Results 71 to 80 of about 2,915,798 (345)

Genomic-based restriction enzyme selection for specific detection of Piscirickettsia salmonis by 16S rDNA PCR-RFLP

Frontiers in Microbiology, 2016
The gram negative facultative bacterium P. salmonis is the etiological agent of Salmonid Rickettsial Septicaemia (SRS), a severe disease that causes important economic losses in the global salmon farmer industry.
Dinka eMandakovic, Dinka eMandakovic, Benjamín eGlasner, Jonathan eMaldonado, Jonathan eMaldonado, Pamela eAravena, Pamela eAravena, Mauricio eGonzalez, Mauricio eGonzalez, Mauricio eGonzalez, Verónica eCambiazo, Verónica eCambiazo, Verónica eCambiazo, Rodrigo ePulgar, Rodrigo ePulgar, Rodrigo ePulgar   +15 more
doaj   +1 more source

Structural and evolutionary classification of Type II restriction enzymes based on theoretical and experimental analyses

Nucleic Acids Research, 2008
For a very long time, Type II restriction enzymes (REases) have been a paradigm of ORFans: proteins with no detectable similarity to each other and to any other protein in the database, despite common cellular and biochemical function.
Jerzy Orlowski, J. Bujnicki
semanticscholar   +1 more source

Peptide‐based ligand antagonists block a Vibrio cholerae adhesin

FEBS Letters, EarlyView.
The structure of a peptide‐binding domain of the Vibrio cholerae adhesin FrhA was solved by X‐ray crystallography, revealing how the inhibitory peptide AGYTD binds tightly at its Ca2+‐coordinated pocket. Structure‐guided design incorporating D‐amino acids enhanced binding affinity, providing a foundation for developing anti‐adhesion therapeutics ...
Mingyu Wang, Grace Du, Charity Yongo‐Luwawa, Angelina Lu, Brett Kinrade, Kim Munro, Karl E. Klose, William D. Lubell, Peter Davies, Shuaiqi Guo   +9 more
wiley   +1 more source

Diversidade genética de Begomovirus que infectam plantas invasoras na região nordeste Genetic diversity of Begomovirus infecting weeds in northeastern Brazil

Planta Daninha, 2006
Os Begomovirus fazem parte de uma família numerosa de fitovírus denominada Geminiviridae. Eles infectam ampla gama de hospedeiras, incluindo muitas espécies cultivadas, como tomate (Lycopersicon esculentum), feijão (Phaseolus vulgaris), pimentão ...
I.P. Asssunção, A.F. Listik, M.C.S. Barros, E.P.R. Amorin, S.J.C. Silva, Izael O. Silva, C.E. Ramalho-Neto, G.S.A. Lima   +7 more
doaj   +1 more source

Evaluating restriction enzyme selection for reduced representation sequencing in conservation genomics [PDF]

, 2023
A Leiro Lopez, Carlos Carreras, Marta Pascual, Cinta Pegueroles   +3 more
openalex   +1 more source

Interplay between circadian and other transcription factors—Implications for cycling transcriptome reprogramming

FEBS Letters, EarlyView.
This perspective highlights emerging insights into how the circadian transcription factor CLOCK:BMAL1 regulates chromatin architecture, cooperates with other transcription factors, and coordinates enhancer dynamics. We propose an updated framework for how circadian transcription factors operate within dynamic and multifactorial chromatin landscapes ...
Xinyu Y. Nie, Jerome S. Menet
wiley   +1 more source

Universal Linker and Ligation Procedures for Construction of Genomic DNA Libraries Enriched for Microsatellites

BioTechniques, 1999
Microsatellite loci are highly informative genetic markers useful for population genetic studies, linkage mapping and parentage determination. Methods to identify novel microsatellite loci commonly use subtractive hybridization to enrich smallinsert ...
Matthew B. Hamilton, Elaine L. Pincus, Anthony Di Fiore, Robert C. Fleischer   +3 more
doaj   +1 more source

Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer

FEBS Letters, EarlyView.
Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani, Noemi E. Nwosu, Viktoria Gocke, Müge Kasanmascheff, Hannes Mutschler   +4 more
wiley   +1 more source

Novel protein fold discovered in the PabI family of restriction enzymes

Nucleic Acids Research, 2007
Although structures of many DNA-binding proteins have been solved, they fall into a limited number of folds. Here, we describe an approach that led to the finding of a novel DNA-binding fold. Based on the behavior of Type II restriction–modification gene
K. Miyazono, Miki Watanabe, J. Kosiński, Ken Ishikawa, M. Kamo, T. Sawasaki, K. Nagata, J. Bujnicki, Y. Endo, M. Tanokura, I. Kobayashi   +10 more
semanticscholar   +1 more source

Disordered but rhythmic—the role of intrinsic protein disorder in eukaryotic circadian timing

FEBS Letters, EarlyView.
Unstructured domains known as intrinsically disordered regions (IDRs) are present in nearly every part of the eukaryotic core circadian oscillator. IDRs enable many diverse inter‐ and intramolecular interactions that support clock function. IDR conformations are highly tunable by post‐translational modifications and environmental conditions, which ...
Emery T. Usher, Jacqueline F. Pelham
wiley   +1 more source