Results 21 to 30 of about 515,505 (407)
Detection of chicken astrovirus by reverse transcriptase-polymerase chain reaction [PDF]
The development of a reverse transcriptase-polymerase chain reaction (RT-PCR) test for detecting chicken astroviruses (CAstV) is described. Primers, which amplified a fragment of 510 base pairs, were located in conserved regions within the ORF 1b (RNA polymerase) gene. The limit of detection of the test was estimated to be approximately 60 viral copies
V. Smyth+3 more
semanticscholar +4 more sources
This case series examines patients with coronavirus disease 2019 (COVID-19) who were discharged from the hospital and had recurrent positive reverse transcriptase–polymerase chain reaction (RT-PCR) results.
Hu R+6 more
europepmc +2 more sources
Generation of non-genomic oligonucleotide tag sequences for RNA template-specific PCR [PDF]
Background In order to overcome genomic DNA contamination in transcriptional studies, reverse template-specific polymerase chain reaction, a modification of reverse transcriptase polymerase chain reaction, is used.
Lindblad Peter+2 more
doaj +4 more sources
West Nile Virus Detection in Urine [PDF]
We report West Nile virus (WNV) RNA in urine collected from a patient with encephalitis 8 days after symptom onset. Viral RNA was detected by reverse transcriptase–polymerase chain reaction (RT-PCR).
Jessica H. Tonry+7 more
doaj +3 more sources
West Nile Virus Encephalitis in a Barbary Macaque (Macaca sylvanus) [PDF]
An aged Barbary ape (Macaca sylvanus) at the Toronto Zoo became infected with naturally acquired West Nile virus (WNV) encephalitis that caused neurologic signs, which, associated with other medical problems, led to euthanasia. The diagnosis was based on
Rolf-Arne Ølberg+5 more
doaj +3 more sources
Newcastle Disease Virus Detection from Chicken Organ Samples Using Reverse Transcriptase Polymerase Chain Reaction [PDF]
Newcastle disease (ND) is a systemic, viral respiratory disease that is acute and easily transmitted which affects various types of poultry, especially chickens.
Lehgarubini Shanmuganathan+2 more
doaj +6 more sources
Possible Central Nervous System Infection by SARS Coronavirus [PDF]
On day 22 of illness, generalized tonic-clonic convulsion developed in a 32-year-old woman with severe acute respiratory syndrome (SARS). Cerebrospinal fluid tested positive for SARS coronavirus (SARS-CoV) by reverse transcriptase–polymerase chain ...
Kwok-Kwong Lau+5 more
doaj +4 more sources
Norwalk-Like Viral Gastroenteritis Outbreak in U.S. Army Trainees [PDF]
An outbreak of acute gastroenteritis hospitalized 99 (12%) of 835 U.S. Army trainees at Fort Bliss, El Paso, Texas, rom August 27 to September 1, 1998.
Mark K. Arness+13 more
doaj +3 more sources
A reverse transcriptase‐polymerase chain reaction assay in the diagnosis of soft tissue sarcomas [PDF]
Many types of sarcomas are characterized by specific chromosomal translocations that result in the production of novel chimeric genes. Detection of these fusion genes could be a sensitive molecular diagnostic assay. However, to the authors' knowledge there have been few systemic comparisons between the current histopathologic diagnosis and the presence
Noriko Naito+7 more
openalex +4 more sources
Outbreak of West Nile Virus Infection, Volgograd Region, Russia, 1999 [PDF]
From July 25 to October 1, 1999, 826 patients were admitted to Volgograd Region, Russia, hospitals with acute aseptic meningoencephalitis, meningitis, or fever consistent with arboviral infection.
Alexander E. Platonov+11 more
doaj +3 more sources