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Genetics and reverse genetics of rotavirus
Current Opinion in Virology, 2012Rotavirus is a member of the family Reoviridae, which have genomes consisting of 10-12 double-stranded RNA segments. The functions of proteins encoded by each segment of the rotavirus genome have been studied extensively by several methods including reassortants, temperature-sensitive mutants, isolates with rearranged RNA segments, RNAi analysis, and ...
Koki, Taniguchi, Satoshi, Komoto
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Physiological Genomics, 2000
Lekven, Arne C., Kathryn Ann Helde, Christopher J. Thorpe, Rebecca Rooke, and Randall T. Moon. Reverse genetics in zebrafish. Physiol Genomics 2: 37–48, 2000.—The zebrafish has become a popular model system for the study of vertebrate developmental biology because of its numerous strengths as a molecular genetic and embryological system.
A C, Lekven +4 more
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Lekven, Arne C., Kathryn Ann Helde, Christopher J. Thorpe, Rebecca Rooke, and Randall T. Moon. Reverse genetics in zebrafish. Physiol Genomics 2: 37–48, 2000.—The zebrafish has become a popular model system for the study of vertebrate developmental biology because of its numerous strengths as a molecular genetic and embryological system.
A C, Lekven +4 more
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Genetic engineering of rotaviruses by reverse genetics
Microbiology and Immunology, 2013ABSTRACTThe rotavirus genome is composed of 11 gene segments of dsRNA. A recent breakthrough in the field of rotaviruses is the development of a reverse genetics system for generating recombinant rotaviruses possessing a gene segment derived from cloned cDNA.
Satoshi, Komoto, Koki, Taniguchi
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Reverse Genetics of Arenaviruses
2002Arenaviruses merit significant attention both as experimental models to study acute and persistent infections and as clinically important human pathogens, including hemorrhagic fever agents such as Lassa virus.
K J, Lee, J C, de la Torre
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Reverse Genetics of TheParamyxoviridae
1999Publisher Summary This chapter describes the experiments using the technology of nonsegmented negative-strand virus genome rescue in paramyxoviruses, which led to the recovery of infectious virus from complementary DNA (cDNA) and considers the rescue of infectious virus from the members of each of the four genera for which it has been achieved ...
A C, Marriott, A J, Easton
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Reverse Genetics of Nodaviruses
1999Publisher Summary This chapter focuses on the reverse genetics of Nodaviruses. With the construction of full-length complementary DNA (cDNA) clones of the two genomic RNAs of an alpha nodavirus, it became possible to address all stages of the virus life cycle using reverse genetic methods.
L A, Ball, K L, Johnson
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Forward in Reverse: How Reverse Genetics Complements Chemical Genetics
Pharmacogenomics, 2005Pharmaceutical discovery has a renewed interest in physiological screening, while chemical genomics initiatives will soon provide a large amount of cellular assay data. However, there is no single robust approach to connect active compounds with their targets, limiting their experimental and therapeutic use.
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Reverse Genetics of Dengue Virus
2023Dengue virus (DENV) is one of the most important and widespread arthropod-borne viruses, causing millions of infections over the years. Considering its epidemiological importance, efforts have been directed towards understanding various aspects of DENV biology, which have been facilitated by the development of different molecular strategies for ...
José Valter Joaquim, Silva Júnior +3 more
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Reverse Genetics of Picornaviruses
1999Publisher Summary This chapter focuses on the reverse genetics of picornaviruses. The family Picornaviridae includes a large number of human and animal pathogens, all related by the possession of a small, nonenveloped, icosahedral particle and a single-stranded, positive-sense, RNA genome.
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Reverse Genetics of Mononegavirales
2004"Reverse genetics" or de novo synthesis of nonsegmented negative-sense RNA viruses (Mononegavirales) from cloned cDNA has become a reliable technique to study this group of medically important viruses. Since the first generation of a negative-sense RNA virus entirely from cDNA in 1994, reverse genetics systems have been established for members of most ...
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