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Evaluation of reference genes for qPCR in human liver and kidney tissue from individuals with obesity. [PDF]

open access: yesSci Rep
Romaniuk-Drapała A   +5 more
europepmc   +1 more source

Selection and Validation of Stable Reference Genes for RT-qPCR Analyses of <i>Rumex patientia</i> (Polygonaceae) Under Four Abiotic Stresses. [PDF]

open access: yesGenes (Basel)
Yang Q   +11 more
europepmc   +1 more source
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Candidate reference genes for quantitative gene expression analysis in Lagerstroemia indica

Molecular Biology Reports, 2021
Quantitative gene expression analysis by qPCR requires reference genes for normalization. Lagerstroemia indica (crape myrtle) is a popular ornamental plant in the world, but suitable endogenous reference genes are lacking. To find suitable reference genes, we evaluated the stabilities of nine candidate genes in six experimental data sets: six different
Manli Chen   +6 more
openaire   +2 more sources

Candidate reference genes for gene expression studies in water lily

Analytical Biochemistry, 2010
The selection of an appropriate reference gene(s) is a prerequisite for the proper interpretation of quantitative Real-Time polymerase chain reaction data. We report the evaluation of eight candidate reference genes across various tissues and treatments in the water lily by the two software packages geNorm and NormFinder.
Huolin, Luo   +5 more
openaire   +2 more sources

Identification of Stable Reference Genes for Toxicogenomic and Gene Expression Analysis

2021
Gene expression analysis has been becoming a popular method for studying gene function and response to different environmental stresses, including toxin/pollution exposure. Selection of a suitable reference gene is critically important for gene expression analysis due to that wrong reference genes will cause misleading and even wrong conclusion. A good
Xiaoping, Pan, Baohong, Zhang
openaire   +2 more sources

Selection of reference genes for gene expression studies in astrocytomas

Analytical Biochemistry, 2011
This study was aimed to test a panel of six housekeeping genes (GAPDH, HPRT1, POLR2A, RPLP0, ACTB, and H3F) so as to identify and validate the most suitable reference genes for expression studies in astrocytomas. GAPDH was the most stable and HPRT1 was the least stable reference gene.
Sylwia M, Gresner   +5 more
openaire   +2 more sources

Rice Reference Genes: redefining reference genes in rice by mining RNA-seq datasets

Plant and Cell Physiology
Abstract Reverse transcription quantitative real-time PCR (RT-qPCR) is esteemed for its precision and reliability, positioning it as the standard for evaluating gene expression. Selecting suitable reference genes is crucial for acquiring accurate data on target gene expression.
Xin Liu   +9 more
openaire   +2 more sources

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