Results 161 to 170 of about 486,664 (294)

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Diffdigester.uni-jena.de: a tool for optimized selection of restriction enzymes for plasmid identification in cloning procedures. [PDF]

Nucleic Acids Res
Gühmann M, Reuter S, Mrowka R.
europepmc   +1 more source

Further Probing of Cu²⁺-Dependent PNAzymes Acting as Artificial RNA Restriction Enzymes. [PDF]

Molecules, 2019
Luige O, Murtola M, Ghidini A, Strömberg R.   +3 more
europepmc   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

Using nucleolytic toxins as restriction enzymes enables new RNA applications. [PDF]

Nucleic Acids Res
Rothweiler U, Gundesø SE, Mikalsen EW, Svenning S, Singh M, Combes F, Pettersson FJ, Mangold A, Piotrowski Y, Schwab F, Lanes O, Striberny BK.   +11 more
europepmc   +1 more source

Restriction Enzymes as a Target for DNA-Based Sensing and Structural Rearrangement. [PDF]

ACS Omega, 2018
Buckhout-White S, Person C, Medintz IL, Goldman ER.   +3 more
europepmc   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

An enterococcal phage protein inhibits type IV restriction enzymes involved in antiphage defense. [PDF]

Nat Commun
Bullen NP, Johnson CN, Andersen SE, Arya G, Marotta SR, Lee YJ, Weigele PR, Whitney JC, Duerkop BA.   +8 more
europepmc   +1 more source

Determining if DNA Stained with a Cyanine Dye Can Be Digested with Restriction Enzymes. [PDF]

J Vis Exp, 2018
Maschmann A, Masters C, Davison M, Lallman J, Thompson D, Kounovsky-Shafer KL.   +5 more
europepmc   +1 more source

PARP inhibitors induce a senescence phenotype in non‐small cell lung carcinoma cell lines

FEBS Open Bio, EarlyView.
Talazoparib is the most potent inducer of senescence among different PARP1 inhibitors in human NSCLC cells. In the absence of PARP, no senescence phenotype was observed, demonstrating that PARP1 is necessary for the induction of senescence by this inhibitor.
Camille Huart, Manon Van den Abbeel, Christoph Schifflers, Karim Bouhjar, Andrea Scarmelotto, Alexis Khelfi, Anne‐Catherine Wera, Carine Michiels   +7 more
wiley   +1 more source