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Newcastle Disease Virus Detection from Chicken Organ Samples Using Reverse Transcriptase Polymerase Chain Reaction [PDF]

Jurnal Sain Veteriner, 2017
Newcastle disease (ND) is a systemic, viral respiratory disease that is acute and easily transmitted which affects various types of poultry, especially chickens.
Lehgarubini Shanmuganathan, Dito Anggoro, Michael Haryadi Wibowo   +2 more
doaj   +6 more sources

Generation of non-genomic oligonucleotide tag sequences for RNA template-specific PCR [PDF]

BMC Biotechnology, 2006
Background In order to overcome genomic DNA contamination in transcriptional studies, reverse template-specific polymerase chain reaction, a modification of reverse transcriptase polymerase chain reaction, is used.
Lindblad Peter, Svensson Håkan, Pinto Fernando   +2 more
doaj   +4 more sources

West Nile Virus Detection in Urine [PDF]

Emerging Infectious Diseases, 2005
We report West Nile virus (WNV) RNA in urine collected from a patient with encephalitis 8 days after symptom onset. Viral RNA was detected by reverse transcriptase–polymerase chain reaction (RT-PCR).
Jessica H. Tonry, Craig B. Brown, Cecil B. Cropp, Juliene K.G. Co, Shannon N. Bennett, Vivek R. Nerurkar, Timothy Kuberski, Duane J. Gubler   +7 more
doaj   +3 more sources

Living with COVID-19: could SARS-CoV-2 infection present a ping-pong effect? [PDF]

, 2021
The epidemic of a novel coronavirus, named as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is still gradually spreading worldwide. Real-time fluorescence quantitative reverse transcriptase polymerase chain reaction (reverse transcriptase-
Ambrosio, Alessandro, Baffetti, Francesca Romana, Catalano, Carlo, Di Nunno, Donato, Laghi, Alessandro, Mammana, Giacomo, Marseglia, Bernardino, Montini, Antonio, Panichella, Nazareno   +8 more
core   +2 more sources

Experience With Pretravel Testing for SARS-CoV-2 at an Academic Medical Center

Academic Pathology, 2021
International travel has been a significant factor in the coronavirus disease 2019 pandemic. Many countries and airlines have implemented travel restrictions to limit the spread of the causative agent, severe acute respiratory syndrome coronavirus-2.
Katherine L. Imborek MD, Matthew D. Krasowski MD, PhD, Paul Natvig MD, Anna E. Merrill PhD, Daniel J. Diekema MD, Bradley A. Ford MD, PhD   +5 more
doaj   +1 more source

Utilization of a deoxynucleoside diphosphate substrate by HIV reverse transcriptase [PDF]

, 2008
Background: Deoxynucleoside triphosphates (dNTPs) are the normal substrates for DNA sysnthesis is catalyzed by polymerases such as HIV-1 reverse transcriptase (RT).
A Bevington, A Mas, AJ Smith, B Selmi, BA Barshop, BA Mulder, BR Bochner, C Cruchaga, C Isnard Bagnis, CK Tan, D Arion, EF Pettersen, G Pontarin, G Pontarin, G Yang, G Yang, GJ Kemp, H Huang, H Ling, H Pelletier, H Pelletier, HK Schachman, IA Nazarenko, K Bebenek, KL White, Kornberg, Linqi Zhang, M Rigourd, MC Franklin, Michael A. Parniak, MJ Bessman, MK Kukhanova, N Sluis-Cremer, PJ Rothwell, PL Boyer, PM Burgers, PM Burgers, PR Meyer, PR Meyer, PR Meyer, PR Meyer, PR Meyer, RS Fletcher, S Dharmasena, Scott J. Garforth, SJ Garforth, SV Doronin, SV Doronin, TA Kunkel, TA Rozovskaya, TA Steitz, UM Parikh, Vinayaka R. Prasad, Z Gu   +53 more
core   +14 more sources

Direct in situ reverse transcriptase-polymerase chain reaction [PDF]

American Journal of Physiology-Cell Physiology, 2001
In situ hybridization has been used for localization of specific nucleic acid sequences at the cellular level despite providing relatively low-detection sensitivity. In situ reverse transcriptase-polymerase chain reactions (RT-PCR) enhance sensitivity and thus enable localization of low-abundance mRNA in a cell.
Robert L. Bacallao, Rajesh Kher
openaire   +3 more sources

Accuracy of Rapid Antigen Detection Test Device (COVID-19 Ag Rapid Test Device) in Diagnosis of Acute COVID-19 Infections

Pakistan Armed Forces Medical Journal, 2022
Objective: To evaluate the accuracy of the COVID-19 Ag Rapid test device for detection of SARS-CoV-2 with Reverse Transcriptase Polymerase Chain Reaction as the gold standard. Study Design: Cross-sectional study.
Noman Shakoor, Eijaz Ghani, Saifullah Khan Niazi, Misbah Noor, Faraz Ahmed, Hammad Hussain   +5 more
doaj   +1 more source

REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION ASSAYS FOR PROSTATE CANCER [PDF]

Urologic Clinics of North America, 1997
RT-PCR, if targeted against a prostate-specific marker, can be a highly specific and sensitive assay to detect the presence of occult prostate cancer cells at sites far distant from the primary tumor. The low false-positive rate (0.8%) observed when combining most published studies and the high rate of detection of prostate cells in metastatic patients
Glen M. de Vries, Aaron E. Katz, Ralph Buttyan, Carl A. Olsson   +3 more
openaire   +2 more sources

Long term persistence of coronavirus SARS-CoV-2 infection

Einstein (São Paulo), 2021
During the COVID-19 pandemic, a case of a long-term persistence of SARS-CoV-2 infection (from March 26 to May 20, 2020) was identified at a private hospital in São Paulo, SP, Brazil.
João Renato Rebello Pinho, Ketti Gleyzer de Oliveira, Roberta Sitnik, Maira Marranghello Maluf, Pedro Henrique Sebe Rodrigues, Rúbia Anita Ferraz Santana, Eliane Rosseto Welter, Ophir Irony   +7 more
doaj   +2 more sources