Results 151 to 160 of about 2,173,029 (360)

Characterising the role of GABA and its metabolism in the wheat pathogen Stagonospora nodorum [PDF]

, 2015
A reverse genetics approach was used to investigate the role of γ-aminobutyric acid metabolism in the wheat pathogenic fungus Stagonospora nodorum. The creation of mutants lacking Sdh1, the gene encoding succinic semialdehyde dehydrogenase, resulted in ...
Mead, Oliver, Solomon, Peter S., Thynne, Eli, Winterberg, Britta   +3 more
core   +1 more source

Inhibiting stearoyl‐CoA desaturase suppresses bone metastatic prostate cancer by modulating cellular stress, mTOR signaling, and DNA damage response

FEBS Letters, EarlyView.
Bone metastasis in prostate cancer (PCa) patients is a clinical hurdle due to the poor understanding of the supportive bone microenvironment. Here, we identify stearoyl‐CoA desaturase (SCD) as a tumor‐promoting enzyme and potential therapeutic target in bone metastatic PCa.
Alexis Wilson, Mackenzie K Herroon, Shane Mecca, Laimar C Garmo, Jacob Lindquist, Shrila Rajendran, Steve M. Patrick, Izabela Podgorski   +7 more
wiley   +1 more source

Reverse Genetics with a Full-length Infectious cDNA Clone of Bovine Torovirus [PDF]

, 2020
Makoto Ujike, Etoh Yuka, Urushiyama Naoya, Fumihiro Taguchi, Luis Enjuanes, Wataru Kamitani   +5 more
openalex   +1 more source

A reverse genetics system for avian coronavirus infectious bronchitis virus based on targeted RNA recombination

Virology Journal, 2017
BackgroundAvian coronavirus infectious bronchitis virus (IBV) is a respiratory pathogen of chickens that causes severe economic losses in the poultry industry worldwide.
S. V. van Beurden, A. Berends, Annika Krämer-Kühl, Dieuwertje Spekreijse, G. Chénard, H. Philipp, E. Mundt, P. J. M. Rottier, M. H. Verheije   +8 more
semanticscholar   +1 more source

Characterizing the salivary RNA landscape to identify potential diagnostic, prognostic, and follow‐up biomarkers for breast cancer

Molecular Oncology, EarlyView.
This study explores salivary RNA for breast cancer (BC) diagnosis, prognosis, and follow‐up. High‐throughput RNA sequencing identified distinct salivary RNA signatures, including novel transcripts, that differentiate BC from healthy controls, characterize histological and molecular subtypes, and indicate lymph node involvement.
Nicholas Rajan, Irina Primac, Emre Etlioglu, Laurens Debruyne, Ann Janssen, Magy Sallam, Kevin Tabury, Roel Quintens, Wiebren Tjalma, Mohammed Abderrafi Benotmane   +9 more
wiley   +1 more source

SARS-CoV-2 Reverse Genetics Reveals a Variable Infection Gradient in the Respiratory Tract

Cell, 2020
Yixuan J. Hou, K. Okuda, C. Edwards, David R. Martinez, T. Asakura, K. Dinnon, T. Kato, Rhianna E. Lee, B. Yount, T. Mascenik, Gang Chen, K. Olivier, A. Ghio, Longping V. Tse, S. Leist, Lisa E. Gralinski, A. Schäfer, H. Dang, R. Gilmore, S. Nakano, Ling Sun, M. Fulcher, Alessandra Livraghi-Butrico, N. Nicely, M. Cameron, Cheryl C. Cameron, D. Kelvin, A. D. de Silva, D. Margolis, A. Markmann, L. Bartelt, R. Zumwalt, F. Martinez, S. Salvatore, A. Borczuk, P. R. Tata, Vishwaraj Sontake, A. Kimple, I. Jaspers, W. O’Neal, S. Randell, R. Boucher, R. Baric   +42 more
semanticscholar   +1 more source

Comparative aspects of early lineage specification events in mammalian embryos – insights from reverse genetics studies

Cell Cycle, 2018
Within the mammalian class, formation of the blastocyst is morphologically highly conserved among different species. The molecular and cellular events during preimplantation embryo development have been studied extensively in the mouse as model organism,
K. Simmet, V. Zakhartchenko, E. Wolf
semanticscholar   +1 more source

Adenosine‐to‐inosine editing of miR‐200b‐3p is associated with the progression of high‐grade serous ovarian cancer

Molecular Oncology, EarlyView.
A‐to‐I editing of miRNAs, particularly miR‐200b‐3p, contributes to HGSOC progression by enhancing cancer cell proliferation, migration and 3D growth. The edited form is linked to poorer patient survival and the identification of novel molecular targets.
Magdalena Niemira, Anna Skwarska, Karolina Chwialkowska, Agnieszka Ostrowska, Gabriela Sokolowska, Anna Zeller, Anna Erol, Andrzej Eljaszewicz, Bartosz Hanczaruk, Anna Michalska‐Falkowska, Agnieszka Tarasik, Joanna Reszec‐Gielazyn, Pawel Knapp, Marcin Moniuszko, Adam Kretowski   +14 more
wiley   +1 more source

Integrated genomic and proteomic profiling reveals insights into chemoradiation resistance in cervical cancer

Molecular Oncology, EarlyView.
A comprehensive genomic and proteomic analysis of cervical cancer revealed STK11 and STX3 as a potential biomarkers of chemoradiation resistance. Our study demonstrated EGFR as a therapeutic target, paving the way for precision strategies to overcome treatment failure and the DNA repair pathway as a critical mechanism of resistance.
Janani Sambath, Irene A. George, Srikanth S. Manda, Prasanth Ariyannur, Ekta R. Dhawale, Raja Sekhar Kommu, Rajan Datar, Darshana Patil, Vinita Trivedi, Manisha Singh, Kumar Prabhash, Sewanti Limaye, Richa Chauhan, Prashant Kumar   +13 more
wiley   +1 more source

Reverse Genetics for Fusogenic Bat-Borne Orthoreovirus Associated with Acute Respiratory Tract Infections in Humans: Role of Outer Capsid Protein σC in Viral Replication and Pathogenesis.

PLoS Pathogens, 2016
Nelson Bay orthoreoviruses (NBVs) are members of the fusogenic orthoreoviruses and possess 10-segmented double-stranded RNA genomes. NBV was first isolated from a fruit bat in Australia more than 40 years ago, but it was not associated with any disease ...
Takahiro Kawagishi, Yuta Kanai, Hideki Tani, Masayuki Shimojima, Masayuki Saijo, Yoshiharu Matsuura, Takeshi Kobayashi   +6 more
doaj   +1 more source