Results 101 to 110 of about 264,694 (307)

Ionomycin suppresses cancer cell growth by disrupting mitochondrial transcription

FEBS Open Bio, EarlyView.
In this study, we identify a new function for the selective Ca2+ ionophore, ionomycin, as an inhibitor of mitochondrial transcription. Both total and nascent RNA analyses revealed that ionomycin treatment reduces the transcription of mitochondrial genes.
Lishen Wang, Mingqiang Deng, Dongmei He, Diqin Lai, Dongjun Liu, Dengyong Zhu, Zhenhua Zhang, Peng Tang, Chuanman Zhou, Menghui Yin, Xichen Bao   +10 more
wiley   +1 more source

An enhanced CRISPR repressor for targeted mammalian gene regulation [PDF]

, 2018
The RNA-guided endonuclease Cas9 can be converted into a programmable transcriptional repressor, but inefficiencies in target-gene silencing have limited its utility.
Alissa Lance-Byrne, Ryan J. Cecchi, Swechchha Pradhan, Yeo, Nan Cher, Cecchi, Ryan J., Xiaoge Guo, Nathan E. Lewis, Sharma, Sumana, Davidsohn, Noah, Collins, James J, Angela Tung, Pradhan, Swechchha, Marcelle Tuttle, Kuo, Chih-Chung, Nan Cher Yeo, Noah Davidsohn, Alejandro Chavez, Ebrahimkhani, Mo R, Chan, Yingleong, Ebrahimkhani, Mo R., Elaine T. Lim, Sumana Sharma, Collins, James J., Cecchi, Ryan J, Lim, Elaine T, Church, George M, Denitsa Milanova, Kiani, Samira, Chih-Chung Kuo, Tung, Angela, Lewis, Nathan E., George M. Church, Lance-Byrne, Alissa, Chavez, Alejandro, Menn, David, Tuttle, Marcelle, Yingleong Chan, James J. Collins, David Menn, Mo R. Ebrahimkhani, Samira Kiani, Lewis, Nathan E, Milanova, Denitsa, Guo, Xiaoge, Church, George M., Lim, Elaine T.   +45 more
core   +1 more source

Repressor activator protein 1–promoted colorectal cell migration is associated with the regulation of Vimentin

Tumor Biology, 2017
Repressor activator protein 1 plays important roles in telomere protection, while repressor activator protein 1 binds to extra-telomeric DNA and exerts the function as a transcriptional regulator.
Yongyong Yang, Chunxiang Ye, Lixin Wang, Guo An, Zhihua Tian, Lin Meng, Like Qu, Shenyi Lian, Chengchao Shou   +8 more
doaj   +1 more source

Identification of (20R)‐protopanaxadiol from Panax ginseng as a novel anti‐SARS‐CoV‐2 compound

FEBS Open Bio, EarlyView.
We established a noninfectious BAC‐based SARS‐CoV‐2 replicon that enables antiviral screening under BSL‐2 conditions. Using this platform, we screened 373 food‐derived compounds and identified (20R)‐protopanaxadiol from Panax ginseng as a novel inhibitor of SARS‐CoV‐2 RNA replication, highlighting the value of safe replicon systems for antiviral ...
Midori Takeda, Natsue Maezono, Rina Uchikoshi, Nobuyuki Kato, Masanori Ikeda   +4 more
wiley   +1 more source

Dimer−Dimer Interfaces of the λ-Repressor Are Different in Liganded and Free States

, 1996
λ-Repressor dimers associate in solution to form tetramers and higher order structures. Dimer-dimer contact is also crucial in cooperative binding to adjacent operators.
MUKHOPADHYAY, Chhanda, ROY, Siddhartha, BANDYOPADHYAY, Sumita, Sumita Bandyopadhyay, Siddhartha Roy, Chhanda Mukhopadhyay   +5 more
core   +1 more source

Protein-induced changes in DNA structure and dynamics observed with noncovalent site-directed spin-labeling and PELDOR [PDF]

, 2012
Site-directed spin labeling and pulsed electron–electron double resonance (PELDOR or DEER) have previously been applied successfully to study the structure and dynamics of nucleic acids. Spin labeling nucleic acids at specific sites requires the covalent
Reginsson, Gunnar Widtfeldt, Rouillon, Christophe, Schiemann, Olav, Sigurdsson, Snorri T, White, Malcolm F, Shelke, Sandip, White, Malcolm F; id_orcid   +6 more
core   +1 more source

Short form of weinberger adjustment inventory (WAI): psychometric properties and confirmatory factor analysis of the Persian version

مجله علوم روانشناختی, 2016
Excessive inhibition of emotions is associated with a variety of psychological and health problems. Weinberger developed an inventory to assess socio-emotional adjustment.
Zoha Saeedi, Nima Ghorbani, MohammadReza Sarafraz   +2 more
doaj  

Two-Hybrid System for Characterization of Protein-Protein Interactions in E. coli

BioTechniques, 2000
The yeast two-hybrid system has been used to characterize many protein-protein interactions. A two-hybrid system for E. coli was constructed in which one hybrid protein bound to a specific DNA site recruits another to an adjacent DNA binding site.
Lori B. Hays, Yuen-Shing A. Chen, James C. Hu   +2 more
doaj   +1 more source

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

Recognition of DNA structure by 434 repressor

, 1998
In complexes of bacteriophage 434 binding sites with 434 repressor the central 4 bp of the 14 bp site are not contacted by the protein, although changes in these bases alter binding site affinity for the repressor.
Gerald B. Koudelka
core