Results 231 to 240 of about 191,078 (267)
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Ultraviolet difference spectra of the lactose repressor protein
Biochimica et Biophysica Acta (BBA) - Protein Structure, 1973Abstract Ultraviolet difference spectra of the lactose repressor have been studied under various conditions. Changes in the aromatic residues of the protein upon addition of inducers, but not anti-inducers, are found. The extent of exposure of aromatic residues to solvent perturbation is also found to differ in the presence of inducers and anti ...
K S, Matthews +3 more
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Escherichia coli Repressor Proteins
1989Genetic regulation is an essential function in all living organisms. In prokaryotes genetic control provides responsivity to a constantly changing external milieu, and bacterial systems have proved extremely valuable in elucidating the variety of potential mechanisms.
Kyle L. Wick, Kathleen S. Matthews
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Immobilisation of a repressor protein for binding of plasmid DNA
Journal of Chromatography A, 2005The use of plasmid DNA in gene therapy and genetic vaccination has increased the need for scalable and sustainable production processes. One key challenge for bioprocess engineering is the separation of plasmid DNA from structurally related impurities. Affinity purification procedures allow a highly selective capturing of the target molecule.
Anja, Hasche, Carsten, Voss
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Lactose Repressor Protein: Functional Properties and Structure
1997The lactose repressor protein (LacI), the prototype for genetic regulatory proteins, controls expression of lactose metabolic genes by binding to its cognate operator sequences in E. coli DNA. Inducer binding elicits a conformational change that diminishes affinity for operator sequences with no effect on nonspecific binding. The release of operator is
K S, Matthews, J C, Nichols
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Biophysical Chemistry, 2007
Specific interactions between proteins and ligands that modify their functions are crucial in biology. Here, we examine sugars that bind the lactose repressor protein (LacI) and modify repressor affinity for operator DNA using isothermal titration calorimetry and equilibrium DNA binding experiments.
Corey J, Wilson +3 more
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Specific interactions between proteins and ligands that modify their functions are crucial in biology. Here, we examine sugars that bind the lactose repressor protein (LacI) and modify repressor affinity for operator DNA using isothermal titration calorimetry and equilibrium DNA binding experiments.
Corey J, Wilson +3 more
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Characterization and modification of a monomeric mutant of the lactose repressor protein
Biochemistry, 1986A monomeric mutant lactose repressor protein (T-41), containing serine at position 282 in place of tyrosine [Schmitz, A., Schmeissner, U., Miller, J. H., & Lu, P. (1976) J. Biol. Chem. 251, 3359-3366], has been purified by a series of chromatographic and precipitation methods.
T J, Daly, K S, Matthews
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The Trp Repressor, A Ligand-Activated Regulatory Protein
1992Publisher Summary This chapter discusses the role of Trp repressor as a ligand-activated regulatory protein. The Trp repressor (protein) of Escherichia coli is one of the most exhaustively studied DNA-binding regulatory macromolecules. Its physiological role is to reduce or eliminate transcription from certain regulatable promoters through the ...
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Molecular Characterization of the VIP Receptor Transcriptional Repressor Protein
Annals of the New York Academy of Sciences, 2000Abstract: The rat type 1 VIP receptor transcriptional repressor protein (VIPR‐RP) is a recently isolated novel transcription factor. In the study reported here, the functional domains of VIPR‐RP were characterized. To map the DNA binding domain, various regions of VIPR‐RP were either transcribed and translated in vitro or expressed in and purified ...
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Capsid protein of f2 as translational repressor
Biochemical and Biophysical Research Communications, 1967R, Ward, K, Shive, R, Valentine
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