Results 21 to 30 of about 2,915,798 (345)

Fused eco29kIR- and M genes coding for a fully functional hybrid polypeptide as a model of molecular evolution of restriction-modification systems

BMC Evolutionary Biology, 2011
Background The discovery of restriction endonucleases and modification DNA methyltransferases, key instruments of genetic engineering, opened a new era of molecular biology through development of the recombinant DNA technology.
Solonin Alexander S, Mokrishcheva Marina L, Nikitin Dmitri V   +2 more
doaj   +1 more source

Isolation, Purification and Crystallization of Restriction Enzyme Bsa I and Its Preparation of Seleno-derived Derivatives

Shipin gongye ke-ji, 2023
Objective: To screen and optimize the method for preparing three-dimensional structural samples of restriction endonuclease Bsa I. Methods: In this study, the Escherichia coli expression system was employed to express the Bsa I protein and its ...
Hai ZHU, Mengze ZHENG, Weiwei JIA, Qian ZHOU, Shuai TAN, Ziang LIU, Chuanzhi ZHANG, Weiling WANG, Tingting LI   +8 more
doaj   +1 more source

A NOVEL APPROACH TO GENOTYPING OF HOSPITAL ISOLATES OF CLOSTRIDIUM DIFFICILE

Журнал микробиологии, эпидемиологии и иммунобиологии, 2016
Aim. Development of a novel approach in genotyping of Clostridium difficile and its testing on the example of 140 hospital isolates. Materials and methods.
V. P. Terletsky, V. I. Tyschenko, O. B. Novikova, I. I. Novikova, E. D. Dzhavadov   +4 more
doaj   +1 more source

Morphology, morphometric and molecular study of geographical isolates of an entomopathogenic nematode, Steinernema feltiae (Rhabditida: Steinernematidae) from north-west of Iran [PDF]

نامه انجمن حشره‌شناسی ایران, 2017
The study was intended to investigate the morphological, morphometric and molecular characterization of eight geographical isolates of the entomopathogenic nematode Steinernema feltiae from northwest of Iran.
Naser Eivazian Kary, D. Mohammadi, Zhila Alizadeh   +2 more
doaj   +1 more source

Golden Gate Shuffling: A One-Pot DNA Shuffling Method Based on Type IIs Restriction Enzymes

PLoS ONE, 2009
We have developed a protocol to assemble in one step and one tube at least nine separate DNA fragments together into an acceptor vector, with 90% of recombinant clones obtained containing the desired construct.
Carola Engler, Ramona Gruetzner, R. Kandzia, S. Marillonnet   +3 more
semanticscholar   +1 more source

REtools: A laboratory program for restriction enzyme work: enzyme selection and reaction condition assistance

BMC Bioinformatics, 2006
Background Restriction enzymes are one of the everyday tools used in molecular biology. The continuously expanding panel of known restriction enzymes (several thousands) renders their optimal use virtually impossible without computerized assistance ...
Boulukos Kim E, Martin Patrick, Pognonec Philippe   +2 more
doaj   +1 more source

Restriction site heteroplasmy in the mitochondrial DNA of Brycon opalinus (Cuvier, 1819) (Characiformes, Characidae, Bryconiae)

Brazilian Journal of Medical and Biological Research, 2004
Homoplasmy is a feature usually found in the mtDNA of higher animal taxa. On the other hand, the presence of two classes of mtDNA in the same cell or organism is rare and may appear in length or site variation.
A.W.S. Hilsdorf, J.E. Krieger
doaj   +1 more source

Genotyping by Sequencing Advancements in Barley

Frontiers in Plant Science, 2022
Barley is considered an ideal crop to study cereal genetics due to its close relationship with wheat and diploid ancestral genome. It plays a crucial role in reducing risks to global food security posed by climate change. Genetic variations in the traits
Nirmal Raj Rajendran, Naeela Qureshi, Mohammad Pourkheirandish   +2 more
doaj   +1 more source

PCR-RFLP method in combination with on-chip electrophoresis as a tool for determining of variability between important species of Aspergillus [PDF]

Food and Feed Research
Aspergillus species are among the most significant producers of aflatoxins, which can contaminate a wide range of agricultural and food products at any stage of production.
Ćurčić Nataša Ž., Miljanić Jelena A., Bočarov-Stančić Aleksandra S., Vukelić Igor D., Bodroža-Solarov Marija I.   +4 more
doaj   +1 more source

REBASE—a database for DNA restriction and modification: enzymes, genes and genomes

Nucleic Acids Res., 2014
REBASE is a comprehensive and fully curated database of information about the components of restriction-modification (RM) systems. It contains fully referenced information about recognition and cleavage sites for both restriction enzymes and ...
R. Roberts, Tamas Vincze, J. Posfai, D. Macelis   +3 more
semanticscholar   +1 more source