Relevance of micrometastases detected by reverse transcriptase-polymerase chain reaction for melanoma recurrence: systematic review and meta-analysis [PDF]
CONTEXT: Cutaneous melanoma presents significant morbidity and mortality. Nowadays, about 90% of them are diagnosed by clinical examination and most are localized melanomas.
Allisson Monteiro da Silva +3 more
doaj +3 more sources
In-house reverse transcriptase polymerase chain reaction for detection of SARS-CoV-2 with increased sensitivity [PDF]
As the COVID-19 infection continues to ravage the world, the advent of an efficient as well as the economization of the existing RT-PCR based detection assay essentially can become a blessing in these testing times and significantly help in the ...
Manash Jyoti Kalita +12 more
doaj +3 more sources
COVID-19 Diagnosed by Real-Time Reverse Transcriptase-Polymerase Chain Reaction in Nasopharyngeal Specimens of Suspected Cases in a Tertiary Care Center: A Descriptive Cross-sectional Study [PDF]
Introduction: The emergence of severe acute respiratory syndrome coronavirus-2 pandemic is critically challenging the whole world. The real-time reverse transcriptase-polymerase chain reaction is the most widely used confirmatory test for COVID-19 ...
Narayani Maharjan +4 more
doaj +2 more sources
Diagnosis of Dengue by Using Reverse Transcriptase-Polymerase Chain Reaction [PDF]
A rapid identification of dengue viruses from clinical samples by using a nested reverse transcriptase-polymerase chain reaction (RT-PCR) procedure was carried out for diagnostic and epidemiological purposes.
Marize Pereira Miagostovich +5 more
doaj +3 more sources
Direct in situ reverse transcriptase-polymerase chain reaction [PDF]
In situ hybridization has been used for localization of specific nucleic acid sequences at the cellular level despite providing relatively low-detection sensitivity. In situ reverse transcriptase-polymerase chain reactions (RT-PCR) enhance sensitivity and thus enable localization of low-abundance mRNA in a cell.
Rajesh Kher, Robert L. Bacallao
openalex +3 more sources
Enteroviral pharyngitis diagnosed by reverse transcriptase-polymerase chain reaction. [PDF]
The role of enteroviruses in childhood pharyngitis was investigated using enteroviral specific reverse transcriptase-polymerase chain reaction (RT-PCR). Viral/bacterial throat swabs were taken from 50 children with acute pharyngitis and 26 controls.
Mike Sharland +4 more
+6 more sources
Newcastle Disease Virus Detection from Chicken Organ Samples Using Reverse Transcriptase Polymerase Chain Reaction [PDF]
Newcastle disease (ND) is a systemic, viral respiratory disease that is acute and easily transmitted which affects various types of poultry, especially chickens.
Lehgarubini Shanmuganathan +2 more
doaj +6 more sources
Molecularly imprinted nanoparticles for inhibiting ribonuclease in reverse transcriptase polymerase chain reaction [PDF]
We showed that molecularly imprinted nanoparticles (nanoMIPs) could efficiently inhibit the activities of the RNase in RT-PCR reactions, demonstrating that the tailor-made nanomaterials are very promising for use in routine biological assays.
Xiaotong Feng +4 more
openalex +7 more sources
Diagnostic Accuracy of SARS-CoV-2 Nucleocapsid Antigen Self-Test in Comparison to Reverse Transcriptase-Polymerase Chain Reaction. [PDF]
Sukumaran A +9 more
europepmc +3 more sources
Standardization and validation of a novel reverse transcriptase polymerase chain reaction method for detecting virulent strains of the infectious bursal disease virus. [PDF]
Longa-Bobadilla V +8 more
europepmc +2 more sources

