Results 41 to 50 of about 7,873,006 (309)

Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus

BMC Developmental Biology, 2001
BackgroundSeveral Cre reporter strains of mice have been described, in which a lacZ gene is turned on in cells expressing Cre recombinase, as well as their daughter cells, following Cre-mediated excision of a loxP-flanked transcriptional "stop" sequence.
S. Srinivas, Tomoko Watanabe, Chyuan-Sheng Lin, C. William, Y. Tanabe, T. Jessell, F. Costantini   +6 more
semanticscholar   +1 more source

A Novel MitoTimer Reporter Gene for Mitochondrial Content, Structure, Stress, and Damage in Vivo*

Journal of Biological Chemistry, 2014
Background: Mitochondrial health is difficult to assess in vivo. Results: We have generated a reporter gene, MitoTimer, which targets mitochondria, and fluoresces green and shifts to red when oxidized, for assessment of mitochondrial content, structure ...
R. Laker, Peng Xu, K. Ryall, A. Sujkowski, Brandon M. Kenwood, Kristopher H Chain, Mei Zhang, M. Royal, Kyle L. Hoehn, M. Driscoll, P. Adler, R. Wessells, J. Saucerman, Zhen Yan   +13 more
semanticscholar   +1 more source

The Human Sodium Iodide Symporter as a Reporter Gene for Studying Middle East Respiratory Syndrome Coronavirus Pathogenesis

mSphere, 2018
Single photon emission computed tomography (SPECT) is frequently used in oncology and cardiology to evaluate disease progression and/or treatment efficacy.
Svetlana Chefer, Jurgen Seidel, Adam S. Cockrell, Boyd Yount, Jeffrey Solomon, Katie R. Hagen, David X. Liu, Louis M. Huzella, Mia R. Kumar, Elena Postnikova, J. Kyle Bohannon, Matthew G. Lackemeyer, Kurt Cooper, Ariel Endlich-Frazier, Heema Sharma, David Thomasson, Christopher Bartos, Philip J. Sayre, Amy Sims, Julie Dyall, Michael R. Holbrook, Peter B. Jahrling, Ralph S. Baric, Reed F. Johnson   +23 more
doaj   +1 more source

Specific Bacterial Pathogen Phytosensing Is Enabled by a Synthetic Promoter-Transcription Factor System in Potato

Frontiers in Plant Science, 2022
Phytosensors are genetically engineered plant-based sensors that feature synthetic promoters fused to reporter genes to sense and report the presence of specific biotic and abiotic stressors on plants.
Ramona Persad-Russell, Ramona Persad-Russell, Mitra Mazarei, Mitra Mazarei, Tayler Marie Schimel, Tayler Marie Schimel, Lana Howe, Lana Howe, Manuel J. Schmid, Manuel J. Schmid, Tayebeh Kakeshpour, Tayebeh Kakeshpour, Caitlin N. Barnes, Caitlin N. Barnes, Holly Brabazon, Holly Brabazon, Erin M. Seaberry, Erin M. Seaberry, D. Nikki Reuter, D. Nikki Reuter, Scott C. Lenaghan, Scott C. Lenaghan, C. Neal Stewart, C. Neal Stewart   +23 more
doaj   +1 more source

A Sensitive Sensor Cell Line for the Detection of Oxidative Stress Responses in Cultured Human Keratinocytes

Sensors, 2014
In the progress of allergic and irritant contact dermatitis, chemicals that cause the generation of reactive oxygen species trigger a heat shock response in keratinocytes.
Ute Hofmann, Melanie Priem, Christine Bartzsch, Thomas Winckler, Karl-Heinz Feller   +4 more
doaj   +1 more source

Expanding the genetic toolbox for the obligate human pathogen Streptococcus pyogenes

Frontiers in Bioengineering and Biotechnology
Genetic tools form the basis for the study of molecular mechanisms. Despite many recent advances in the field of genetic engineering in bacteria, genetic toolsets remain scarce for non-model organisms, such as the obligatory human pathogen Streptococcus ...
Nina Lautenschläger, Katja Schmidt, Carolin Schiffer, Thomas F. Wulff, Karin Hahnke, Knut Finstermeier, Moïse Mansour, Alexander K. W. Elsholz, Emmanuelle Charpentier, Emmanuelle Charpentier   +9 more
doaj   +1 more source

Generation of Epichloë Strains Expressing Fluorescent Proteins Suitable for Studying Host-Endophyte Interactions and Characterisation of a T-DNA Integration Event

Microorganisms, 2019
Methods for the identification and localisation of endophytic fungi are required to study the establishment, development, and progression of host-symbiont interactions, as visible reactions or disease symptoms are generally absent from host plants ...
Inoka K. Hettiarachchige, Emma J. Ludlow, Piyumi N. Ekanayake, Natasha D. Brohier, Sareena Sahab, Timothy I. Sawbridge, German C. Spangenberg, Kathryn M. Guthridge   +7 more
doaj   +1 more source

Evaluating the MicroRNA Targeting Sites by Luciferase Reporter Gene Assay

Methods in molecular biology, 2013
MicroRNAs are post-transcriptional regulators that control mRNA stability and the translation efficiency of their target genes. Mature microRNAs are approximately 22-nucleotide in length.
Yi Jin, Zujian Chen, Xiqiang Liu, Xiaofeng Zhou   +3 more
semanticscholar   +1 more source

Development of Zika Virus Mini-Replicon Based Single-Round Infectious Particles as Gene Delivery Vehicles

Viruses, 2023
Zika virus (ZIKV) is a type of RNA virus that belongs to the Flaviviridae family. We have reported the construction of a DNA-launched replicon of the Asian-lineage Natal RGN strain and the production of single-round infectious particles (SRIPs) via the ...
Joh-Sin Wu, Ju-Ying Kan, Hsueh-Chou Lai, Cheng-Wen Lin   +3 more
doaj   +1 more source

Tyrosinase as a multifunctional reporter gene for Photoacoustic/MRI/PET triple modality molecular imaging

Scientific Reports, 2013
Development of reporter genes for multimodality molecular imaging is highly important. In contrast to the conventional strategies which have focused on fusing several reporter genes together to serve as multimodal reporters, human tyrosinase (TYR) – the ...
C. Qin, K. Cheng, Kai Chen, Xiang Hu, Yang Liu, X. Lan, Yongxue Zhang, Hongguang Liu, Yingding Xu, L. Bu, X. Su, Xiaohua Zhu, Shuxian Meng, Zhen Cheng   +13 more
semanticscholar   +1 more source