Results 71 to 80 of about 2,173,029 (360)

Modern approaches to production of safe and effective genetically modified rabies vaccines for animals

open access: yesВетеринария сегодня, 2023
Rabies is a dangerous zoonoticdisease that affects the central nervous system, causes encephalomyelitis and paralyses and Is almost invariably fatal.
M. I. Doronin   +6 more
doaj   +1 more source

Development of Reverse Genetics for the Prototype New World Mammarenavirus Tacaribe Virus

open access: yesJournal of Virology, 2020
To date, no Food and Drug Administration (FDA)-approved vaccines are available to combat hemorrhagic fever caused by mammarenavirus infections in humans.
C. Ye   +2 more
semanticscholar   +1 more source

Evaluation of Stem-Loop Reverse Transcription and Poly-A Tail Extension in MicroRNA Analysis of Body Fluids [PDF]

open access: yes, 2014
MicroRNA has been demonstrated to be a viable tool for body fluid identification purposes in forensic casework. Stem-loop reverse transcription (slRT) is regularly used for cDNA synthesis from mature miRNA, along with poly-A tail extension.
Dunnett, Hannah   +2 more
core   +1 more source

Persistent Infection with a Rabbit Hepatitis E Virus Created by a Reverse Genetics System.

open access: yesTransboundary and Emerging Diseases, 2020
Rabbit hepatitis E virus (HEV) is a novel zoonotic infectious agent. Although a cell culture system to grow the virus has been established, there is currently no reverse genetics system for generating the virus.
Wenjing Zhang   +9 more
semanticscholar   +1 more source

Establishment of a Simple and Efficient Reverse Genetics System for Canine Adenoviruses Using Bacterial Artificial Chromosomes

open access: yesViruses, 2020
Canine adenoviruses (CAdVs) are divided into pathotypes CAdV1 and CAdV2, which cause infectious hepatitis and laryngotracheitis in canid animals, respectively.
Hiromichi Matsugo   +6 more
doaj   +1 more source

The human Cranio Facial Development Protein 1 (Cfdp1) gene encodes a protein required for the maintenance of higher-order chromatin organization [PDF]

open access: yes, 2017
The human Cranio Facial Development Protein 1 (Cfdp1) gene maps to chromosome 16q22.2-q22.3 and encodes the CFDP1 protein, which belongs to the evolutionarily conserved Bucentaur (BCNT) family.
Atterrato, Maria Teresa   +5 more
core   +3 more sources

RNA Virus Reverse Genetics and Vaccine Design

open access: yesViruses, 2014
RNA viruses are capable of rapid spread and severe or potentially lethal disease in both animals and humans. The development of reverse genetics systems for manipulation and study of RNA virus genomes has provided platforms for designing and optimizing ...
Christopher C. Stobart, Martin L. Moore
doaj   +1 more source

Rapid reconstruction of porcine reproductive and respiratory syndrome virus using synthetic DNA fragments

open access: yesComputational and Structural Biotechnology Journal, 2021
Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most challenging infectious disease of pig populations causing devastating economic loss to swine industry.
Julien Mélade   +6 more
doaj   +1 more source

Mutations in the Schmallenberg virus Gc glycoprotein facilitate cellular protein synthesis shutoff and restore pathogenicity of NSs deletion mutants in mice [PDF]

open access: yes, 2016
Serial passage of viruses in cell culture has been traditionally used to attenuate virulence and identify determinants of viral pathogenesis. In a previous study, we found that a strain of Schmallenberg virus (SBV) serially passaged in tissue culture ...
Aislynn Taggart   +13 more
core   +1 more source

Influenza reverse genetics: dissecting immunity and pathogenesis

open access: yes, 2014
Reverse genetics systems allow artificial generation of non-segmented and segmented negative-sense RNA viruses, like influenza viruses, entirely from cloned cDNA.
Evans, Justin G.   +2 more
core   +1 more source

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