Diagnosis of Dengue by Using Reverse Transcriptase-Polymerase Chain Reaction [PDF]
Memorias do Instituto Oswaldo Cruz, 1997 A rapid identification of dengue viruses from clinical samples by using a nested reverse transcriptase-polymerase chain reaction (RT-PCR) procedure was carried out for diagnostic and epidemiological purposes.
Marize Pereira Miagostovich +5 more
doaj +3 more sources
A real-time reverse transcriptase polymerase chain reaction for detection and quantification of Vesiculovirus [PDF]
Memorias do Instituto Oswaldo Cruz, 2016 Vesiculoviruses (VSV) are zoonotic viruses that cause vesicular stomatitis disease in cattle, horses and pigs, as well as sporadic human cases of acute febrile illness. Therefore, diagnosis of VSV infections by reliable laboratory techniques is important
Aline Lavado Tolardo +14 more
doaj +3 more sources
Enteroviral pharyngitis diagnosed by reverse transcriptase-polymerase chain reaction. [PDF]
International Journal of Pediatric Otorhinolaryngology, 1996 The role of enteroviruses in childhood pharyngitis was investigated using enteroviral specific reverse transcriptase-polymerase chain reaction (RT-PCR). Viral/bacterial throat swabs were taken from 50 children with acute pharyngitis and 26 controls.
Mike Sharland +4 more
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Detection of chicken astrovirus by reverse transcriptase-polymerase chain reaction [PDF]
Avian Pathology, 2009 The development of a reverse transcriptase-polymerase chain reaction (RT-PCR) test for detecting chicken astroviruses (CAstV) is described. Primers, which amplified a fragment of 510 base pairs, were located in conserved regions within the ORF 1b (RNA polymerase) gene. The limit of detection of the test was estimated to be approximately 60 viral copies
V. J. Smyth +3 more
openalex +3 more sources
Outcomes of symptom screening and universal COVID-19 reverse transcriptase polymerase chain reaction testing before endoscopy in a community-based ambulatory surgery center. [PDF]
Gastrointest Endosc, 2021 Bowyer B +7 more
europepmc +3 more sources
The comparison of chest CT and RT-PCR during the diagnosis of COVID-19 [PDF]
Ķazaķstannyṇ Klinikalyķ Medicinasy, 2021 Objective: Our aim in this study is to compare the diagnostic accuracy of chest computed tomography and Real-time Reverse Transcriptase Polymerase Chain Reaction in a population with suspected Coronavirus Disease 2019 (COVID-19) presenting at the ...
Avni Uygar Seyhan +5 more
doaj +1 more source
S-gene Dropout and False-negative Reverse Transcriptase-polymerase Chain Reaction Tests. [PDF]
Ann Maxillofac Surg, 2021 Balaji SM.
europepmc +3 more sources
Journal of Nobel Medical College, 2022 Background: Corona Virus Disease 2019came into existence in December 2019, as a sudden outbreak inthe city of Wuhan, China. Early identification of the patient is vitalto control disease transmission.This study is aimed at estimatingthe sensitivity ...
Robinson Shrestha, Mahesh Gautam
doaj +1 more source
Reverse-Transcriptase Polymerase Chain Reaction to Detect Extracellular mRNAs [PDF]
, 2011 The presence of extracellular nucleic acids has been reported in serum/plasma from cancer and diabetes patients that may help in disease diagnosis. Taking insulin-producing cells as examples here, RT-PCR was used to investigate a correlation between the presence and amounts of extracellular mRNA(s) and cell mass and/or function. RT-PCR was performed on
Sweta Rani, Lorraine O’Driscoll
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Utilization of a deoxynucleoside diphosphate substrate by HIV reverse transcriptase [PDF]
, 2008 Background: Deoxynucleoside triphosphates (dNTPs) are the normal substrates for DNA sysnthesis is catalyzed by polymerases such as HIV-1 reverse transcriptase (RT).
A Bevington +53 more
core +15 more sources