Results 91 to 100 of about 416,000 (310)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

Financial Repression, Bank Deposits, Real Assets and Black Money [PDF]

open access: yes
Consider real assets and bank deposits. If returns on deposits improve due to reduction in financial repression, then investment in real assets can fall.
Gurbachan Singh
core  

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

Tax evasion and financial repression: A reconsideration using endogenous growth models [PDF]

open access: yes
Using two dynamic monetary general equilibrium models characterized by endogenous growth, financial repression and endogenously determined tax evasion, we analyze whether financial repression can be explained by tax evasion.
Emmanuel Ziramba, Rangan Gupta
core  

Hidden Repression Under Dictatorships [PDF]

open access: yes, 2014
This paper presents a model of the interactions between a dictator and a mass of citizens in which the dictator might have an incentive to use hidden repression. Most papers on political economy of dictatorships assume that the role of repression might
Moreno Medina, Jonathan
core  

Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus

open access: yesFEBS Open Bio, EarlyView.
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima   +7 more
wiley   +1 more source

Digital Repression Index 2010-19

open access: yes, 2020
The data describes global patterns of digital repression from 2010-19 and incorporates two composite indexes: a latent construct of digital repression, and a latent construct of digital repression capacity.
Feldstein, S (via Mendeley Data)
core   +1 more source

The Impact of Pro-Government Militias on Human Rights Violations [PDF]

open access: yes, 2014
New data show that between 1982 and 2007, in over 60 countries governments were linked to and cooperated with informal armed groups within their own borders.
Mitchell, NJ   +5 more
core   +1 more source

Heterotropic regulation and negative homotropic cooperativity

open access: yesFEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea   +5 more
wiley   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

open access: yesFEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain   +11 more
wiley   +1 more source

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