Various plasmid strategies limit the effect of bacterial restriction-modification systems against conjugation. [PDF]
Nucleic Acids ResIn bacteria, genes conferring antibiotic resistance are mostly carried on conjugative plasmids, mobile genetic elements which spread horizontally between bacterial hosts. Bacteria carry defence systems which defend them against genetic parasites, but how
Dimitriu T, Szczelkun MD, Westra ER.
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Nutrient Loading and Viral Memory Drive Accumulation of Restriction Modification Systems in Bloom-Forming Cyanobacteria. [PDF]
mBio, 2021 Harmful algal blooms (HABs), caused by cyanobacteria like Microcystis aeruginosa, are a global threat to water quality and use across the planet. Researchers have agreed that nutrient loading is a major contributor to HAB persistence.
Papoulis SE +3 more
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Annual Review of Microbiology, 2020 Human-adapted bacterial pathogens use a mechanism called phase variation to randomly switch the expression of individual genes to generate a phenotypically diverse population to adapt to challenges within and between human hosts.
Atack, JM +3 more
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Interplay Between Bacteriophages and Restriction-Modification Systems in Enterococci
Nova Biotechnologica et Chimica, 2014 The complete genomes of Enterococcus faecalis bacteriophages were analyzed for tetranucleotide words avoidance. Very similar tetranucleotide composition was found in all tested genomes with strong underrepresentation of palindromic GATC and GGCC words ...
Pristas Peter +2 more
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The Hyperthermophilic Restriction-Modification Systems of Thermococcus kodakarensis Protect Genome Integrity [PDF]
Frontiers in Microbiology, 2021 Thermococcus kodakarensis (T. kodakarensis), a hyperthermophilic, genetically accessible model archaeon, encodes two putative restriction modification (R-M) defense systems, TkoI and TkoII.
Kelly M. Zatopek +6 more
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Genetic variation regulates the activation and specificity of Restriction-Modification systems in Neisseria gonorrhoeae. [PDF]
Sci Rep, 2019 Restriction-Modification systems (RMS) are one of the main mechanisms of defence against foreign DNA invasion and can have an important role in the regulation of gene expression.
Sánchez-Busó L +4 more
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Rational development of transformation in Clostridium thermocellum ATCC 27405 via complete methylome analysis and evasion of native restriction-modification systems. [PDF]
J Ind Microbiol Biotechnol, 2019 A major barrier to both metabolic engineering and fundamental biological studies is the lack of genetic tools in most microorganisms. One example is Clostridium thermocellum ATCC 27405T, where genetic tools are not available to help validate decades of ...
Riley LA +4 more
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Phase-variable methylation and epigenetic regulation by type I restriction–modification systems
FEMS Microbiology Reviews, 2017 Epigenetic modifications in bacteria, such as DNA methylation, have been shown to affect gene regulation, thereby generating cells that are isogenic but with distinctly different phenotypes.
Megan De Ste Croix +2 more
exaly +2 more sources
Structural and functional diversity among Type III restriction-modification systems that confer host DNA protection via methylation of the N4 atom of cytosine. [PDF]
PLoS ONE, 2021 We report a new subgroup of Type III Restriction-Modification systems that use m4C methylation for host protection. Recognition specificities for six such systems, each recognizing a novel motif, have been determined using single molecule real-time DNA ...
Iain A Murray +9 more
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DNA Methylation by Restriction Modification Systems Affects the Global Transcriptome Profile in Borrelia burgdorferi. [PDF]
J Bacteriol, 2018 Lyme disease is the most prevalent vector-borne disease in North America and is classified by the Centers for Disease Control and Prevention (CDC) as an emerging infectious disease with an expanding geographical area of occurrence.
Casselli T +6 more
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