Molecular Imaging, 2005 The human and rodent sodium iodide symporters ( NIS ) have recently been cloned and are being investigated as potential therapeutic and reporter genes. We have extended this effort by constructing an internal ribosomal entry site (IRES)-linked human NIS (
Jiantu Che +5 more
doaj +1 more source
CAR Chase: Where Do Engineered Cells Go in Humans?
Frontiers in Oncology, 2020 Chimeric antigen receptor (CAR) – and T-cell receptor (TCR) – modified T-cells are rapidly emerging as a viable treatment option for cancer patients.
Simone Krebs +7 more
doaj +1 more source
Reporter constructs with low background activity utilizing the cat gene [PDF]
, 1992 Reporter plasmids utilizing the cat gene for the analysis of promoter and enhancer sequences in vertebrate cells, were constructed. These plasmids minimize the background of transcription derived from cryptic promoters or cryptic regulatory elements ...
Andrea Schmidt +11 more
core +1 more source
Cryptic transcripts from a ubiquitous plasmid origin of replication confound tests for cis-regulatory function. [PDF]
, 2012 A vast amount of research on the regulation of gene expression has relied on plasmid reporter assays. In this study, we show that plasmids widely used for this purpose constitutively produce substantial amounts of RNA from a TATA-containing cryptic ...
Hiraoka, Kei +3 more
core +2 more sources
Effective gene expression prediction from sequence by integrating long-range interactions
Nature Methods, 2021 How noncoding DNA determines gene expression in different cell types is a major unsolved problem, and critical downstream applications in human genetics depend on improved solutions.
Žiga Avsec +9 more
semanticscholar +1 more source
Comparison of Short-Term Estrogenicity Tests for Identification of Hormone-Disrupting Chemicals [PDF]
, 1999 The aim of this study was to compare results obtained by eight different short-term assays of estrogenlike actions of chemicals conducted in 10 different laboratories in five countries. Twenty chemicals were selected to represent direct-acting estrogens,
Ana M. Soto +34 more
core +2 more sources
Multimodality reporter gene imaging: Construction strategies and application
Theranostics, 2018 Molecular imaging has played an important role in the noninvasive exploration of multiple biological processes. Reporter gene imaging is a key part of molecular imaging.
Mengting Li +3 more
semanticscholar +1 more source
The HSV-1 Latency-Associated Transcript Functions to Repress Latent Phase Lytic Gene Expression and Suppress Virus Reactivation from Latently Infected Neurons [PDF]
, 2016 open access articleHerpes simplex virus 1 (HSV-1) establishes life-long latent infection within sensory neurons, during which viral lytic gene expression is silenced.
Coleman, Heather M. +7 more
core +3 more sources
Labeling Ebola Virus with a Self-Splicing Fluorescent Reporter
Microorganisms, 2022 Inteins (intervening proteins) are polypeptides that interrupt the sequence of other proteins and remove themselves through protein splicing. In this intein-catalyzed reaction, the two peptide bonds surrounding the intein are rearranged to release the ...
Baylee Heiden +3 more
doaj +1 more source
CRISPR-Cas9-mediated functional dissection of 3'-UTRs. [PDF]
, 2017 Many studies using reporter assays have demonstrated that 3' untranslated regions (3'-UTRs) regulate gene expression by controlling mRNA stability and translation.
Ahituv, Nadav +6 more
core +1 more source