Results 71 to 80 of about 3,087,992 (329)

Comparative genomics evidence that only protein toxins are tagging bad bugs

Frontiers in Cellular and Infection Microbiology, 2011
The term toxin was introduced by Roux and Yersin and describes macromolecular substances that, when produced during infection or when introduced parenterally or orally, cause an impairment of physiological functions that lead to disease or to the death ...
Kalliopi eGeorgiades, Didier eRaoult
doaj   +1 more source

Application of 16S rRNA virtual RFLP for the discrimination of some closely taxonomic-related lactobacilli species

Journal of Genetic Engineering and Biotechnology, 2022
Background Several species in Lactobacillaceae family were recognized as potential probiotic bacteria. In this group of lactic acid bacteria, species are taxonomically closed and usually share similar 16S rRNA gene, thus, instead of so their ...
Nora Laref, Khadidja Belkheir
doaj   +1 more source

A key to selected rockfishes (Sebastes spp.) based on mitochondrial DNA restriction fragment analysis [PDF]

, 2006
Larval and juvenile rockfishes (Sebastes spp.) are difficult to identify using morphological characters. We developed a key based on sizes of restriction endonuclease fragments of the NADH dehydrogenase-3 and -4 (ND3/ND4) and 12S and 16S ribosomal RNA ...
Asahida, Takashi, Gharrett, Anthony J., Goto, Akira, Gray, Andrew K., Li, Zhuozhuo, Love, Milton S.   +5 more
core  

Molecular characterization of Salmonella Enteritidis : comparison of an optimized multi-locus variable-number of tandem repeat analysis (MLVA) and pulsed-field gel electrophoresis [PDF]

, 2012
Salmonella Enteritidis (SE) is a genetically homogenous serovar, which makes optimal subtype discrimination crucial for epidemiological research. This study describes the development and evaluation of an optimized multiple-locus variable number tandem ...
Bertrand, Sophie, De Reu, Koen, Dewaele, Isabelle, Ducatelle, Richard, Herman, Lieve, Heyndrickx, Marc, Imberechts, Hein, Rasschaert, Geertrui, Wattiau, Pierre, Wildemauwe, Christa   +9 more
core   +2 more sources

The epithelial barrier theory proposes a comprehensive explanation for the origins of allergic and other chronic noncommunicable diseases

FEBS Letters, EarlyView.
Exposure to common noxious agents (1), including allergens, pollutants, and micro‐nanoplastics, can cause epithelial barrier damage (2) in our body's protective linings. This may trigger an immune response to our microbiome (3). The epithelial barrier theory explains how this process can lead to chronic noncommunicable diseases (4) affecting organs ...
Can Zeyneloglu, Huseyn Babayev, Ismail Ogulur, Sena Ardicli, Yagiz Pat, Duygu Yazici, Bingjie Zhao, Lihong Chang, Xiaoqing Liu, Paolo D'Avino, Manru Li, Ceren Biçer, Fatma Hacer Kurtoğlu Babayev, Raja Dhir, Kari C. Nadeau, Marie‐Charlotte Brüggen, Mubeccel Akdis, Cezmi A. Akdis   +17 more
wiley   +1 more source

Extended Stability of Restriction Enzymes at Ambient Temperatures

BioTechniques, 2000
The stability of restriction enzymes as supplied by manufacturers without any modification has been examined. No reduction in activity was observed for three enzymes (HindIII, EcoRI and Tsp509I) held at ambient temperature or 4°C for the period of study (
Jason Clark, Jennifer C. Harrison, Robinson H. Mdegela, John B. March   +3 more
doaj   +1 more source

On the DNA cleavage mechanism of Type I restriction enzymes [PDF]

, 2017
Although the DNA cleavage mechanism of Type I restriction-modification enzymes has been extensively studied, the mode of cleavage remains elusive. In this work, DNA ends produced by EcoKI, EcoAI and EcoR124I, members of the Type IA, IB and IC families ...
Bickle, Thomas A., Hamburger, Fabienne, Janscak, Pavel, Jindrova, Eva, Schmid-Nuoffer, Stefanie   +4 more
core  

From omics to AI—mapping the pathogenic pathways in type 2 diabetes

FEBS Letters, EarlyView.
Integrating multi‐omics data with AI‐based modelling (unsupervised and supervised machine learning) identify optimal patient clusters, informing AI‐driven accurate risk stratification. Digital twins simulate individual trajectories in real time, guiding precision medicine by matching patients to targeted therapies.
Siobhán O'Sullivan, Lu Qi, Pierre Zalloua   +2 more
wiley   +1 more source

Sequence-specific cleavage of RNA by Type II restriction enzymes

Nucleic Acids Research, 2010
The ability of 223 Type II restriction endonucleases to hydrolyze RNA–DNA heteroduplex oligonucleotide substrates was assessed. Despite the significant topological and sequence asymmetry introduced when one strand of a DNA duplex is substituted by RNA we
I. Murray, S. Stickel, R. Roberts
semanticscholar   +1 more source

Thermostable neutral metalloprotease from Geobacillus sp. EA1 does not share thermolysin's preference for substrates with leucine at the P1′ position

FEBS Letters, EarlyView.
Knowing how proteases recognise preferred substrates facilitates matching proteases to applications. The S1′ pocket of protease EA1 directs cleavage to the N‐terminal side of hydrophobic residues, particularly leucine. The S1′ pocket of thermolysin differs from EA's at only one position (leucine in place of phenylalanine), which decreases cleavage ...
Grant R. Broomfield, Torsten Kleffmann, David J. Saul, Sigurd M. Wilbanks   +3 more
wiley   +1 more source