Results 71 to 80 of about 471,185 (297)

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Enhancing sustainable innovation through workplace well-being and digital capability: A systematic literature review toward responsible and cleaner consumption

Cleaner and Responsible Consumption
This systematic literature review explores the junction of workplace well-being, digital capacity, and innovation performance in reaction to increasing calls for ethical innovation and sustainable business practices. With specific focus on Asia and China,
Qijun Li, Ilhaamie binti Abdul Ghani Azmi, Azah Anir binti Norman   +2 more
doaj   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Choosing clean: Do Indian consumers intend to purchase microplastic-free personal care products

Cleaner and Responsible Consumption
Plastic microbeads in personal care products have raised significant environmental concerns, prompting regulatory interventions worldwide. However, consumer intentions play a crucial role in ensuring the success of such initiatives, particularly in ...
Riya Kumbukattu Alex, Nimitha Aboobaker, Suja Purushothaman Devipriya   +2 more
doaj   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Governance and sustainability in Glasgow: connecting symbolic capital and housing consumption to regeneration [PDF]

, 2008
To transcend a legacy of slum-living, paternalistic provision and urban decline, Glasgow City Council has endeavoured to transform the city's fortunes by a plethora of mechanisms that have at their core the establishment of sustainable communities ...
McIntyre, Z., McKee, K., McKee, Kim, McIntyre, Zhan   +3 more
core   +1 more source

Bi-model optimization of a carbon tax for emission reduction

Cleaner and Responsible Consumption
The Paris Agreement mandated high-greenhouse-emitting countries implement carbon pricing to accelerate emission reduction to cope with environmental challenges posed by climate change.
Asif Hameed, Guozhu Mao, Adnan Ahmed Sheikh, Fadoua Kouki   +3 more
doaj   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Exploring the growth and influencing factors of carbon emissions in rural households of underdeveloped regions in China

Cleaner and Responsible Consumption
Carbon emissions from rural households in underdeveloped regions remain poorly understood compared to those in urban and developed areas. This study analyzes household carbon emissions in Xunyang County, China, demonstrating that food-related emissions ...
Jianbin Xu, Jie Song, Baochao Li
doaj   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source