Results 111 to 120 of about 264,694 (307)
Repressor and Activator Dynamics
(A and B) Shows repressor (y), activator (x) phase plane analysis for both designs. Nullclines and limit cycle trajectory (red line) close to the bifurcation point.
Juan F Poyatos (6406) +1 more
core +1 more source
Determinants of bacteriophage 933W repressor DNA binding specificity.
We reported previously that 933W repressor apparently does not cooperatively bind to adjacent sites on DNA and that the relative affinities of 933W repressor for its operators differ significantly from that of any other lambdoid bacteriophage.
Tammy J Bullwinkle +3 more
doaj +1 more source
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
An illustrations of the Lac repressor in its possible conformations.
A space filling model of the Lac repressor tetramer in its stiff V-shaped conformation (right) and a schematic representation of our assumed model of the open (extended) conformation of the Lac repressor (left).
David Swigon (282297) +3 more
core +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Heilmann H, Reeve JN, Pühler A. Identification of the repressor and repressor bypass (antirepressor) polypeptides of bacteriophage P1 synthesized in infected minicells. Mol Gen Genet.
Heilmann, Heidrun +2 more
core +1 more source
Background Lysogenic mode of life cycle of a temperate bacteriophage is generally maintained by a protein called 'repressor'. Repressor proteins of temperate lambdoid phages bind to a few symmetric operator DNAs in order to regulate their gene expression.
Mandal Nitai C +6 more
doaj +1 more source
Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima +7 more
wiley +1 more source
Multiple repressor binding sites in the genome of bacteriophage P1 [PDF]
Velleman M, Dreiseikelmann B, Schuster H. Multiple repressor binding sites in the genome of bacteriophage P1. Proceedings of the National Academy of Sciences of the United States of America.
Velleman, Mathias +2 more
core
Evi-1 transforming and repressor activities are mediated by CtBP co-repressor proteins [PDF]
Ectopic production of the EVI1 transcriptional repressor zinc finger protein is seen in 4--6% of human acute myeloid leukemias. Overexpression also transforms Rat1 fibroblasts by an unknown mechanism, which is likely to be related to its role in leukemia
Walker, Mark +6 more
core +1 more source

