Results 31 to 40 of about 1,158,325 (299)

MCM2-7 ring closure involves the Mcm5 C-terminus and triggers Mcm4 ATP hydrolysis

Nature Communications
The eukaryotic helicase MCM2-7, is loaded by ORC, Cdc6 and Cdt1 as a double-hexamer onto replication origins. The insertion of DNA into the helicase leads to partial MCM2-7 ring closure, while ATP hydrolysis is essential for consecutive steps in pre ...
Sarah V. Faull, Marta Barbon, Audrey Mossler, Zuanning Yuan, Lin Bai, L. Maximilian Reuter, Alberto Riera, Christian Winkler, Indiana Magdalou, Matthew Peach, Huilin Li, Christian Speck   +11 more
doaj   +1 more source

Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid‐derived monolayers compared to HeLa cells

FEBS Letters, EarlyView.
Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi, Pilar Samperio Ventayol, Natalie Burkard, Nicolas Schlegel, Carmen Aguilar, Sina Bartfeld   +5 more
wiley   +1 more source

Plasmodium falciparum gametogenesis essential protein 1 (GEP1) is a transmission‐blocking target

FEBS Letters, EarlyView.
This study shows Plasmodium falciparum GEP1 is vital for activating sexual stages of malarial parasites even independently of a mosquito factor. Knockout parasites completely fail gamete formation even when a phosphodiesterase inhibitor is added. Two single‐nucleotide polymorphisms (V241L and S263P) are found in 12%–20% of field samples.
Frederik Huppertz, Milagros Siebeck Caturelli, Lina S. Lehmann, Florian Kurth, Alexander G. Maier, Kai Matuschewski   +5 more
wiley   +1 more source

Kinetic Studies on the Interaction of HIV-1 Gag Protein with the HIV-1 RNA Packaging Signal

Viruses
During HIV-1 virus assembly, the genomic RNA (vRNA) is selected for packaging by the viral protein Gag because it contains a specific packaging signal, Psi. While there have been numerous studies of Gag–Psi interactions, there is almost no information on
Constance Rink, Tomas Kroupa, Siddhartha A. K. Datta, Alan Rein   +3 more
doaj   +1 more source

Cdt1 stabilizes an open MCM ring for helicase loading

Nature Communications, 2017
The loading and activation of the Mcm2-7 replicative helicase couples cell cycle progression to DNA replication. Here the authors use X-ray crystallography and single-particle electron microscopy to demonstrate how Ctd1 functions to promote MCM loading ...
Jordi Frigola, Jun He, Kerstin Kinkelin, Valerie E. Pye, Ludovic Renault, Max E. Douglas, Dirk Remus, Peter Cherepanov, Alessandro Costa, John F. X. Diffley   +9 more
doaj   +1 more source

Biophysical analysis of angiotensin II and amyloid‐β cross‐interaction in aggregation and membrane disruption

FEBS Letters, EarlyView.
Angiotensin II (AngII), a neuropeptide, interacts with amyloid‐β (Aβ), a key player in Alzheimer's disease. This study reveals that AngII reduces Aβ aggregation and membrane disruption in vitro. Biophysical assays and molecular modeling suggest AngII binds disordered Aβ forms, potentially modulating early amyloidogenic events and contributing to ...
Mohsen Habibnia, Eric Catalina‐Hernandez, Maialen Cabrerizo‐Idiazabal, Ramon Barnadas‐Rodríguez, Mario Lopez‐Martin, Alex Peralvarez‐Marin   +5 more
wiley   +1 more source

Reconstitution of human DNA licensing and the structural and functional analysis of key intermediates

Nature Communications
Human DNA licensing initiates replication fork assembly and DNA replication. This reaction promotes the loading of the hMCM2-7 complex on DNA, which represents the core of the replicative helicase that unwinds DNA during S-phase.
Jennifer N. Wells, Lucy V. Edwardes, Vera Leber, Shenaz Allyjaun, Matthew Peach, Joshua Tomkins, Antonia Kefala-Stavridi, Sarah V. Faull, Ricardo Aramayo, Carolina M. Pestana, Lepakshi Ranjha, Christian Speck   +11 more
doaj   +1 more source

Elements in the 5′ Untranslated Region of Viral RNA Important for HIV Gag Recognition and Cross-Packaging

Viruses
During retrovirus assembly, Gag packages unspliced viral RNA as the virion genome. Genome packaging is usually specific with occasional exceptions of cross-packaging RNA from distantly related retroviruses.
Zetao Cheng, Olga A. Nikolaitchik, Alice Duchon, Jonathan M. O. Rawson, Vinay K. Pathak, Wei-Shau Hu   +5 more
doaj   +1 more source

The Replication Database: Documenting the Replicability of Psychological Science [PDF]

Journal of Open Psychology Data
In psychological science, replicability—repeating a study with a new sampleachieving consistent results (Parsons et al., 2022)—is critical for affirming the validity of scientific findings. Despite its importance, replication efforts are few and far between in psychological science with many attempts failing to corroborate past findings. This scarcity,
Lukas Röseler, Leonard Kaiser, Christopher Doetsch, Noah Klett, Christian Seida, Astrid Schütz, Balazs Aczel, Nadia Adelina, Valeria Agostini, Samuel Alarie, Nihan Albayrak-Aydemir, Alaa Aldoh, Ali H. Al-Hoorie, Flavio Azevedo, Bradley J. Baker, Charlotte Lilian Barth, Julia Beitner, Cameron Brick, Hilmar Brohmer, Subramanya Prasad Chandrashekar, Kai Li Chung, Jamie P. Cockcroft, Jamie Cummins, Veronica Diveica, Tsvetomira Dumbalska, Emir Efendic, Mahmoud Elsherif, Thomas Evans, Gilad Feldman, Adrien Fillon, Nico Förster, Joris Frese, Oliver Genschow, Vaitsa Giannouli, Biljana Gjoneska, Timo Gnambs, Amélie Gourdon-Kanhukamwe, Christopher J. Graham, Helena Hartmann, Clove Haviva, Alina Herderich, Leon P. Hilbert, Darías Holgado, Ian Hussey, Zlatomira G. Ilchovska, Tamara Kalandadze, Veli-Matti Karhulahti, Leon Kasseckert, Maren Klingelhöfer-Jens, Alina Koppold, Max Korbmacher, Louisa Kulke, Niclas Kuper, Annalise LaPlume, Gavin Leech, Feline Lohkamp, Nigel Mantou Lou, Dermot Lynott, Maximilian Maier, Maria Meier, Maria Montefinese, David Moreau, Kellen Mrkva, Monika Nemcova, Danna Oomen, Julian Packheiser, Shubham Pandey, Frank Papenmeier, Mariola Paruzel-Czachura, Yuri G. Pavlov, Zoran Pavlović, Charlotte R. Pennington, Merle-Marie Pittelkow, Willemijn Plomp, Paul E. Plonski, Ekaterina Pronizius, Andrew Adrian Pua, Katarzyna Pypno-Blajda, Manuel Rausch, Tobias R. Rebholz, Elena Richert, Jan Philipp Röer, Robert Ross, Kathleen Schmidt, Aleksandrina Skvortsova, Matthias F. J. Sperl, Alvin W. M. Tan, J. Lukas Thürmer, Aleksandra Tołopiło, Wolf Vanpaemel, Leigh Ann Vaughn, Steven Verheyen, Lukas Wallrich, Lucia Weber, Julia K. Wolska, Mirela Zaneva, Yikang Zhang   +96 more
openaire   +13 more sources

4‐nitrobenzoate inhibits 4‐hydroxybenzoate polyprenyltransferase in malaria parasites and enhances atovaquone efficacy

FEBS Letters, EarlyView.
Atovaquone is an antimalarial requiring potentiation for sufficient efficacy. We pursued strategies to enhance its activity, showing that 4‐nitrobenzoate inhibits 4‐hydroxybenzoate polyprenyltransferase, decreasing ubiquinone biosynthesis. Since atovaquone competes with ubiquinol in mitochondria, 4‐nitrobenzoate facilitates its action, potentiating ...
Ignasi Bofill Verdaguer, Matheus Felipe Santos, Maurício Mazzine Filho, Gabriela Oliveira Castro, Agustín Hernández, Manoel Aparecido Peres, Alejandro Miguel Katzin, Marcell Crispim   +7 more
wiley   +1 more source