Results 251 to 260 of about 1,328,460 (282)
Some of the next articles are maybe not open access.
Genetics and Reverse Genetics of Nidoviruses
2014The genomes of nidoviruses are infectious, and virus replication is initiated as the genome is delivered to the cytoplasm and the replicase is translated by the host cell ribosomes. Nidovirus reverse-genetics systems are needed to better understand aspects of their complex replication strategy, pathogenesis, and mechanisms of host range expansion, and ...
Damon J. Deming, Ralph S. Baric
openaire +1 more source
Reverse Genetics and Cystic Fibrosis
American Journal of Respiratory Cell and Molecular Biology, 1990Abstract The protein responsible for cystic fibrosis has been identified using an approach called “reverse” genetics. This approach relies on the chromosomal map position to direct the search for a disease gene, several novel cloning strategies to isolate the gene, and the gene's sequence to define the abnormal protein.
M C, Iannuzzi, F S, Collins
openaire +2 more sources
2020
This chapter focuses on reverse genetics in which the start point is the DNA sequence of a cloned gene, and the mutant phenotype—and hence biological function of the gene—is inferred from that sequence. The chapter starts with an exploration of the reverse genetic analysis carried out on budding yeast and mice.
openaire +1 more source
This chapter focuses on reverse genetics in which the start point is the DNA sequence of a cloned gene, and the mutant phenotype—and hence biological function of the gene—is inferred from that sequence. The chapter starts with an exploration of the reverse genetic analysis carried out on budding yeast and mice.
openaire +1 more source
Reverse Genetics of Influenza Viruses
2012The ability to modify influenza viruses at will has revolutionized influenza research. Reverse genetics has been used to generate mutant or reassortant influenza viruses to assess their replication, virulence, pathogenicity, host range, and transmissibility.
Gabriele, Neumann +2 more
openaire +2 more sources
Prions: Pathogenesis and Reverse Genetics
Annals of the New York Academy of Sciences, 2000Abstract: Spongiform encephalopathies are a group of infectious neurodegenerative diseases. The infectious agent that causes transmissible spongiform encephalopathies was termed prion by Stanley Prusiner. The prion hypothesis states that the partially protease‐resistant and detergent‐insoluble prion protein (PrPsc) is identical with the infectious ...
Aguzzi, A +8 more
openaire +4 more sources
Journal of Endocrinology, 1995
Introduction In mammals, sex is determined by the presence or absence of the Y chromosome. The first event in sexual differentiation is the differentiation of the bipotential gonad into testes or ovaries. All subsequent sexual differentiation is under hormonal control of the testes or ovaries. The genetic entity
openaire +2 more sources
Introduction In mammals, sex is determined by the presence or absence of the Y chromosome. The first event in sexual differentiation is the differentiation of the bipotential gonad into testes or ovaries. All subsequent sexual differentiation is under hormonal control of the testes or ovaries. The genetic entity
openaire +2 more sources
1999
Abstract The nematode Caenorhabditis elegans has about 19000 genes. Approximately 2000 of these have been identified in forward genetic screens for animals with mutant phenotypes. The generation and analysis of such mutants has been the key to the success of the C. elegans model because the in vivo function of a gene can be inferred from
openaire +1 more source
Abstract The nematode Caenorhabditis elegans has about 19000 genes. Approximately 2000 of these have been identified in forward genetic screens for animals with mutant phenotypes. The generation and analysis of such mutants has been the key to the success of the C. elegans model because the in vivo function of a gene can be inferred from
openaire +1 more source
Reverse Genetics for Mammalian Orthoreovirus
2017Reverse genetics allows introduction of specific alterations into a viral genome. Studies performed with mutant viruses generated using reverse genetics approaches have contributed immeasurably to our understanding of viral replication and pathogenesis, and also have led to development of novel vaccines and virus-based vectors.
Johnasha D, Stuart +2 more
openaire +2 more sources
Genetic programming for Reverse Engineering
2013 20th Working Conference on Reverse Engineering (WCRE), 2013This paper overviews the application of Search Based Software Engineering (SBSE) to reverse engineering with a particular emphasis on the growing importance of recent developments in genetic programming and genetic improvement for reverse engineering.
Mark Harman +2 more
openaire +1 more source