Results 1 to 10 of about 171,614 (301)

In-house reverse transcriptase polymerase chain reaction for detection of SARS-CoV-2 with increased sensitivity [PDF]

Scientific Reports, 2021
As the COVID-19 infection continues to ravage the world, the advent of an efficient as well as the economization of the existing RT-PCR based detection assay essentially can become a blessing in these testing times and significantly help in the ...
Manash Jyoti Kalita, Kalpajit Dutta, Gautam Hazarika, Ridip Dutta, Simanta Kalita, Partha Pratim Das, Manash P. Sarma, Sofia Banu, Md. Ghaznavi Idris, Anjan Jyoti Talukdar, Sangitanjan Dutta, Ajanta Sharma, Subhash Medhi   +12 more
doaj   +3 more sources

COVID-19 Diagnosed by Real-Time Reverse Transcriptase-Polymerase Chain Reaction in Nasopharyngeal Specimens of Suspected Cases in a Tertiary Care Center: A Descriptive Cross-sectional Study [PDF]

Journal of Nepal Medical Association, 2021
Introduction: The emergence of severe acute respiratory syndrome coronavirus-2 pandemic is critically challenging the whole world. The real-time reverse transcriptase-polymerase chain reaction is the most widely used confirmatory test for COVID-19 ...
Narayani Maharjan, Niresh Thapa, Bibek Pun Magar, Muna Maharjan, Jiancheng Tu   +4 more
doaj   +2 more sources

Diagnosis of Dengue by Using Reverse Transcriptase-Polymerase Chain Reaction [PDF]

Memorias do Instituto Oswaldo Cruz, 1997
A rapid identification of dengue viruses from clinical samples by using a nested reverse transcriptase-polymerase chain reaction (RT-PCR) procedure was carried out for diagnostic and epidemiological purposes.
Marize Pereira Miagostovich, Flávia Barreto dos Santos, Eliane Saraiva M de Araújo, Juarez Dias, Hermann G Schatzmayr, Rita Maria Ribeiro Nogueira   +5 more
doaj   +3 more sources

Enteroviral pharyngitis diagnosed by reverse transcriptase-polymerase chain reaction [PDF]

International Journal of Pediatric Otorhinolaryngology, 1996
The role of enteroviruses in childhood pharyngitis was investigated using enteroviral specific reverse transcriptase-polymerase chain reaction (RT-PCR). Viral/bacterial throat swabs were taken from 50 children with acute pharyngitis and 26 controls.
M, Sharland, J, Hodgson, E G, Davies, J, Booth, S, Jeffery   +4 more
  +6 more sources

Direct in situ reverse transcriptase-polymerase chain reaction [PDF]

American Journal of Physiology-Cell Physiology, 2001
In situ hybridization has been used for localization of specific nucleic acid sequences at the cellular level despite providing relatively low-detection sensitivity. In situ reverse transcriptase-polymerase chain reactions (RT-PCR) enhance sensitivity and thus enable localization of low-abundance mRNA in a cell.
R, Kher, R, Bacallao
openaire   +3 more sources

Molecularly imprinted nanoparticles for inhibiting ribonuclease in reverse transcriptase polymerase chain reaction [PDF]

The Analyst, 2018
We showed that molecularly imprinted nanoparticles (nanoMIPs) could efficiently inhibit the activities of the RNase in RT-PCR reactions, demonstrating that the tailor-made nanomaterials are very promising for use in routine biological assays.
Xiaotong Feng, Jon Ashley, Tongchang Zhou, Arnab Halder, Yi Sun   +4 more
openaire   +7 more sources

Diagnostic Accuracy of SARS-CoV-2 Nucleocapsid Antigen Self-Test in Comparison to Reverse Transcriptase-Polymerase Chain Reaction. [PDF]

J Appl Lab Med, 2022
Sukumaran A, Suvekbala V, R AK, Thomas RE, Raj A, Thomas T, Abhijith BL, Jose J, Paul JK, Vasudevan DM.   +9 more
europepmc   +3 more sources

Standardization and validation of a novel reverse transcriptase polymerase chain reaction method for detecting virulent strains of the infectious bursal disease virus. [PDF]

Vet World
Longa-Bobadilla V, Ormeño-Vásquez P, Criollo-Orozco M, Tataje-Lavanda L, Huamán-Gutierrez K, Montalván Á, Zimic M, Fernández-Sanchez M, Fernández-Díaz M.   +8 more
europepmc   +3 more sources

S-gene Dropout and False-negative Reverse Transcriptase-polymerase Chain Reaction Tests. [PDF]

Ann Maxillofac Surg, 2021
Balaji SM.
europepmc   +3 more sources

Pengembangan Sejumlah Primer untuk Reverse Transcriptase Polymerase Chain Reaction Guna Melacak Virus Flu Burung di Indonesia (DEVELOPMENt OF PRIMERS FOR REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION TO DETECT AVIAN INFLUENZA VIRUS IN INDONESIA)

Jurnal Veteriner, 2016
Until recently, two clades of of avian influenza viruses (AIVs) designated as 2.3.2 and 2.2.3 havebeen circulating in Indonesia. Mutations of AIV genes have cretaed many more variants of the virus.
Ni Luh Putu Indi Dharmayanti, Risza Hartawan, Dyah Ayu Hewajuli   +2 more
doaj   +1 more source