Results 211 to 220 of about 186,000 (245)

Cloned restriction-modification systems — a review

Gene, 1988
The genes for numerous restriction endonucleases and modification methylases have been cloned into Escherichia coli. A summary is given for the clones isolated so far (115 entries) and of the procedures used to obtain them.
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Organization and sequence of the SalI restriction-modification system

Gene, 1994
The organization and nucleotide (nt) sequences were determined for the genes encoding the SalI restriction and modification (R-M) system (recognition sequence 5'-GTCGAC-3') from Streptomyces albus G. The system comprises two genes, salIR, coding for the restriction endonuclease (ENase, R.SalI; probably 315 amino acids (aa), a predicted M(r) of 35,305 ...
M R, Rodicio, T, Quinton-Jager, L S, Moran, B E, Slatko, G G, Wilson   +4 more
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Cloning of the HhaI and HinPI restriction-modification systems

Gene, 1988
The genes for the HhaI (Roberts et al., 1976) and HinPI (Roberts, 1987) restriction-modification (R-M) systems have been cloned in pBR322. The HhaI system was isolated on a 9-kb PstI fragment, and the HinPI system was isolated on two PstI fragments of 1.5 and 4.6 kb in length.
J M, Barsomian, C O, Card, G G, Wilson
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Restriction-modification systems in Streptomyces antibioticus

Canadian Journal of Microbiology, 1985
Several restriction systems were detected in different strains of Streptomyces antibioticus by using actinophages as biological indicators. Adsorption of phages to the bacteria, together with the study of the efficiency of plating gave an initial indication of restriction in three strains.
J, Sànchez, C, Barbès, A, Hernandez, C R, de los Reyes Gavilàn, C, Hardisson   +4 more
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Type II restriction—modification systems

Trends in Genetics, 1988
Abstract The genes for increasing numbers of type II restriction and modification enzymes are being cloned and characterized. The gene arrangements and enzyme sequences are quite diverse in spite of the biochemical similarities of the enzymes.
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Restriction-modification systems determined by Pseudomonas plasmids

Plasmid, 1982
Abstract Four additional Pseudomonas R plasmids determining the Pae R7 restriction-modification system have been detected. All are transfer deficient and appear to belong to the same incompatibility group. The Pseudomonas fertility plasmid FP110 determines a different restriction-modification system and also inhibits the propagation of phage B39
G A, Jacoby, L, Sutton
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Cloning the BstVI restriction-modification system in Escherichia coli

Gene, 1991
A standard DNA modification methyltransferase (MTase) selection protocol was followed to clone the BstVI restriction and modification system from Bacillus stearothermophilus in Escherichia coli. Both genes were contained in a 4.4-kb EcoRI fragment from B. stearothermophilus V chromosomal DNA. The heterologous expression of these genes did not depend on
C Vásquez
exaly   +3 more sources

Role of restriction-modification systems in prokaryotic evolution and ecology

Biochemistry (Moscow), 2015
Restriction-modification (R-M) systems are able to methylate or cleave DNA depending on methylation status of their recognition site. It allows them to protect bacterial cells from invasion by foreign DNA. Comparative analysis of a large number of available bacterial genomes and methylomes clearly demonstrates that the role of R-M systems in bacteria ...
A S, Ershova, I S, Rusinov, S A, Spirin, A S, Karyagina, A V, Alexeevski   +4 more
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Characterization of AloI, a restriction-modification system of a new type

Journal of Molecular Biology, 2001
We report the properties of the new AloI restriction and modification enzyme from Acinetobacter lwoffi Ks 4-8 that recognizes the DNA target 5' GGA(N)6GTTC3' (complementary strand 5' GAAC(N)6TCC3'), and the nucleotide sequence of the gene encoding this enzyme.
M Petrušytė, Z Maneliene, Albertas Timinskas   +2 more
exaly   +3 more sources

Cloning the KpnI restriction-modification system in Escherichia coli

Gene, 1991
The genes encoding the KpnI restriction and modification (R-M) system from Klebsiella pneumoniae, recognizing the sequence, 5'-GGTAC decreases C-3', were cloned and expressed in Escherichia coli. Although the restriction endonuclease (ENase)- and methyltransferase (MTase)-encoding genes were closely linked, initial attempts to clone both genes as a ...
A W, Hammond, G F, Gerard, D K, Chatterjee   +2 more
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