Results 251 to 260 of about 668,266 (290)
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Reverse Genetics of Influenza Viruses
2012The ability to modify influenza viruses at will has revolutionized influenza research. Reverse genetics has been used to generate mutant or reassortant influenza viruses to assess their replication, virulence, pathogenicity, host range, and transmissibility.
Gabriele, Neumann +2 more
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Reverse Genetics Using Transgenic Mice
Annual Review of Physiology, 1990n, has suggested a genetic approach in which the traditional logic is reversed. In this reverse genetic approach, a genotype is designed and constructed in vitro and implanted in the mouse germline by micro injection or by transfection into embryonic stem cells.
C P, Landel, S Z, Chen, G A, Evans
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Reverse Genetics of Zika Virus
2017We have established a reverse genetic system for Zika virus (ZIKV). Five shuttle plasmids were constructed and assembled into the full-length cDNA clone of ZIKV genome. To ensure the stability of the cDNA clone, we used a low copy vector (pACYC177) and a set of unique restriction enzyme sites on the ZIKV genome to assemble the full-length cDNA clone. A
Chao, Shan, Xuping, Xie, Pei-Yong, Shi
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Prions: Pathogenesis and Reverse Genetics
Annals of the New York Academy of Sciences, 2000Abstract: Spongiform encephalopathies are a group of infectious neurodegenerative diseases. The infectious agent that causes transmissible spongiform encephalopathies was termed prion by Stanley Prusiner. The prion hypothesis states that the partially protease‐resistant and detergent‐insoluble prion protein (PrPsc) is identical with the infectious ...
Aguzzi, A +8 more
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Reverse Genetics Systems for Filoviruses
2017Filoviruses are among the most pathogenic viruses known to man. Reverse genetics systems, in particular full-length clone systems, allow the generation of recombinant filoviruses, which can be used to study virus biology, but also for applied uses such as screening for countermeasures.
Thomas, Hoenen, Heinz, Feldmann
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Reverse Genetics for Mammalian Orthoreovirus
2017Reverse genetics allows introduction of specific alterations into a viral genome. Studies performed with mutant viruses generated using reverse genetics approaches have contributed immeasurably to our understanding of viral replication and pathogenesis, and also have led to development of novel vaccines and virus-based vectors.
Johnasha D, Stuart +2 more
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Chloroplast Transformation and Reverse Genetics
2006For chloroplast transformation, the most efficient method to introduce DNA is particle bombardment. To then select the cells which harbor a transformed plastid, two classes of markers are available. With one class, selection is based on the rescue of a non-photosynthetic mutant with the wild-type chloroplast gene.
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