Results 121 to 130 of about 1,765,900 (302)

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

Validation and Evaluation of Reference Genes for Quantitative Real-Time PCR Analysis in Mythimna loreyi (Lepidoptera: Noctuidae)

Insects
Quantitative real-time PCR (qRT-PCR) is a widely applied technique for accurately assessing the expression of target genes. In practice, the evaluation of gene expression requires appropriate reference genes.
Liuyang Wang, Chaoxia Yang, Qingyu Liu, Xiaofang Zhang, Xiangdong Mei, Tao Zhang, Jun Ning   +6 more
doaj   +1 more source

The role of histone modifications in transcription regulation upon DNA damage

FEBS Letters, EarlyView.
This review discusses the critical role of histone modifications in regulating gene expression during the DNA damage response (DDR). By modulating chromatin structure and recruiting repair factors, these post‐translational modifications fine‐tune transcriptional programmes to maintain genomic stability.
Angelina Job Kolady, Siyao Wang
wiley   +1 more source

Substrate specificity of Burkholderia pseudomallei multidrug transporters is influenced by the hydrophilic patch in the substrate‐binding pocket

FEBS Letters, EarlyView.
Multidrug transporters BpeB and BpeF from the Gram‐negative pathogen Burkholderia pseudomallei have a hydrophilic patch in their substrate‐binding pocket. Drug susceptibility tests and growth curve analyses using an Escherichia coli recombinant expression system revealed that the hydrophilic patches of BpeB and BpeF are involved in the substrate ...
Ui Okada, Satoshi Murakami
wiley   +1 more source

Selection of Reference Genes for the Normalization of RT-qPCR Data in Gene Expression Studies in Insects: A Systematic Review

Frontiers in Physiology, 2018
Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is a reliable technique for quantifying expression levels of targeted genes during various biological processes in numerous areas of clinical and biological research.
Jing Lü, Chunxiao Yang, Youjun Zhang, Huipeng Pan   +3 more
doaj   +1 more source

Determination of qPCR Reference Genes Suitable for Normalizing Gene Expression in a Canine Model of Duchenne Muscular Dystrophy [PDF]

Background:Dogs with dystrophin-deficient muscular dystrophy are valuable models of the equivalent human disease, Duchenne Muscular Dystrophy (DMD): unlike the mdx mouse, these animals present a disease severity and progression that closely matches that ...
Aartsma-Rus, Ali, Andersen, Bages, Bello, Bish, Breitbart, Brinkmeyer-Langford, Burton, Bustin, Bustin, Camerino, Cohn, Cotten, Craig, Crist, Duan, Falzarano, Feder, Fjeldbo, Flanigan, Galindo, Gong, Hildyard, Kallestad, Kornegay, Kornegay, Li, Liu, Mah, McGreevy, Nghiem, Pegoraro, Pfaffl, Piva, Reinig, Rho, Robinson-Hamm, Robriquet, Schmittgen, Solano, Spassov, Swaggart, Thellin, Vieira, Vieira Natassia, Walmsley, Webster, Wuebbles, Yu, Zammit   +50 more
core   +1 more source

Identification of Four Soybean Reference Genes for Gene Expression Normalization

The Plant Genome, 2008
Gene expression analysis requires the use of reference genes constitutively expressed independently of tissues or environmental conditions. Housekeeping genes (e.g., actin, tubulin, ribosomal, polyubiquitin, and elongation factor 1‐α) are commonly used as reference genes with the assumption that they are uniformly expressed.
M. Libault, S. Thibivilliers, D. D. Bilgin, O. Radwan, M. Benitez, S. J. Clough, G. Stacey   +6 more
openaire   +2 more sources

Mechanisms of parasite‐mediated disruption of brain vessels

FEBS Letters, EarlyView.
Parasites can affect the blood vessels of the brain, often causing serious neurological problems. This review explains how different parasites interact with and disrupt these vessels, what this means for brain health, and why these processes matter. Understanding these mechanisms may help us develop better ways to prevent or treat brain infections in ...
Leonor Loira, Sílvia Arroz‐Madeira, Cláudio A. Franco, Sara Silva Pereira   +3 more
wiley   +1 more source

Evaluation of eleven reference genes for Reverse Transcriptase Quantitative PCR of rubber tree under water deficit [PDF]

, 2015
Reverse Transcriptase Quantitative PCR (RTqPCR) is a powerful technique in order to detect low abundance of mRNA in the plant cell. The measurement of transcript abundance is relative to the control of expression such as housekeeping genes.
Leclercq, Julie, Montoro, Pascal, Putranto, Riza Arief   +2 more
core  

Time after time – circadian clocks through the lens of oscillator theory

FEBS Letters, EarlyView.
Oscillator theory bridges physics and circadian biology. Damped oscillators require external drivers, while limit cycles emerge from delayed feedback and nonlinearities. Coupling enables tissue‐level coherence, and entrainment aligns internal clocks with environmental cues.
Marta del Olmo, Carolin Ector, Hanspeter Herzel   +2 more
wiley   +1 more source