Results 231 to 240 of about 668,266 (290)

In vitro one-pot construction of influenza viral genomes for virus particle synthesis based on reverse genetics system. [PDF]

PLoS One
Tanaka R, Tamao K, Ono M, Yamayoshi S, Kawaoka Y, Su'etsugu M, Noji H, Tabata KV.   +7 more
europepmc   +1 more source

CRISPR/Cas9 screens identify key host factors that enhance rotavirus reverse genetics efficacy and vaccine production. [PDF]

NPJ Vaccines
Zhu Y, Sullender ME, Campbell DE, Wang L, Lee S, Kawagishi T, Hou G, Dizdarevic A, Jais PH, Baldridge MT, Ding S.   +10 more
europepmc   +1 more source

Development of a robust and efficient virus-induced gene silencing system for reverse genetics in recalcitrant Camellia drupifera capsules. [PDF]

Plant Methods
Shen H, Chen H, Li W, He S, Liao B, Xiong W, Shen Y, Li Y, Gao Y, Li YQ, Zhang B.   +10 more
europepmc   +1 more source

Reverse genetics construction and pathogenicity of a novel recombinant NADC30-like PRRSV isolated in China. [PDF]

Front Vet Sci
Guo J, Li C, Lu H, Wang B, Zhang L, Ding J, Jiao X, Li Q, Zhu S, Wang A, Li Y.   +10 more
europepmc   +1 more source

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Reverse genetics and human disease

Cell, 1986
openaire   +4 more sources

Forward in Reverse: How Reverse Genetics Complements Chemical Genetics

Pharmacogenomics, 2005
Pharmaceutical discovery has a renewed interest in physiological screening, while chemical genomics initiatives will soon provide a large amount of cellular assay data. However, there is no single robust approach to connect active compounds with their targets, limiting their experimental and therapeutic use.
openaire   +2 more sources

Genetics and reverse genetics of rotavirus

Current Opinion in Virology, 2012
Rotavirus is a member of the family Reoviridae, which have genomes consisting of 10-12 double-stranded RNA segments. The functions of proteins encoded by each segment of the rotavirus genome have been studied extensively by several methods including reassortants, temperature-sensitive mutants, isolates with rearranged RNA segments, RNAi analysis, and ...
Koki, Taniguchi, Satoshi, Komoto
openaire   +2 more sources

Reverse genetics in zebrafish

Physiological Genomics, 2000
Lekven, Arne C., Kathryn Ann Helde, Christopher J. Thorpe, Rebecca Rooke, and Randall T. Moon. Reverse genetics in zebrafish. Physiol Genomics 2: 37–48, 2000.—The zebrafish has become a popular model system for the study of vertebrate developmental biology because of its numerous strengths as a molecular genetic and embryological system.
A C, Lekven, K A, Helde, C J, Thorpe, R, Rooke, R T, Moon   +4 more
openaire   +2 more sources

Genetic engineering of rotaviruses by reverse genetics

Microbiology and Immunology, 2013
ABSTRACTThe rotavirus genome is composed of 11 gene segments of dsRNA. A recent breakthrough in the field of rotaviruses is the development of a reverse genetics system for generating recombinant rotaviruses possessing a gene segment derived from cloned cDNA.
Satoshi, Komoto, Koki, Taniguchi
openaire   +2 more sources

Reverse Genetics of Filoviruses

2017
Reverse genetics systems are used for the generation of recombinant viruses. For filoviruses, this technology has been available for more than 15 years and has been used to investigate questions regarding the molecular biology, pathogenicity, and host adaptation determinants of these viruses.
Thomas, Hoenen, Janine, Brandt, Yíngyún, Caì, Jens H, Kuhn, Courtney, Finch   +4 more
openaire   +2 more sources