Results 21 to 30 of about 7,567,038 (395)

A reporter for noninvasively monitoring gene expression and plant transformation

open access: yesHorticulture Research, 2020
Reporters have been widely used to visualize gene expression, protein localization, and other cellular activities, but the commonly used reporters require special equipment, expensive chemicals, or invasive treatments.
Yubing He   +4 more
semanticscholar   +1 more source

Reporter Gene Assays [PDF]

open access: yes, 2009
Reporter gene assays are versatile and sensitive methods of assaying numerous targets in high-throughput drug-screening programs. A variety of reporter genes allow users a choice of signal that can be tailored to the required sensitivity, the available detection apparatus, the cellular system employed, and the required compatibility with multiplexed ...
Liu, A.M.F.   +3 more
openaire   +3 more sources

The protein kinase FvRIPK1 regulates plant morphogenesis by ABA signaling using seed genetic transformation in strawberry

open access: yesFrontiers in Plant Science, 2022
A strawberry RIPK1, a leu-rich repeat receptor-like protein kinase, is previously demonstrated to be involved in fruit ripening as a positive regulator; however, its role in vegetable growth remains unknown.
Xuexue Chen   +5 more
doaj   +1 more source

Massively parallel reporter assays of melanoma risk variants identify MX2 as a gene promoting melanoma

open access: yesNature Communications, 2020
Genome-wide association studies (GWAS) have identified ~20 melanoma susceptibility loci, most of which are not functionally characterized. Here we report an approach integrating massively-parallel reporter assays (MPRA) with cell-type-specific epigenome ...
Jiyeon Choi   +26 more
semanticscholar   +1 more source

In vitro and in vivo expression of foreign genes by transmissible gastroenteritis coronavirus-derived minigenomes [PDF]

open access: yes, 2002
A helper-dependent expression system based on transmissible gastroenteritis coronavirus (TGEV) has been developed using a minigenome of 3·9 kb (M39).
Alonso, S.   +8 more
core   +3 more sources

Establishment of a mouse embryonic stem cell line carrying a reporter of mT-F2A-EGFP based on CRISPR/Cas9n technology

open access: yesShanghai Jiaotong Daxue xuebao. Yixue ban, 2023
Objective·To establish a T-box transcription factor Brachyury (T gene) fluorescence reporter cell line, in which foot-and-mouth disease virus 2A (F2A) and enhanced green fluorescent protein (EGFP) were knocked in at the end of mouse T gene (mT-F2A-EGFP ...
WANG Jingyi, WANG Qiong
doaj   +1 more source

mCherry contains a fluorescent protein isoform that interferes with its reporter function

open access: yesFrontiers in Bioengineering and Biotechnology, 2022
Fluorescent proteins are essential reporters in cell and molecular biology. Here, we found that red-fluorescent proteins possess an alternative translation initiation site that produces a short functional protein isoform in both prokaryotes and ...
Maxime Fages-Lartaud   +5 more
doaj   +1 more source

A key role for leukemia inhibitory factor in C26 cancer cachexia [PDF]

open access: yes, 2015
Cachexia is an exacerbating event in many types of cancer that is strongly associated with a poor prognosis. We have identified cytokine, signaling, and transcription factors that are required for cachexia in the mouse C26 colon carcinoma model of cancer.
Jackman, Robert W.   +2 more
core   +1 more source

Drosophila melanogaster White Mutant w1118 Undergo Retinal Degeneration

open access: yesFrontiers in Neuroscience, 2018
Key scientific discoveries have resulted from genetic studies of Drosophila melanogaster, using a multitude of transgenic fly strains, the majority of which are constructed in a genetic background containing mutations in the white gene.
María José Ferreiro   +8 more
doaj   +1 more source

Positron emission tomography reporter gene strategy for use in the central nervous system

open access: yesProceedings of the National Academy of Sciences of the United States of America, 2019
Significance With any new treatment it is imperative the medical community has a way to monitor its effectiveness, tracking its location, monitoring its expression, and observing off-target effects. Gene/cell therapy is no exception.
Tom Haywood   +6 more
semanticscholar   +1 more source

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