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Restriction Enzymes from Thermophiles
2013Restriction endonucleases (REases) are enzymes that recognize and cleave DNA in a sequence specific manner. The recognition site consists of a sequence of nucleotides in the DNA duplex, typically four to eight base pairs long. Most of the commercially produced REases are isolated from the mesophilic bacteria.
Prince Sharma +2 more
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Restriction Enzyme Analysis of PCR Products
2009Progress in single nucleotide polymorphism (SNP) detection technologies has provided information for SNP-based studies, such as identification of candidate genes for the complex genetic diseases, pharmacogenetic analysis, drug development, population genetics, evolutionary studies, and forensic investigations. SNP detection is performed by many methods,
Hideki Asamura +3 more
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Differential reactivities at restriction enzyme sites
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1981A method has been developed to measure the rates of digestion by restriction enzymes at individual sites. This involves a simple arithmetical treatment of the integrated areas from a densitometer scan of an ethidium bromide stained gel. We have used this method to study the digestion by HpaI, HincII and SalI of pBR322 and phi X174 DNA, and the effect ...
John R. Moffatt, Alan D. B. Malcolm
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Protein Engineering of Restriction Enzymes
2004Restriction endonucleases are among the most accurate enzymes known (Pingoud and Jeltsch 2001). Consequently, changing their very high sequence specificity is one of the scientific goals in studying these enzymes (Jeltsch et al. 1996). This review focuses on protein engineering regarding Type II restriction enzymes.
Jürgen Alves, P. Vennekohl
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DNAase I footprinting of restriction enzymes
Biochemical and Biophysical Research Communications, 1988DNAase I footprinting of restriction enzymes has been achieved by using calcium containing digestion buffers so that the enzymes bind to but do not cleave DNA. EcoR1 produces a footprint 17 bases long, overestimating the region of contact with DNA by about 7-8 base pairs.
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Modifying Specificities of Restriction Enzymes
1991For the last five years, our laboratory has been studying various aspects of controlled cleavage of DNA, both for (1) the 50 bp-10 kb range of single-stranded (ss) and double-stranded (ds) DNA fragments, plasmids or phages, and (2) for very large genomes of bacteria, yeast and higher eukaryotes.
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Hopping, jumping and looping by restriction enzymes
Biochemical Society Transactions, 2001Type II restriction endonucleases recognize specific DNA sequences and cleave both strands of the DNA at fixed locations at or near their recognition sites. Many of these enzymes are dimeric proteins that recognize, in symmetrical fashion, palindromic DNA sequences.
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