Results 101 to 110 of about 2,631 (175)

Reference gene stability of a synanthropic fly, Chrysomya megacephala

Parasites & Vectors, 2015
Background Stable reference genes are essential for accurate normalization in gene expression studies with reverse transcription quantitative polymerase chain reaction (qPCR).
Xiaoyun Wang, Mei Xiong, Jialu Wang, Chaoliang Lei, Fen Zhu   +4 more
doaj   +1 more source

RefFinder based on the rankings from Delta CT, BestKeeper, Normfinder, Genorm each program, It assigns an appropriate weight to an individual gene and calculated the geometric mean of their weights for the overall final ranking.

, 2012
RefFinder based on the rankings from Delta CT, BestKeeper, Normfinder, Genorm each program, It assigns an appropriate weight to an individual gene and calculated the geometric mean of their weights for the overall final ranking.
Ladislav Kučera (145943), Ilona Svobodová (145954), Jaroslava Ovesná (145940), Luigi Milella (145957), Kateřina Vaculová (145946), Kamila Štrymplová (145950)   +5 more
core   +1 more source

Enhancing gene expression studies in bovine embryos fertilized in vitro: Identifying stable reference genes across blastocysts with different developmental speeds

The Journal of Reproduction and Development
In vitro fertilization (IVF) is crucial for livestock reproduction; however, pregnancy rates after embryo transfer vary depending on the developmental speeds of the embryos. Although quantitative PCR (qPCR) is used to predict developmental potential, its
Sung-Ho KIM, Sang-Yup LEE, Saet-Byul KIM, Tae-Gyun KIM, Min JANG, Sung-Ho YUN, Seong-Eun HEO, Do-Yoon KIM, Seung-Joon KIM, Won-Jae LEE   +9 more
doaj   +1 more source

Rank of reference genes based on their expression stability in different developmental stages, castes and tissues of S. invicta according to geNorm, BestKeeper, NormFinder and RefFinder analysis.

, 2013
Rank of reference genes based on their expression stability in different developmental stages, castes and tissues of S. invicta according to geNorm, BestKeeper, NormFinder and RefFinder analysis.
Daifeng Cheng (384012), Xiaofang He (384013), Zhiling Zhang (301340), Guangwen Liang (297718)   +3 more
core   +1 more source

Expression stability rankings order of all sixteen- finger millet candidate references genes validated from five different tools: Delta Ct (ΔCT), geNorm (GN), NormFinder (NF), BestKeeper (BK), and RefFinder (RF).

, 2018
Expression stability rankings order of all sixteen- finger millet candidate references genes validated from five different tools: Delta Ct (ΔCT), geNorm (GN), NormFinder (NF), BestKeeper (BK), and RefFinder (RF).
Kummari Divya (5856101), Mahamaya G. Dhaware (5856095), Dumbala Srinivas Reddy (475340), Bommineni Pradeep Reddy (5856098), Pooja Bhatnagar-Mathur (475341), Palakolanu Sudhakar Reddy (530583), Kiran K. Sharma (475343)   +6 more
core   +1 more source

Summary of the best combination of reference genes for accurate normalization across five experimental sets of pearl millet using geNorm and RefFinder programs.

, 2014
a Pairwise variation (V) represents the optimal combination of reference control genes required to pass the suggested cut-off value 0.15 [31]. A single common reference control gene for expression study across experimental sets is highlighted in gray ...
Eduardo Blumwald (25796), Prasenjit Saha (623445)   +1 more
core   +1 more source

Seasonal Stability Assessment of Reference Genes for Quantitative Real-Time Polymerase Chain Reaction Normalization in Bombus terrestris

Current Issues in Molecular Biology
Bumblebees (B. terrestris) play a crucial role as highly efficient biological agents in commercial pollination. Understanding the molecular mechanisms governing their adaptation to diverse seasonal environments may pave the way for effective management ...
Kathannan Sankar, Kyeong-Yong Lee, Kyu-Won Kwak, Su-Jin Lee, Young-Bo Lee   +4 more
doaj   +1 more source

Screening of Reference Genes for Quantitative Real-time PCR in Curcuma alismatifolia Bracts

Guangdong nongye kexue
【Objective】To screen suitable reference genes for Quantitative Real-time PCR(qRT-PCR) analysis in Curcuma alismatifolia bract, establishing the theoretical foundation for investigating the functional genes associated with bract color.【Method】Nine C ...
Jianjun TAN, Lishan HUANG, Yiwei ZHOU, Yechun XU, Yuanjun YE   +4 more
doaj   +1 more source

Stability rankings of candidate reference genes calculated by geNorm, NormFinder, and BestKeeper algorithms.

, 2018
Comprehensive ranking of candidate reference genes using RefFinder.
Jason G. Walling (5184296), Marcus A. Vinje (5184302), Leslie A. Zalapa (5184299)   +2 more
core   +1 more source

Identification of specific reference gene for normalization of RT-qPCR data in rhythmic gene expression studies of the effect of developmental hormone antagonist in postembryonic development in Bombyx mori

Frontiers in Insect Science
Bombyx mori is a lepidopteran holometabolous insect with distinct developmental stages: egg, larvae, pupae, and adult. The lepidopteran insect undergoes major modifications in the central nervous system (CNS) so as to adapt to the lifestyle of these ...
Minurani Dalai, Anita Jagota
doaj   +1 more source