Results 81 to 90 of about 2,173,029 (360)

Single-Round Infectious Particle Production by DNA-Launched Infectious Clones of Bungowannah Pestivirus

open access: yesViruses, 2020
Reverse genetics systems are powerful tools for functional studies of viral genes or for vaccine development. Here, we established DNA-launched reverse genetics for the pestivirus Bungowannah virus (BuPV), where cDNA flanked by a hammerhead ribozyme ...
Anja Dalmann   +5 more
doaj   +1 more source

One-Step Assembly of a Porcine Epidemic Diarrhea Virus Infectious cDNA Clone by Homologous Recombination in Yeast: Rapid Manipulation of Viral Genome With CRISPR/Cas9 Gene-Editing Technology

open access: yesFrontiers in Microbiology, 2022
Porcine epidemic diarrhea virus (PEDV), a swine enteric coronavirus causing acute diarrhea in piglets, is one of the major threatens to the pork industry globally.
Yanyang Zhou   +10 more
doaj   +1 more source

Genome editing enables reverse genetics of multicellular development in the choanoflagellate Salpingoeca rosetta

open access: yesbioRxiv, 2020
In a previous study, we established a forward genetic screen to identify genes required for multicellular development in the choanoflagellate, Salpingoeca rosetta (Levin et al., 2014).
David S. Booth, N. King
semanticscholar   +1 more source

Patient‐Level Barriers and Facilitators to Inpatient Physical Therapy in Adolescents and Young Adults With a Hematological Malignancy: A Qualitative Study

open access: yesPediatric Blood &Cancer, EarlyView.
ABSTRACT Background Despite their increased risk for functional impairment resulting from cancer and its treatments, few adolescents and young adults (AYAs) with a hematological malignancy receive the recommended or therapeutic dose of exercise per week during inpatient hospitalizations.
Jennifer A. Kelleher   +8 more
wiley   +1 more source

Occurrence of LINE, gypsy-like, and copia-like retrotransposons in the clonally propagated sweet potato (Ipomoea batatas L.) [PDF]

open access: yes, 2011
Retrotransposons are a class of transposable elements that represent a major fraction of the repetitive DNA of most eukaryotes. Their abundance stems from their expansive replication strategies.
Dieters, Mark   +3 more
core   +2 more sources

A Comparative Study of Cerebral Oxygenation During Exercise in Hemodialysis and Peritoneal Dialysis Patients

open access: yesTherapeutic Apheresis and Dialysis, EarlyView.
ABSTRACT Introduction Cognitive impairment and exercise intolerance are common in dialysis patients. Cerebral perfusion and oxygenation play a major role in both cognitive function and exercise execution; HD session per se aggravates cerebral ischemia in this population. This study aimed to compare cerebral oxygenation and perfusion at rest and in mild
Marieta P. Theodorakopoulou   +10 more
wiley   +1 more source

Development of a PCR-Based Reverse Genetics System for an Attenuated Duck Tembusu Virus Strain. [PDF]

open access: yesPLoS ONE, 2016
The infectious disease caused by the duck Tembusu virus (DTMUV) has resulted in massive economic losses to the Chinese duck industry in China since 2010.
Xiaogang Wu   +12 more
doaj   +1 more source

Reverse genetics system for human rotaviruses

open access: yesMicrobiology and immunology, 2020
A reverse genetics technology is an incredibly useful technique both for a proper understanding of different aspects of virus biology and for the generation of complementary DNA (cDNA)‐derived infectious viruses, which can act as safe and effective ...
S. Komoto   +3 more
semanticscholar   +1 more source

Mapping the evolution of mitochondrial complex I through structural variation

open access: yesFEBS Letters, EarlyView.
Respiratory complex I (CI) is crucial for bioenergetic metabolism in many prokaryotes and eukaryotes. It is composed of a conserved set of core subunits and additional accessory subunits that vary depending on the organism. Here, we categorize CI subunits from available structures to map the evolution of CI across eukaryotes. Respiratory complex I (CI)
Dong‐Woo Shin   +2 more
wiley   +1 more source

Modern Approaches to Molecular Genetics of Viruses in the Study of the Members of the Family Coronaviridae

open access: yesВестник войск РХБ защиты, 2021
The existence of certain suspicions about the artificial origin of the COVID-19 pandemic and about the possible use of reverse genetics technology to create the SARS-CoV-2 virus require an understanding of its capabilities in the design of new viruses ...
M. V. Supotnitskiy
doaj   +1 more source

Home - About - Disclaimer - Privacy