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Reverse genetics systems are powerful tools for functional studies of viral genes or for vaccine development. Here, we established DNA-launched reverse genetics for the pestivirus Bungowannah virus (BuPV), where cDNA flanked by a hammerhead ribozyme ...
Anja Dalmann +5 more
doaj +1 more source
Porcine epidemic diarrhea virus (PEDV), a swine enteric coronavirus causing acute diarrhea in piglets, is one of the major threatens to the pork industry globally.
Yanyang Zhou +10 more
doaj +1 more source
In a previous study, we established a forward genetic screen to identify genes required for multicellular development in the choanoflagellate, Salpingoeca rosetta (Levin et al., 2014).
David S. Booth, N. King
semanticscholar +1 more source
ABSTRACT Background Despite their increased risk for functional impairment resulting from cancer and its treatments, few adolescents and young adults (AYAs) with a hematological malignancy receive the recommended or therapeutic dose of exercise per week during inpatient hospitalizations.
Jennifer A. Kelleher +8 more
wiley +1 more source
Occurrence of LINE, gypsy-like, and copia-like retrotransposons in the clonally propagated sweet potato (Ipomoea batatas L.) [PDF]
Retrotransposons are a class of transposable elements that represent a major fraction of the repetitive DNA of most eukaryotes. Their abundance stems from their expansive replication strategies.
Dieters, Mark +3 more
core +2 more sources
ABSTRACT Introduction Cognitive impairment and exercise intolerance are common in dialysis patients. Cerebral perfusion and oxygenation play a major role in both cognitive function and exercise execution; HD session per se aggravates cerebral ischemia in this population. This study aimed to compare cerebral oxygenation and perfusion at rest and in mild
Marieta P. Theodorakopoulou +10 more
wiley +1 more source
Development of a PCR-Based Reverse Genetics System for an Attenuated Duck Tembusu Virus Strain. [PDF]
The infectious disease caused by the duck Tembusu virus (DTMUV) has resulted in massive economic losses to the Chinese duck industry in China since 2010.
Xiaogang Wu +12 more
doaj +1 more source
Reverse genetics system for human rotaviruses
A reverse genetics technology is an incredibly useful technique both for a proper understanding of different aspects of virus biology and for the generation of complementary DNA (cDNA)‐derived infectious viruses, which can act as safe and effective ...
S. Komoto +3 more
semanticscholar +1 more source
Mapping the evolution of mitochondrial complex I through structural variation
Respiratory complex I (CI) is crucial for bioenergetic metabolism in many prokaryotes and eukaryotes. It is composed of a conserved set of core subunits and additional accessory subunits that vary depending on the organism. Here, we categorize CI subunits from available structures to map the evolution of CI across eukaryotes. Respiratory complex I (CI)
Dong‐Woo Shin +2 more
wiley +1 more source
The existence of certain suspicions about the artificial origin of the COVID-19 pandemic and about the possible use of reverse genetics technology to create the SARS-CoV-2 virus require an understanding of its capabilities in the design of new viruses ...
M. V. Supotnitskiy
doaj +1 more source

