efficacious medicinal ...
openaire   +2 more sources

Validation and Evaluation of Reference Genes for Quantitative Real-Time PCR Analysis in Mythimna loreyi (Lepidoptera: Noctuidae)

Insects
Quantitative real-time PCR (qRT-PCR) is a widely applied technique for accurately assessing the expression of target genes. In practice, the evaluation of gene expression requires appropriate reference genes.
Liuyang Wang, Chaoxia Yang, Qingyu Liu, Xiaofang Zhang, Xiangdong Mei, Tao Zhang, Jun Ning   +6 more
doaj   +1 more source