Results 111 to 120 of about 334,121 (353)

Isolation of the gal Repressor [PDF]

Proceedings of the National Academy of Sciences, 1971
The repressor of the galactose operon of Escherichia coli has been partially purified and identified as a protein. Induction of a lysogen in which λ was linked to the bacterial gal R and lysine genes resulted in a large increase in the production of the gal repressor ...
Robert A. Weisberg, Michael M. Gottesman, J. S. Parks, Ira Pastan, Robert L. Perlman, K. Shimada   +5 more
openaire   +3 more sources

Targeting the MDM2‐MDM4 interaction interface reveals an otherwise therapeutically active wild‐type p53 in colorectal cancer

Molecular Oncology, EarlyView.
This study investigates an alternative approach to reactivating the oncosuppressor p53 in cancer. A short peptide targeting the association of the two p53 inhibitors, MDM2 and MDM4, induces an otherwise therapeutically active p53 with unique features that promote cell death and potentially reduce toxicity towards proliferating nontumor cells.
Sonia Valentini, Giada Mele, Marika Attili, Maria Rita Assenza, Fulvio Saccoccia, Francesca Sardina, Cinzia Rinaldo, Roberto Massari, Nicola Tirelli, Alfredo Pontecorvi, Fabiola Moretti   +10 more
wiley   +1 more source

Effect of supercoiling on formation of protein mediated DNA loops [PDF]

, 2006
DNA loop formation is one of several mechanisms used by organisms to regulate genes. The free energy of forming a loop is an important factor in determining whether the associated gene is switched on or off. In this paper we use an elastic rod model of DNA to determine the free energy of forming short (50--100 basepair), protein mediated DNA loops ...
arxiv   +1 more source

The Energetics of Molecular Adaptation in Transcriptional Regulation [PDF]

, 2019
Mutation is a critical mechanism by which evolution explores the functional landscape of proteins. Despite our ability to experimentally inflict mutations at will, it remains difficult to link sequence-level perturbations to systems-level responses. Here,
Barnes, Stephanie L., Belliveau, Nathan M., Chure, Griffin, Einav, Tal, Kaczmarek, Zofii, Lewis, Mitchell, Phillips, Rob, Razo-Mejia, Manuel   +7 more
core   +1 more source

The atypical KRASQ22K mutation directs TGF‐β response towards partial epithelial‐to‐mesenchymal transition in patient‐derived colorectal cancer tumoroids

Molecular Oncology, EarlyView.
TGF‐β has a complex role in cancer, exhibiting both tumor‐suppressive and tumor‐promoting properties. Using a series of differentiated tumoroids, derived from different stages and mutational background of colorectal cancer patients, we replicate this duality of TGF‐β in vitro. Notably, the atypical but highly aggressive KRASQ22K mutation rendered early‐
Theresia Mair, Philip König, Milena Mijović, Jessica Kalla, Anil Baskan, Loan Tran, Kristina Draganić, Pedro Morata Saldaña, Carlos Uziel Pérez Malla, Janette Pfneissl, Andreas Tiefenbacher, Julijan Kabiljo, Velina S. Atanasova, Lisa Wozelka‐Oltjan, Leonhard Müllauer, Michael Bergmann, Raheleh Sheibani‐Tezerji, Gerda Egger   +17 more
wiley   +1 more source

A 314-bp SINE insertion in the ZNF2 promoter region may act as a repressor related to regulation of fat deposition in pigs

Journal of Integrative Agriculture, 2023
Retrotransposons, a type of DNA fragment that can mobilize itself on genome, can generate genetic variations and develop for molecular markers based on the insertion polymorphism.
Hao GU, Zhan-yu DU, Eduard MURANI, Enrico D'ALESSANDRO, Cai CHEN, Xiao-yan WANG, Jiu-de MAO, Klaus WIMMERS, Cheng-yi SONG   +8 more
doaj  

Construction and utilisation of a bidirectional reporter vector in the analysis of two nod-boxes in of Rhizobium loti : a thesis presented in partial fulfilment of the requirements for the Degree of Master of Science in Molecular Genetics at Massey University [PDF]

, 1993
The nod-box is a 47bp cis-acting regulatory region which has been conserved amongst every species of Rhizobium studied to date. In species such as R. meliloti and R.
Parry, Simon Keith
core  

Designer Gene Networks: Towards Fundamental Cellular Control

, 2001
The engineered control of cellular function through the design of synthetic genetic networks is becoming plausible. Here we show how a naturally occurring network can be used as a parts list for artificial network design, and how model formulation leads ...
Ackers, Arkin, Barkai, Becksei, Bialek, Bray, Chen, Dickson, Elowitz, Endy, Gardner, Gillespie, Glass, Glass, Glass, Hammond, Harada, Harding, Hartwell, Hasty, Jacob, Johnson, Johnson, Kauffman, Keller, Lauffenburger, Lowman, McAdams, McAdams, McAdams, McClure, Meyer, Monod, Monod, Moran, Novak, Novak, Novick, Ohlendorf, Palsson, Ptaschne, Reinitz, Rozanov, Samoilov, Sancho, Savageau, Savageau, Savageau, Shea, Smolen, Sveiczer, Szafranski, Tyson, Villaverde, von Hippel, Wolf, Wong   +56 more
core   +1 more source

The Role of Repression in the Incidence of Ironic Errors [PDF]

, 2008
The role of repression in the incidence of ironic errors was investigated on a golf task. Coping styles of novice golfers were determined using measures of cognitive anxiety and physiological arousal.
Davis, Paul, Woodman, Tim
core   +1 more source

Thecl repressor of bacteriophage P1 operator-repressor interaction of wild-type and mutant repressor proteins

Nucleic Acids Research, 1989
The c1 repressor gene of bacteriophage P1 and the temperature-sensitive mutants P1c1.100 and P1c1.162 was cloned into an expression vector and the repressor proteins were overproduced. A rapid purification procedure was required for the isolation of the thermolabile repressor proteins.
Hans Dieter Riedel, Heinz Suhuster, Barbara R. Baumstark, Makoto Kimura, Jochen Hemrich   +4 more
openaire   +3 more sources