Results 21 to 30 of about 34,336 (231)

Replisome passage through the cohesin ring

bioRxiv
Following eukaryotic genome replication, the two newly synthesised sister chromatids remain paired by the ring-shaped cohesin complex, enabling their faithful segregation to daughter cells during cell divisions1.
Glaser S, Molodtsov MI, Diffley JFX, Uhlmann F.   +3 more
europepmc   +2 more sources

USP37 prevents premature disassembly of stressed replisomes by TRAIP [PDF]

Nature Communications
The eukaryotic replisome, which consists of the CDC45-MCM2-7-GINS (CMG) helicase, replicative polymerases, and several accessory factors, sometimes encounters proteinaceous obstacles that threaten genome integrity.
Olga V. Kochenova, Giuseppina D’Alessandro, Domenic Pilger, Ernst Schmid, Sean L. Richards, Marcos Rios Garcia, Satpal S. Jhujh, Andrea Voigt, Vipul Gupta, Christopher J. Carnie, R. Alex Wu, Nadia Gueorguieva, Simon Lam, Grant S. Stewart, Johannes C. Walter, Stephen P. Jackson   +15 more
doaj   +2 more sources

The Fate of Two Unstoppable Trains After Arriving Destination: Replisome Disassembly During DNA Replication Termination

Frontiers in Cell and Developmental Biology, 2021
In eukaryotes, the perfect duplication of the chromosomes is executed by a dynamic molecular machine called the replisome. As a key step to finishing DNA replication, replisome disassembly is triggered by ubiquitylation of the MCM7 subunit of the ...
Yisui Xia
doaj   +1 more source

DNA replication machinery: Insights from in vitro single-molecule approaches

Computational and Structural Biotechnology Journal, 2021
The replisome is the multiprotein molecular machinery that replicates DNA. The replisome components work in precise coordination to unwind the double helix of the DNA and replicate the two strands simultaneously.
Rebeca Bocanegra, G.A. Ismael Plaza, Carlos R. Pulido, Borja Ibarra   +3 more
doaj   +1 more source

Replication of the Mammalian Genome by Replisomes Specific for Euchromatin and Heterochromatin

Frontiers in Cell and Developmental Biology, 2021
Replisomes follow a schedule in which replication of DNA in euchromatin is early in S phase while sequences in heterochromatin replicate late. Impediments to DNA replication, referred to as replication stress, can stall replication forks triggering ...
Jing Zhang, Marina A. Bellani, Jing Huang, Ryan C. James, Durga Pokharel, Julia Gichimu, Himabindu Gali, Grant Stewart, Michael M. Seidman   +8 more
doaj   +1 more source

Checkpoint kinase interaction with DNA polymerase alpha regulates replication progression during stress [version 1; peer review: 3 approved]

Wellcome Open Research, 2023
Background: In eukaryotes, replication stress activates a checkpoint response, which facilitates genome duplication by stabilising the replisome. How the checkpoint kinases regulate the replisome remains poorly understood.
Andreas Hadjicharalambous, Philip Zegerman, Geylani Can, Alex J. Whale, Jonathan M. Houseley, J. Mark Skehel   +5 more
doaj   +1 more source

Separase prevents genomic instability by controlling replication fork speed [PDF]

, 2016
Proper chromosome segregation is crucial for preserving genomic integrity, and errors in this process cause chromosome mis-segregation, which may contribute to cancer development.
Cucco, Francesco, Palumbo, Elisa, Camerini, Serena, D'Alessio, Barbara, Quarantotti, Valentina, Casella, Maria Luisa, Rizzo, Ilaria Maria, Cukrov, Dubravka, Delia, Domenico, Russo, Antonella, Crescenzi, Marco, Musio, Antonio   +11 more
core   +6 more sources

Mechanisms of eukaryotic replisome disassembly [PDF]

Biochemical Society Transactions, 2020
DNA replication is a complex process that needs to be executed accurately before cell division in order to maintain genome integrity. DNA replication is divided into three main stages: initiation, elongation and termination. One of the key events during initiation is the assembly of the replicative helicase at origins of replication, and this mechanism
Sara Priego Moreno, Agnieszka Gambus
openaire   +2 more sources

Structure of a human replisome shows the organisation and interactions of a DNA replication machine

EMBO Journal, 2021
The human replisome is an elaborate arrangement of molecular machines responsible for accurate chromosome replication. At its heart is the CDC45‐MCM‐GINS (CMG) helicase, which, in addition to unwinding the parental DNA duplex, arranges many proteins ...
Morgan L. Jones, Yasemin Baris, Martin R. G. Taylor, Joseph T. P. Yeeles   +3 more
semanticscholar   +1 more source

Replisome dysfunction upon inducible TIMELESS degradation synergizes with ATR inhibition to trigger replication catastrophe

Nucleic Acids Research, 2023
The structure of DNA replication forks is preserved by TIMELESS (TIM) in the fork protection complex (FPC) to support seamless fork progression. While the scaffolding role of the FPC to couple the replisome activity is much appreciated, the detailed ...
Jeetesh V. Patel, Camryn Zezelic, Julie Rageul, Joanne Saldanha, Arafat Khan, Hyungjin Kim   +5 more
semanticscholar   +1 more source