Results 241 to 250 of about 668,266 (290)
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2017
The Old World (OW) arenavirus Lassa (LASV ) is estimated to infect several hundred thousand people yearly in West Africa, resulting in high numbers of Lassa fever (LF), a viral hemorrhagic fever (HF) disease associated with high morbidity and mortality.
Luis, Martínez-Sobrido +2 more
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The Old World (OW) arenavirus Lassa (LASV ) is estimated to infect several hundred thousand people yearly in West Africa, resulting in high numbers of Lassa fever (LF), a viral hemorrhagic fever (HF) disease associated with high morbidity and mortality.
Luis, Martínez-Sobrido +2 more
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Reverse Genetics of Nodaviruses
1999Publisher Summary This chapter focuses on the reverse genetics of Nodaviruses. With the construction of full-length complementary DNA (cDNA) clones of the two genomic RNAs of an alpha nodavirus, it became possible to address all stages of the virus life cycle using reverse genetic methods.
L A, Ball, K L, Johnson
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2020
This chapter focuses on reverse genetics in which the start point is the DNA sequence of a cloned gene, and the mutant phenotype—and hence biological function of the gene—is inferred from that sequence. The chapter starts with an exploration of the reverse genetic analysis carried out on budding yeast and mice.
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This chapter focuses on reverse genetics in which the start point is the DNA sequence of a cloned gene, and the mutant phenotype—and hence biological function of the gene—is inferred from that sequence. The chapter starts with an exploration of the reverse genetic analysis carried out on budding yeast and mice.
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Reverse Genetics of Arenaviruses
2002Arenaviruses merit significant attention both as experimental models to study acute and persistent infections and as clinically important human pathogens, including hemorrhagic fever agents such as Lassa virus.
K J, Lee, J C, de la Torre
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Reverse Genetics Systems for Filoviruses
2023Filoviruses are causative agents of severe hemorrhagic fevers with high case fatality rates in humans. For studies of virus biology and the subsequent development of countermeasures, reverse genetic systems, and especially those facilitating the generation of recombinant filoviruses, are indispensable.
Bianca S, Bodmer, Thomas, Hoenen
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Reverse Genetics of TheParamyxoviridae
1999Publisher Summary This chapter describes the experiments using the technology of nonsegmented negative-strand virus genome rescue in paramyxoviruses, which led to the recovery of infectious virus from complementary DNA (cDNA) and considers the rescue of infectious virus from the members of each of the four genera for which it has been achieved ...
A C, Marriott, A J, Easton
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1999
Abstract The nematode Caenorhabditis elegans has about 19000 genes. Approximately 2000 of these have been identified in forward genetic screens for animals with mutant phenotypes. The generation and analysis of such mutants has been the key to the success of the C. elegans model because the in vivo function of a gene can be inferred from
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Abstract The nematode Caenorhabditis elegans has about 19000 genes. Approximately 2000 of these have been identified in forward genetic screens for animals with mutant phenotypes. The generation and analysis of such mutants has been the key to the success of the C. elegans model because the in vivo function of a gene can be inferred from
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Reverse Genetics of Picornaviruses
1999Publisher Summary This chapter focuses on the reverse genetics of picornaviruses. The family Picornaviridae includes a large number of human and animal pathogens, all related by the possession of a small, nonenveloped, icosahedral particle and a single-stranded, positive-sense, RNA genome.
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Reverse Genetics and Cystic Fibrosis
American Journal of Respiratory Cell and Molecular Biology, 1990The protein responsible for cystic fibrosis has been identified using an approach called "reverse" genetics. This approach relies on the chromosomal map position to direct the search for a disease gene, several novel cloning strategies to isolate the gene, and the gene's sequence to define the abnormal protein.
M C, Iannuzzi, F S, Collins
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Reverse Genetics of Mononegavirales
2004"Reverse genetics" or de novo synthesis of nonsegmented negative-sense RNA viruses (Mononegavirales) from cloned cDNA has become a reliable technique to study this group of medically important viruses. Since the first generation of a negative-sense RNA virus entirely from cDNA in 1994, reverse genetics systems have been established for members of most ...
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