Efficient generation of recombinant influenza A viruses employing a new approach to overcome the genetic instability of HA segments. [PDF]
PLoS ONE, 2015 Influenza A viruses (IAVs) are the most relevant and continual source of severe infectious respiratory complications in humans and different animal species, especially poultry.
Ahmed Mostafa +5 more
doaj +1 more source
Highly Efficient Site-Specific Mutagenesis in Malaria Mosquitoes Using CRISPR. [PDF]
, 2018 Anopheles mosquitoes transmit at least 200 million annual malaria infections worldwide. Despite considerable genomic resources, mechanistic understanding of biological processes in Anopheles has been hampered by a lack of tools for reverse genetics. Here,
Akbari, Omar S +2 more
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Rewriting Human History and Empowering Indigenous Communities with Genome Editing Tools. [PDF]
, 2020 Appropriate empirical-based evidence and detailed theoretical considerations should be used for evolutionary explanations of phenotypic variation observed in the field of human population genetics (especially Indigenous populations). Investigators within
Fox, Keolu +2 more
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Bacterial Genetic Engineering by Means of Recombineering for Reverse Genetics
Frontiers in Microbiology, 2020 Serving a robust platform for reverse genetics enabling the in vivo study of gene functions primarily in enterobacteriaceae, recombineering -or recombination-mediated genetic engineering-represents a powerful and relative straightforward genetic ...
Ursula Fels, K. Gevaert, P. Van Damme
semanticscholar +1 more source
Advantages, disadvantages and research progress on strategies for screening and identifying functional genes [PDF]
Jichu yixue yu linchuang, 2022 The screening and identification of functional genes is a key step to deeply understand various biological phenotypes. Forward genetics including traditional genetics, genome-wide association analysis, the comparison of gene expression profiles and ...
LIU Xu, CHANG De, WANG Jun-feng
doaj +1 more source
Porcine Reproductive and Respiratory Syndrome Virus Reverse Genetics and the Major Applications
Viruses, 2020 Porcine reproductive and respiratory syndrome virus (PRRSV) is a positive sense, single-stranded RNA virus that is known to infect only pigs. The virus emerged in the late 1980s and became endemic in most swine producing countries, causing substantial ...
Jayeshbhai Chaudhari, H. Vu
semanticscholar +1 more source
Cytokines, reverse genetics and anti-cytokine therapy
Бюллетень сибирской медицины, 2019 Cytokines comprise the molecular language of communication between the cells, which is needed to maintain the homeostatic functions of the body (including the immune system) and mediate various diseases.
M. S. Drutskaya +9 more
doaj +1 more source
Development of a minigenome cassette for Lettuce necrotic yellows virus: A first step in rescuing a plant cytorhabdovirus [PDF]
, 2020 Rhabdoviruses are enveloped negative-sense RNA viruses that have numerous biotechnological applications. However, recovering plant rhabdoviruses from cDNA remains difficult due to technical difficulties such as the need for concurrent in planta ...
Drake, P +3 more
core +1 more source
Journal of Virology, 2020 Although vaccines have reduced global RV-associated hospitalization and mortality over the past decade, the multisegmented genome of RVs allows reassortment of VP4 and VP7 genes from different RV species and strains. The evolutionary dynamics of novel RV
Y. Kanai +13 more
semanticscholar +1 more source
Reverse Genetics for Double-Stranded RNA Viruses
Revue d’Elevage et de Médecine Vétérinaire des Pays Tropicaux, 2009 Resumen en inglès, ver archivo ...
H. Attoui +3 more
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