The DNA helicase Pfh1 promotes fork merging at replication termination sites to ensure genome stability [PDF]
, 2012 Bidirectionally moving DNA replication forks merge at termination sites composed of accidental or programmed DNA-protein barriers. If merging fails, then regions of unreplicated DNA can result in the breakage of DNA during mitosis, which in turn can give
Steinacher, R. +14 more
core +1 more source
Murine leukemia virus (MLV) replication monitored with fluorescent proteins [PDF]
, 2006 Background: Cancer gene therapy will benefit from vectors that are able to replicate in tumor tissue and cause a bystander effect. Replication-competent murine leukemia virus (MLV) has been described to have potential as cancer therapeutics, however, MLV
Erlwein, Otto +3 more
core +1 more source
Influence of retinoblastoma-related gene silencing on the initiation of DNA replication by African cassava mosaic virus Rep in cells of mature leaves in Nicotiana benthamiana plants [PDF]
, 2011 Background Geminiviruses mainly infect terminally differentiated tissues and cells in plants. They need to reprogramme host cellular machinery for DNA replication.
Yule Liu +14 more
core +1 more source
Is RecF a DNA replication protein? [PDF]
Proceedings of the National Academy of Sciences, 1997 Maintenance of genetic integrity is of foremost importance to the cell. Thus, cells are well equipped with a number of repair mechanisms for different types of DNA damage. All DNA polymerases are very sensitive to pyrimidine dimers, a major DNA lesion of UV irradiation.
openaire +2 more sources
The Viral Replication Complex Is Associated with the Virulence of Newcastle Disease Virus [PDF]
, 2010 Virulent strains of Newcastle disease virus ([NDV] also known as avian paramyxovirus type 1) can be discriminated from low-virulence strains by the presence of multiple basic amino acid residues at the proteolytic cleavage site of the fusion (F) protein.
Peeters, B.P.H. +7 more
core +1 more source
Acta Pharmaceutica Sinica B, 2019 Bicyclol is a synthetic drug for hepatoprotection in clinic since 2004. Preliminary clinical observations suggest that bicyclol might be active against hepatitis C virus (HCV) with unknown mechanism.
Meng-Hao Huang +15 more
doaj +1 more source
Replication Protein A (RPA) Phosphorylation Prevents RPA Association with Replication Centers [PDF]
Molecular and Cellular Biology, 2004 Mammalian replication protein A (RPA) undergoes DNA damage-dependent phosphorylation at numerous sites on the N terminus of the RPA2 subunit. To understand the functional significance of RPA phosphorylation, we expressed RPA2 variants in which the phosphorylation sites were converted to aspartate (RPA2(D)) or alanine (RPA2(A)).
Vitaly M, Vassin +2 more
openaire +2 more sources
Emerging Microbes and Infections, 2017 The M gene segment of influenza A virus has been shown to be a contributing factor to the high growth phenotype. However, it remains largely unknown why matrix protein 1 (M1), the major structural protein encoded by M gene, exhibits pH-dependent ...
Meng-Jung Chiang +11 more
doaj +1 more source
Dynamic distribution of SeqA protein across the chromosome of Escherichia coli K-12 [PDF]
, 2010 The bacterial SeqA protein binds to hemi-methylated GATC sequences that arise in newly synthesized DNA upon passage of the replication machinery. In Escherichia coli K-12, the single replication origin oriC is a well-characterized target for SeqA, which ...
Stephen J. W. Busby +17 more
core +1 more source
Overproduction of Escherichia coli replication proteins by the use of runaway-replication plasmids [PDF]
Journal of Bacteriology, 1983 A derivative of the runaway-replication plasmid was constructed. This plasmid, pSY343, has the gene for kanamycin resistance and single sites for EcoRI, BamHI, HindIII, KpnI, and XhoI that can be used as cloning sites without inactivating the kanamycin resistance gene or the replication genes.
S, Yasuda, T, Takagi
openaire +2 more sources