Results 51 to 60 of about 34,336 (231)
Direct visualization of translesion DNA synthesis polymerase IV at the replisome
bioRxiv, 2022 In bacterial cells, DNA damage tolerance is manifested by the action of translesion DNA polymerases that can synthesize DNA across template lesions that typically block the replicative DNA polymerase III.
P. M. Tuan +3 more
semanticscholar +1 more source
A Proteomic Characterization of Factors Enriched at Nascent DNA Molecules
Cell Reports, 2013 DNA replication is facilitated by multiple factors that concentrate in the vicinity of replication forks. Here, we developed an approach that combines the isolation of proteins on nascent DNA chains with mass spectrometry (iPOND-MS), allowing a ...
Andres J. Lopez-Contreras +10 more
doaj +1 more source
Structures and operating principles of the replisome [PDF]
Science, 2019 Structures of the simplest replisome The DNA replisome performs concerted parental-strand separation and DNA synthesis on both strands. Gao et al. report the cryo–electron microscopy structures of the minimum set of bacteriophage T7 proteins that can carry out leading- and ...
Yang Gao +7 more
semanticscholar +5 more sources
Stochastic association of neighboring replicons creates replication factories in budding yeast [PDF]
, 2013 Peer reviewedPublisher ...
Alessandro P.S. de Moura +66 more
core +2 more sources
, 2019 Maintenance of genome integrity is a key process in all organisms. DNA polymerases (Pols) are central players in this process as they are in charge of the faithful reproduction of the genetic information, as well as of DNA repair.
De Veylder, Lieven +2 more
core +3 more sources
The architecture of a eukaryotic replisome [PDF]
Nature Structural & Molecular Biology, 2015 At the eukaryotic DNA replication fork, it is widely believed that the Cdc45-Mcm2-7-GINS (CMG) helicase is positioned in front to unwind DNA and that DNA polymerases trail behind the helicase. Here we used single-particle EM to directly image a Saccharomyces cerevisiae replisome. Contrary to expectations, the leading strand Pol ɛ is positioned ahead of
Jingchuan, Sun +6 more
openaire +2 more sources
Interfacial instability and DNA fork reversal by repair proteins [PDF]
, 2009 A repair protein like RecG moves the stalled replication fork in the direction from the zipped to the unzipped state of DNA. It is proposed here that a softening of the zipped-unzipped interface at the fork results in the front propagating towards the ...
Bhattacharjee, Somendra M.
core +2 more sources
Single-Molecule Studies of the Replisome [PDF]
Annual Review of Biophysics, 2010 Replication of DNA is carried out by the replisome, a multiprotein complex responsible for the unwinding of parental DNA and the synthesis of DNA on each of the two DNA strands. The impressive speed and processivity with which the replisome duplicates DNA are a result of a set of tightly regulated interactions between the replication proteins.
van Oijen, Antoine M, Loparo, Joseph J
openaire +4 more sources
Observing protein dynamics during DNA-lesion bypass by the replisome
Frontiers in Molecular Biosciences, 2022 Faithful DNA replication is essential for all life. A multi-protein complex called the replisome contains all the enzymatic activities required to facilitate DNA replication, including unwinding parental DNA and synthesizing two identical daughter ...
Elise M. Wilkinson +2 more
semanticscholar +1 more source
An orthogonal T7 replisome for continuous hypermutation and accelerated evolution in E. coli.
ScienceSystems that perform continuous hypermutation of designated genes without compromising the integrity of the host genome can substantially accelerate the evolution of new or enhanced protein functions.
Christian S Diercks +7 more
semanticscholar +2 more sources