Results 1 to 10 of about 3,087,992 (329)
Biomolecular computers with multiple restriction enzymes [PDF]
Genetics and Molecular Biology, 2017 The development of conventional, silicon-based computers has several limitations, including some related to the Heisenberg uncertainty principle and the von Neumann “bottleneck”.
Sebastian Sakowski +5 more
doaj +7 more sources
Type I restriction enzymes and their relatives [PDF]
Nucleic Acids Research, 2013 Type I restriction enzymes (REases) are large pentameric proteins with separate restriction (R), methylation (M) and DNA sequence-recognition (S) subunits. They were the first REases to be discovered and purified, but unlike the enormously useful Type II
W. Loenen +3 more
semanticscholar +6 more sources
Restriction enzyme body doubles and PCR cloning: on the general use of type IIs restriction enzymes for cloning. [PDF]
PLoS ONE, 2014 The procedure described here allows the cloning of PCR fragments containing a recognition site of the restriction endonuclease (Type IIP) used for cloning in the sequence of the insert.
Eszter Tóth +8 more
doaj +3 more sources
Cold Spring Harbor Protocols, 2021 Restriction enzymes provided the foundation on which molecular cloning was built, and they remain as essential tools in current recombinant DNA technology. The three classes of restriction enzymes and their features are introduced here.
Michael R Green, J. Sambrook
semanticscholar +3 more sources
REBASE: restriction enzymes and methyltransferases [PDF]
Nucleic Acids Research, 2003 REBASE contains comprehensive information about restriction enzymes, DNA methyltransferases and related proteins such as nicking enzymes, specificity subunits and control proteins. It contains published and unpublished references, recognition and cleavage sites, isoschizomers, commercial availability, crystal and sequence data. Homing endonucleases are
R. Roberts +3 more
semanticscholar +4 more sources
REBASE: a database for DNA restriction and modification: enzymes, genes and genomes
Nucleic Acids Res., 2022 REBASE is a comprehensive and extensively curated database of information about the components of restriction-modification (RM) systems. It is fully referenced and provides information about the recognition and cleavage sites for both restriction enzymes
R. Roberts +3 more
semanticscholar +1 more source
Historical Aspects of Restriction Endonucleases as Intelligent Scissors for Genetic Engineering
Fermentation, 2023 Restriction endonucleases are a component of restriction–modification systems, where the main biological function is to protect bacterial cells from incoming foreign DNA molecules.
Irina V. Alekseeva, Nikita A. Kuznetsov
doaj +1 more source
REBASE--restriction enzymes and methylases [PDF]
Nucleic Acids Research, 1993 REBASE is a comprehensive database of information about restriction enzymes and related proteins. It contains published and unpublished references, recognition and cleavage sites, isoschizomers, commercial availability, methylation sensitivity, crystal and sequence data.
Richard J. Roberts, Dana Macelis
openaire +16 more sources
A CRISPR-based approach using dead Cas9-sgRNA to detect SARS-CoV-2
Frontiers in Molecular Biosciences, 2023 Rapid, highly specific, and robust diagnostic kits to detect viruses and pathogens are needed to control disease spread and transmission globally. Of the many different methods proposed to diagnose COVID-19 infection, CRISPR-based detection of nucleic ...
Mustapha Aouida +8 more
doaj +1 more source
Putative RFLP Analysis Between HSVd-sycv and Closely Related Variants [PDF]
Journal of Cell and Molecular Research, 2016 Yellow corky vein is a prevalent disease among navel oranges in the Fars province of Iran. Previously we showed that a variant of Hop stunt viroid (HSVd-sycv) was associated with the disease.
seyed ali akbar Bagherian
doaj +1 more source