USP37 prevents unscheduled replisome unloading through MCM complex deubiquitination [PDF]
Nature CommunicationsThe CMG helicase (CDC45-MCM2-7-GINS) unwinds DNA as a component of eukaryotic replisomes. Replisome (dis)assembly is tightly coordinated with cell cycle progression to ensure genome stability.
Derek L. Bolhuis +7 more
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In Vitro Reconstitution Defines the Minimal Requirements for Cdc48-Dependent Disassembly of the CMG Helicase in Budding Yeast [PDF]
Cell Reports, 2019 Summary: Disassembly of the replisome is the final step of chromosome duplication in eukaryotes. In budding yeast and metazoa, cullin ubiquitin ligases are required to ubiquitylate the Cdc45-MCM-GINS (CMG) helicase that lies at the heart of the replisome,
Progya P. Mukherjee, Karim P.M. Labib
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A Replisome’s journey through the bacterial chromosome [PDF]
Frontiers in Microbiology, 2015 Genome duplication requires the coordinated activity of a multi-component machine, the replisome. In contrast to the background of metabolic diversity across the bacterial domain, the composition and architecture of the bacterial replisome seems to have ...
Thomas Robert Beattie +1 more
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Parental Histone Recycling During Chromatin Replication [PDF]
BiomoleculesThe past decade has seen significant advancement in our understanding of DNA replication-coupled chromatin assembly, especially parental histone recycling that is essential for epigenetic inheritance.
Xin Bi
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The bacteriophage T4 replisome: a model system for understanding DNA replication mechanisms. [PDF]
EcoSal PlusThe bacteriophage T4 replisome is a complex molecular machine responsible for DNA replication in the T4 phage. It consists of multiple proteins that work together to ensure efficient and accurate replication of the phage genome.
Spiering MM, Benkovic SJ.
europepmc +2 more sources
eLife, 2021 R-loops are a major source of genome instability associated with transcription-induced replication stress. However, how R-loops inherently impact replication fork progression is not understood.
Charanya Kumar +3 more
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Replisomes restrict SMC translocation in vivo [PDF]
Nature CommunicationsStructural maintenance of chromosomes (SMC) complexes organize genomes by extruding DNA loops, while replisomes duplicate entire chromosomes. These essential molecular machines must collide frequently in every cell cycle, yet how such collisions are ...
Qin Liao +3 more
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Mechanisms for Maintaining Eukaryotic Replisome Progression in the Presence of DNA Damage
Frontiers in Molecular Biosciences, 2021 The eukaryotic replisome coordinates template unwinding and nascent-strand synthesis to drive DNA replication fork progression and complete efficient genome duplication.
Thomas A. Guilliam
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The replisome guides nucleosome assembly during DNA replication
Cell & Bioscience, 2020 Nucleosome assembly during DNA replication is tightly coupled to ongoing DNA synthesis. This process, termed DNA replication-coupled (RC) nucleosome assembly, is essential for chromatin replication and has a great impact on both genome stability ...
Wenshuo Zhang, Jianxun Feng, Qing Li
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A common mechanism for recruiting the Rrm3 and RTEL1 accessory helicases to the eukaryotic replisome
The EMBO JournalThe eukaryotic replisome is assembled around the CMG (CDC45-MCM-GINS) replicative helicase, which encircles the leading-strand DNA template at replication forks.
Ottavia Olson +3 more
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