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Biomolecular computers with multiple restriction enzymes [PDF]
The development of conventional, silicon-based computers has several limitations, including some related to the Heisenberg uncertainty principle and the von Neumann “bottleneck”.
Sebastian Sakowski +5 more
doaj +3 more sources
Restriction enzyme body doubles and PCR cloning: on the general use of type IIs restriction enzymes for cloning. [PDF]
The procedure described here allows the cloning of PCR fragments containing a recognition site of the restriction endonuclease (Type IIP) used for cloning in the sequence of the insert.
Eszter Tóth +8 more
doaj +3 more sources
Nucleoside triphosphate-dependent restriction enzymes [PDF]
The known nucleoside triphosphate-dependent restriction enzymes are hetero-oligomeric proteins that behave as molecular machines in response to their target sequences. They translocate DNA in a process dependent on the hydrolysis of a nucleoside triphosphate.
Dryden, DTF, Murray, Noreen E, Rao, DN
semanticscholar +5 more sources
REBASE--restriction enzymes and methylases [PDF]
REBASE is a comprehensive database of information about restriction enzymes and related proteins. It contains published and unpublished references, recognition and cleavage sites, isoschizomers, commercial availability, methylation sensitivity, crystal and sequence data.
R J, Roberts, D, Macelis
openaire +17 more sources
Restriction enzymes provided the foundation on which molecular cloning was built, and they remain as essential tools in current recombinant DNA technology. The three classes of restriction enzymes and their features are introduced here.
Michael R, Green, Joseph, Sambrook
openaire +3 more sources
Historical Aspects of Restriction Endonucleases as Intelligent Scissors for Genetic Engineering
Restriction endonucleases are a component of restriction–modification systems, where the main biological function is to protect bacterial cells from incoming foreign DNA molecules.
Irina V. Alekseeva, Nikita A. Kuznetsov
doaj +1 more source
A CRISPR-based approach using dead Cas9-sgRNA to detect SARS-CoV-2
Rapid, highly specific, and robust diagnostic kits to detect viruses and pathogens are needed to control disease spread and transmission globally. Of the many different methods proposed to diagnose COVID-19 infection, CRISPR-based detection of nucleic ...
Mustapha Aouida +8 more
doaj +1 more source
REHUNT: a reliable and open source package for restriction enzyme hunting
Background Restriction enzymes are used frequently in biotechnology. However, manual mining of restriction enzymes is challenging. Furthermore, integrating available restriction enzymes into different bioinformatics systems is necessary for many ...
Yu-Huei Cheng +2 more
doaj +1 more source
A physical map of human Alu repeats cleavage by restriction endonucleases
Background Alu repetitive elements are the abundant sequences in human genome. Diversity of DNA sequences of these elements makes difficult the construction of theoretical patterns of Alu repeats cleavage by restriction endonucleases.
Chernukhin Valery A +3 more
doaj +1 more source
Putative RFLP Analysis Between HSVd-sycv and Closely Related Variants [PDF]
Yellow corky vein is a prevalent disease among navel oranges in the Fars province of Iran. Previously we showed that a variant of Hop stunt viroid (HSVd-sycv) was associated with the disease.
seyed ali akbar Bagherian
doaj +1 more source

