Results 81 to 90 of about 355,752 (283)

Silencing cryptic specialized metabolism in Streptomyces by the nucleoid-associated protein Lsr2

eLife, 2019
Lsr2 is a nucleoid-associated protein conserved throughout the actinobacteria, including the antibiotic-producing Streptomyces. Streptomyces species encode paralogous Lsr2 proteins (Lsr2 and Lsr2-like, or LsrL), and we show here that of the two, Lsr2 has
Emma J Gehrke, Xiafei Zhang, Sheila M Pimentel-Elardo, Andrew R Johnson, Christiaan A Rees, Stephanie E Jones, Hindra, Sebastian S Gehrke, Sonya Turvey, Suzanne Boursalie, Jane E Hill, Erin E Carlson, Justin R Nodwell, Marie A Elliot   +13 more
doaj   +1 more source

Determination of ADP/ATP translocase isoform ratios in malignancy and cellular senescence

Molecular Oncology, EarlyView.
The individual functions of three isoforms exchanging ADP and ATP (ADP/ATP translocases; ANTs) on the mitochondrial membrane remain unclear. We developed a method for quantitatively differentiating highly similar human ANT1, ANT2, and ANT3 using parallel reaction monitoring. This method allowed us to assess changes in translocase levels during cellular
Zuzana Liblova, Dominika Maurencova, Barbora Salovska, Marek Kratky, Tomas Mracek, Zuzana Korandova, Alena Pecinova, Pavla Vasicova, David Rysanek, Ladislav Andera, Ivo Fabrik, Rudolf Kupcik, Pavel Kashmel, Pinky Sultana, Vojtech Tambor, Jiri Bartek, Josef Novak, Marie Vajrychova, Zdenek Hodny   +18 more
wiley   +1 more source

Noise-based switches and amplifiers for gene expression

, 2000
The regulation of cellular function is often controlled at the level of gene transcription. Such genetic regulation usually consists of interacting networks, whereby gene products from a single network can act to control their own expression or the ...
Asbury, Dickson, Gardner, Harada, Johnson, Johnson, Keller, Liu, McAdams, McAdams, Ptashne, von Hippel, Wolf, Xie, Yuh   +14 more
core   +2 more sources

A single aromatic residue in transcriptional repressor protein KorA is critical for cooperativity with its co-regulator KorB [PDF]

, 2008
A central feature of broad host range IncP-1 plasmids is the set of regulatory circuits that tightly control plasmid core functions under steady-state conditions.
Adamczyk, Bailey, Barnard, Bell, Bhattacharyya, Bignell, Bingle, Bingle, Bingle, Chahla, Chen, Chintakayala, Chiu, Delaglio, Dodd, Feng, Gornall, Grant, Greated, Greated, Jagura-Burdzy, Jagura-Burdzy, Jagura-Burdzy, Jagura-Burdzy, Jain, Jobichen, Kahn, Khare, Kostelidou, Kostelidou, Larsen, Lee, Ling, Lukaszewicz, Macartney, McKenney, Merika, Newberry, Niu, Pace, Pansegrau, Pedersen, Rajasekar, Rhodes, Sambrook, Schneiker, Sevostyanova, Shin, Smith, Studier, Thomas, Tuch, Valentine, Vranken, Wade, Watson, Whitty, Williams, Zatyka, Zukowski   +59 more
core   +2 more sources

The anticancer effect of the HDAC inhibitor belinostat is enhanced by inhibitors of Bcl‐xL or Mcl‐1 in ovarian cancer

Molecular Oncology, EarlyView.
The pan‐HDAC inhibitor belinostat increases the expression of the pro‐apoptotic proteins Bim, Puma, and Noxa and induces apoptosis in ovarian cancer cell lines and patient‐derived tumor organoids when used at high concentrations. Moreover, inhibiting the anti‐apoptotic proteins Bcl‐xL or Mcl‐1 sensitizes these preclinical models to the cytotoxic effect
Cécilia Thomine, Sterenn Guillemot, Louis‐Bastien Weiswald, Romane Florent, Edwige Abeilard, Florence Giffard, Emilie Brotin, Mélanie Briand, Enora Dolivet, Laurent Poulain, Marie Villedieu   +10 more
wiley   +1 more source

A nucleotide‐independent, pan‐RAS‐targeted DARPin elicits anti‐tumor activity in a multimodal manner

Molecular Oncology, EarlyView.
We report a Designed Ankyrin Repeat Protein that binds and inhibits RAS proteins, which serve as central cell signaling hubs and are essential for the progression of many cancers. Its unique feature is that it does not discriminate between different RAS isoforms or mutations and is capable of binding to RAS in both its active (GTP‐bound) and inactive ...
Jonas N. Kapp, Wouter P. R. Verdurmen, Jonas V. Schaefer, Kari Kopra, Gabriela Nagy‐Davidescu, Elodie Richard, Marie‐Julie Nokin, Patrick Ernst, Rastislav Tamaskovic, Martin Schwill, Ralph Degen, Claudia Scholl, David Santamaria, Andreas Plückthun   +13 more
wiley   +1 more source

Compositionality, stochasticity and cooperativity in dynamic models of gene regulation

, 2007
We present an approach for constructing dynamic models for the simulation of gene regulatory networks from simple computational elements. Each element is called a ``gene gate'' and defines an input/output-relationship corresponding to the binding and ...
Alberts B, Andrew Phillips, Blossey R, Bundschuh R, Cherry JL, De Jong H, Fall CP, Freidlin MI, Kim J, Lee Deville RE, Luca Cardelli, Milner R, Müller S, Phillips A, Priami C, Ralf Blossey, Smolen P, Thomas R, Thomas R, Van Kampen NG   +19 more
core   +2 more sources

Unraveling LINE‐1 retrotransposition in head and neck squamous cell carcinoma

Molecular Oncology, EarlyView.
The novel RetroTest method allows the detection of L1 activation in clinical samples with low DNA input, providing global L1 activity and the identification of the L1 source element. We applied RetroTest to a real‐world cohort of HNSCC patients where we reported an early L1 activation, with more than 60% of T1 patients showing L1 activity.
Jenifer Brea‐Iglesias, Ana Oitabén, Sonia Zumalave, Bernardo Rodriguez‐Martin, María Gallardo‐Gómez, Martín Santamarina, Ana Pequeño‐Valtierra, Laura Juaneda‐Magdalena, Ramón García‐Escudero, José Luis López‐Cedrún, Máximo Fraga, José M. C. Tubio, Mónica Martínez‐Fernández   +12 more
wiley   +1 more source

The evolution of Bordetella pertussis has selected for mutations of acr that lead to sensitivity to hydrophobic molecules and fatty acids

Emerging Microbes and Infections, 2019
Whooping cough, or pertussis, is resurgent in numerous countries worldwide. This has renewed interest in Bordetella pertussis biology and vaccinology. The in vitro growth of B. pertussis has been a source of difficulty, both for the study of the organism
Iain MacArthur, Thomas Belcher, Jerry D. King, Vasantha Ramasamy, Munirah Alhammadi, Andrew Preston   +5 more
doaj   +1 more source

BMP antagonist CHRDL2 enhances the cancer stem‐cell phenotype and increases chemotherapy resistance in colorectal cancer

Molecular Oncology, EarlyView.
Overexpression of CHRDL2 in colon cancer cells makes them more stem‐like and resistant to chemo‐ and radiotherapy. CHRDL2‐high cells have upregulation of the WNT pathway, genes involved in the DNA damage response (DDR) pathway and epithelial‐to‐mesenchymal transition (EMT). This leads to quicker repair of damaged DNA and more cell migration.
Eloise Clarkson, Annabelle Lewis
wiley   +1 more source