Results 81 to 90 of about 2,915,798 (345)
An Efficient PCR-RFLP Method for the Rapid Identification of Korean Pyropia Species
Molecules, 2017 The present study utilizes polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis using partial plastid rbcL and mitochondrial trnC–trnP gene sequences to distinguish the six representative Pyropia species produced via ...
Yonguk Kim, Sung-Je Choi, Chulyung Choi
doaj +1 more source
ATP-dependent restriction enzyme SauUSI from Staphylococcus aureus is also a bona fide single strand DNA endonuclease [PDF]
, 2022 Vinayak Sadasivam Tumuluri +1 more
openalex +1 more source
Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation
FEBS Letters, EarlyView.Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe +3 more
wiley +1 more source
Journal of Genetic Engineering and Biotechnology, 2018 Klebsiella pneumoniae is the second leading causative agent of UTI. In this study, a rapid combined polymerase chain reaction and restriction fragment length polymorphism analysis was developed to identify K.
Golam Mahmudunnabi +5 more
doaj +1 more source
The Architecture of Restriction Enzymes [PDF]
Structure, 2013 In this issue of Structure, Lyumkis and colleagues describe a high resolution structure of a polymerized form of the SgrAI restriction enzyme, which shows that it forms a helical assembly with four enzyme molecules per turn of the helix. The DNA is arranged on the periphery of the protein helix pointing away from the helical axis.
openaire +2 more sources
Real-time observation of DNA looping dynamics of Type IIE restriction enzymes NaeI and NarI
Nucleic Acids Research, 2006 Many restriction enzymes require binding of two copies of a recognition sequence for DNA cleavage, thereby introducing a loop in the DNA. We investigated looping dynamics of Type IIE restriction enzymes NaeI and NarI by tracking the Brownian motion of ...
Bram van den Broek +4 more
semanticscholar +1 more source
The role of histone modifications in transcription regulation upon DNA damage
FEBS Letters, EarlyView.This review discusses the critical role of histone modifications in regulating gene expression during the DNA damage response (DDR). By modulating chromatin structure and recruiting repair factors, these post‐translational modifications fine‐tune transcriptional programmes to maintain genomic stability.
Angelina Job Kolady, Siyao Wang
wiley +1 more source
PCR-RFLP of mitochondrial DNA of swamp buffaloes in breeding station
Italian Journal of Animal Science, 2010 Methods have been devised for detecting polymorphisms in the D-loop regions using the PCR followed by restriction enzyme digestion to reveal restriction fragment length polymorphism.
M. Kamonpatana +6 more
doaj +1 more source
Nucleic Acids Research, 2006 Hypermethylation of CpG island (CGI) sequences is a nearly universal somatic genome alteration in cancer. Rapid and sensitive detection of DNA hypermethylation would aid in cancer diagnosis and risk stratification.
S. Yegnasubramanian +4 more
semanticscholar +1 more source
FEBS Letters, EarlyView.Multidrug transporters BpeB and BpeF from the Gram‐negative pathogen Burkholderia pseudomallei have a hydrophilic patch in their substrate‐binding pocket. Drug susceptibility tests and growth curve analyses using an Escherichia coli recombinant expression system revealed that the hydrophilic patches of BpeB and BpeF are involved in the substrate ...
Ui Okada, Satoshi Murakami
wiley +1 more source