Results 21 to 30 of about 3,087,992 (329)
Archaeal diversity in deep-sea sediments estimated by means of different Terminal-Restriction Fragment Length Polymorphisms (T-RFLP) protocols [PDF]
Despite the increasing recognition of the quantitative importance of Archaea in all marine systems, the protocols for a rapid estimate of Archaeal diversity patterns in deep-sea sediments have been only poorly tested yet.
Danovaro, Roberto +3 more
core +1 more source
The Architecture of Restriction Enzymes [PDF]
In this issue of Structure, Lyumkis and colleagues describe a high resolution structure of a polymerized form of the SgrAI restriction enzyme, which shows that it forms a helical assembly with four enzyme molecules per turn of the helix. The DNA is arranged on the periphery of the protein helix pointing away from the helical axis.
openaire +3 more sources
A NOVEL APPROACH TO GENOTYPING OF HOSPITAL ISOLATES OF CLOSTRIDIUM DIFFICILE
Aim. Development of a novel approach in genotyping of Clostridium difficile and its testing on the example of 140 hospital isolates. Materials and methods.
V. P. Terletsky +4 more
doaj +1 more source
pecific data base was used for restriction enzymes (rebase) and related proteins, to design executive program in quick basic language to determine the restriction sites and drawing restriction map for the complete sequence of lipase gene from Bacillus ...
Hameed M. Jasim
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Morphology, morphometric and molecular study of geographical isolates of an entomopathogenic nematode, Steinernema feltiae (Rhabditida: Steinernematidae) from north-west of Iran [PDF]
The study was intended to investigate the morphological, morphometric and molecular characterization of eight geographical isolates of the entomopathogenic nematode Steinernema feltiae from northwest of Iran.
Naser Eivazian Kary +2 more
doaj +1 more source
Pathological phenotypes and in vivo DNA cleavage by unrestrained activity of a phosphorothioate-based restriction system in Salmonella [PDF]
Prokaryotes protect their genomes from foreign DNA with a diversity of defence mechanisms, including a widespread restriction–modification (R–M) system involving phosphorothioate (PT) modification of the DNA backbone. Unlike classical R–M systems, highly
Cao, Bo +8 more
core +1 more source
Background The discovery of restriction endonucleases and modification DNA methyltransferases, key instruments of genetic engineering, opened a new era of molecular biology through development of the recombinant DNA technology.
Solonin Alexander S +2 more
doaj +1 more source
Background Restriction enzymes are one of the everyday tools used in molecular biology. The continuously expanding panel of known restriction enzymes (several thousands) renders their optimal use virtually impossible without computerized assistance ...
Boulukos Kim E +2 more
doaj +1 more source
Characterization of Ascaris from Ecuador and Zanzibar [PDF]
To shed light on the epidemiology of ascariasis in Ecuador and Zanzibar, 177 adult worms retrieved by chemo-expulsion from either people or pigs were collected, measured and subjected to polymerase chain reaction-restriction fragment length polymorphism (
A.M. Sparks +9 more
core +5 more sources
A Sliding Restriction Enzyme Pauses [PDF]
In this issue of Structure, Aggarwal and colleagues (Townson et al., 2007) present the crystal structure of the restriction endonuclease BstYI in complex with a near-cognate substrate. This structure most likely reflects the conformation BstYI adopts as it scans DNA and pauses upon encountering a site similar to its recognition sequence.
Wolfgang Wende, Alfred Pingoud
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