Characterizing replisome disassembly in human cells
iScienceSummary: To ensure timely duplication of the entire eukaryotic genome, thousands of replication machineries (replisomes) act on genomic DNA at any time during S phase. In the final stages of this process, replisomes are unloaded from chromatin. Unloading
Rebecca M. Jones +10 more
doaj +7 more sources
Speed variations of bacterial replisomes
eLife, 2022 Replisomes are multi-protein complexes that replicate genomes with remarkable speed and accuracy. Despite their importance, their dynamics is poorly characterized, especially in vivo.
Deepak Bhat +4 more
doaj +3 more sources
Single-molecule mapping of replisome progression [PDF]
Molecular Cell, 2022 Fundamental aspects of DNA replication, such as the anatomy of replication stall sites, how replisomes are influenced by gene transcription and whether the progression of sister replisomes is coordinated are poorly understood. Available techniques do not
Clémence Claussin +2 more
semanticscholar +3 more sources
Competition for the nascent leading strand shapes the requirements for PCNA loading in the replisome. [PDF]
EMBO JDuring DNA replication, the DNA polymerases Pol δ and Pol ε utilise the ring-shaped sliding clamp PCNA to enhance their processivity. PCNA loading onto DNA is accomplished by the clamp loaders RFC and Ctf18-RFC, which function primarily on the lagging ...
Fletcher EE, Jones ML, Yeeles JTP.
europepmc +2 more sources
The human CTF4-orthologue AND-1 interacts with DNA polymerase α/primase via its unique C-terminal HMG box [PDF]
Open Biology, 2017 A dynamic multi-protein assembly known as the replisome is responsible for DNA synthesis in eukaryotic cells. In yeast, the hub protein Ctf4 bridges DNA helicase and DNA polymerase and recruits factors with roles in metabolic processes coupled to DNA ...
Mairi L. Kilkenny +5 more
doaj +3 more sources
LRR1-mediated replisome disassembly promotes DNA replication by recycling replisome components
Journal of Cell Biology, 2021 Fan et al. show that replisome disassembly during S phase is required for efficient DNA replication by recycling essential replisome components between chromatin-bound and soluble compartments.
Yilin Fan +6 more
semanticscholar +3 more sources
TRAIP drives replisome disassembly and mitotic DNA repair synthesis at sites of incomplete DNA replication [PDF]
eLife, 2019 The faithful segregation of eukaryotic chromosomes in mitosis requires that the genome be duplicated completely prior to anaphase. However, cells with large genomes sometimes fail to complete replication during interphase and instead enter mitosis with ...
Bhowmick, Rahul +5 more
core +4 more sources
Single-Strand DNA Breaks Cause Replisome Disassembly [PDF]
Molecular Cell, 2020 DNA damage impedes replication fork progression and threatens genome stability. Upon encounter with most DNA adducts, the replicative CMG helicase (CDC45-MCM2-7-GINS) stalls or uncouples from the point of synthesis, yet CMG eventually resumes replication.
K. Vrtis +9 more
semanticscholar +3 more sources
Torsion is a dynamic regulator of DNA replication stalling and reactivation [PDF]
Nature CommunicationsDNA’s helical structure necessitates replisome rotation relative to DNA during replication, creating inevitable topological challenges. How replication generates and overcomes torsional stress remains unclear.
Xiaomeng Jia +9 more
doaj +2 more sources
The Interplay of Cohesin and the Replisome at Processive and Stressed DNA Replication Forks
Cells, 2021 The cohesin complex facilitates faithful chromosome segregation by pairing the sister chromatids after DNA replication until mitosis. In addition, cohesin contributes to proficient and error-free DNA replication.
Janne J. M. van Schie, Job de Lange
doaj +2 more sources