Plasmid hypermutation using a targeted artificial DNA replisome
Science Advances, 2021 A designed protein complex processively copies one strand of a plasmid with errors to speed up molecular evolution in vivo. Extensive exploration of a protein’s sequence space for improved or new molecular functions requires in vivo evolution with large ...
Xiao Yi +3 more
semanticscholar +1 more source
Speed fluctuations of bacterial replisomes [PDF]
, 2021 Abstract Replisomes are multi-protein complexes that replicate genomes with remarkable speed and accuracy. Despite their importance, their dynamics is poorly characterized, especially in vivo. In this paper, we present an approach to infer the replisome dynamics from the DNA abundance distribution measured in a ...
Deepak Bhat +4 more
openaire +1 more source
Frequent exchange of the DNA polymerase during bacterial chromosome replication
eLife, 2017 The replisome is a multiprotein machine that carries out DNA replication. In Escherichia coli, a single pair of replisomes is responsible for duplicating the entire 4.6 Mbp circular chromosome. In vitro studies of reconstituted E.
Thomas R Beattie +6 more
doaj +1 more source
The extent of error-prone replication-restart by homologous recombination is controlled by Exo1 and checkpoint proteins [PDF]
, 2014 Genetic instability, a hallmark of cancer, can occur when the replication machinery encounters a barrier. The intra-S phase checkpoint maintains stalled replication forks in a replication-competent configuration by phosphorylating replisome components ...
Ahn +80 more
core +2 more sources
EMBO Journal, 2021 The eukaryotic replisome is rapidly disassembled during DNA replication termination. In metazoa, the cullin‐RING ubiquitin ligase CUL‐2LRR‐1 drives ubiquitylation of the CMG helicase, leading to replisome disassembly by the p97/CDC‐48 “unfoldase”.
Yisui Xia +4 more
semanticscholar +1 more source
Attaching Accessory Devices to the Replisome [PDF]
Molecular Cell, 2016 Evidence mounts, via two studies published in Molecular Cell (Villa et al. 2016; Samora et al. 2016), that Ctf4 recruits to the replisome various factors that play diverse roles in chromosome duplication, by acting as an interaction hub.
Takashi, Sutani, Katsuhiko, Shirahige
openaire +2 more sources
eLife, 2020 Sister chromatid cohesion essential for mitotic chromosome segregation is thought to involve the co-entrapment of sister DNAs within cohesin rings. Although cohesin can load onto chromosomes throughout the cell cycle, it only builds cohesion during S ...
Madhusudhan Srinivasan +4 more
doaj +1 more source
Millisecond single-molecule localization microscopy combined with convolution analysis and automated image segmentation to determine protein concentrations in complexly structured, functional cells, one cell at a time [PDF]
, 2015 We present a single-molecule tool called the CoPro (Concentration of Proteins) method that uses millisecond imaging with convolution analysis, automated image segmentation and super-resolution localization microscopy to generate robust estimates for ...
Leake, Mark C., Wollman, Adam J. M.
core +2 more sources
The eukaryotic replisome tolerates leading‐strand base damage by replicase switching
EMBO Journal, 2021 The high‐fidelity replicative DNA polymerases, Pol ε and Pol δ, are generally thought to be poorly equipped to replicate damaged DNA. Direct and complete replication of a damaged template therefore typically requires the activity of low‐fidelity ...
Thomas A. Guilliam, Joseph T. P. Yeeles
semanticscholar +1 more source
Mcm10 interacts with Rad4/Cut5(TopBP1) and its association with origins of DNA replication is dependent on Rad4/Cut5(TopBP1). [PDF]
, 2011 © Elsevier, 2011. This is a post-print version of the article. The definitive version is available from: http://www.sciencedirect.com/science/article/pii/S1568786411002369. Deposited in accordance with SHERPA RoMEO guidelinesInitiation of DNA replication
Aves, Stephen +4 more
core +1 more source