Results 51 to 60 of about 7,567,038 (395)

Evaluation of beta-galactosidase activity in tissue in the presence of blood [PDF]

open access: yes, 2000
The reporter gene for beta -galactosidase is frequently used to determine the efficiency of gene transfer in arteries. However, blood is often present in arterial explants and may compromise the results by the presence of hemoglobin. The light absorption
Armeanu, Sorin   +2 more
core   +1 more source

Multi-wavelength photoacoustic imaging of inducible tyrosinase reporter gene expression in xenograft tumors

open access: yesScientific Reports, 2014
Photoacoustic imaging is an emerging hybrid imaging technology capable of breaking through resolution limits of pure optical imaging technologies imposed by optical-scattering to provide fine-resolution optical contrast information in deep tissues.
R. Paproski   +3 more
semanticscholar   +1 more source

Imaging Molecular Pathways: Reporter Genes [PDF]

open access: yesRadiation Research, 2012
Molecular imaging is a rapidly advancing field that allows cancer biologists to look deeper into the complex inner workings of tumor cells, or whole tumors, in a non-invasive manner. In this review, we will summarize some recent advances that enable investigators to study various important biological processes in tumors in vivo.
John, Brogan   +5 more
openaire   +2 more sources

Expanding the genetic toolbox for the obligate human pathogen Streptococcus pyogenes

open access: yesFrontiers in Bioengineering and Biotechnology
Genetic tools form the basis for the study of molecular mechanisms. Despite many recent advances in the field of genetic engineering in bacteria, genetic toolsets remain scarce for non-model organisms, such as the obligatory human pathogen Streptococcus ...
Nina Lautenschläger   +9 more
doaj   +1 more source

Generation of Epichloë Strains Expressing Fluorescent Proteins Suitable for Studying Host-Endophyte Interactions and Characterisation of a T-DNA Integration Event

open access: yesMicroorganisms, 2019
Methods for the identification and localisation of endophytic fungi are required to study the establishment, development, and progression of host-symbiont interactions, as visible reactions or disease symptoms are generally absent from host plants ...
Inoka K. Hettiarachchige   +7 more
doaj   +1 more source

Development of Zika Virus Mini-Replicon Based Single-Round Infectious Particles as Gene Delivery Vehicles

open access: yesViruses, 2023
Zika virus (ZIKV) is a type of RNA virus that belongs to the Flaviviridae family. We have reported the construction of a DNA-launched replicon of the Asian-lineage Natal RGN strain and the production of single-round infectious particles (SRIPs) via the ...
Joh-Sin Wu   +3 more
doaj   +1 more source

Coupling of adenovirus to transferrin-polylysine/DNA complexes greatly enhances receptor-mediated gene delivery and expression of transfected genes. [PDF]

open access: yes, 1992
We are developing efficient methods for gene transfer into tissue culture cells. We have previously shown that coupling of a chimeric adenovirus with polylysine allowed the construction of an adenovirus-polylysine-reporter-gene complex that transferred ...
Birnstiel, Max L.   +6 more
core   +1 more source

Tyrosinase as a multifunctional reporter gene for Photoacoustic/MRI/PET triple modality molecular imaging

open access: yesScientific Reports, 2013
Development of reporter genes for multimodality molecular imaging is highly important. In contrast to the conventional strategies which have focused on fusing several reporter genes together to serve as multimodal reporters, human tyrosinase (TYR) – the ...
C. Qin   +13 more
semanticscholar   +1 more source

Highly Sensitive Luminescent Bioassay Using Recombinant Escherichia coli Biosensor for Rapid Detection of Low Cr(VI) Concentration in Environmental Water

open access: yesBiosensors, 2021
In this study, we constructed a recombinant Escherichia coli strain with different promoters inserted between the chromate-sensing regulator chrB and the reporter gene luxAB to sense low hexavalent chromium (Cr(VI)) concentrations (
Guey-Horng Wang   +4 more
doaj   +1 more source

Green Fluorescent Protein (GFP) in Vector Systems Played Sense Role of Epigenetic in Plants [PDF]

open access: yes, 2008
The green fluorescent protein (GFP) of jellyfish (_Aequorea victoria_) has significant advantages over other reporter genes, because expression can be detected in living cells without any substrates.
Hany A. El-Shemy   +2 more
core   +1 more source

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