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Detection of chicken astrovirus by reverse transcriptase-polymerase chain reaction [PDF]
Avian Pathology, 2009 The development of a reverse transcriptase-polymerase chain reaction (RT-PCR) test for detecting chicken astroviruses (CAstV) is described. Primers, which amplified a fragment of 510 base pairs, were located in conserved regions within the ORF 1b (RNA polymerase) gene. The limit of detection of the test was estimated to be approximately 60 viral copies
B. M. Adair +3 more
openaire +3 more sources
Acute viral gastroenteritis in children hospitalized in Iksan, Korea during December 2010-June 2011 [PDF]
Korean Journal of Pediatrics, 2013 PurposeViral etiology is common in cases of children with acute diarrhea, and antibiotic therapy is usually not required. Therefore, it is important to determine the distribution of common viruses among children hospitalized with acute diarrhea.MethodsWe
Cheol Whoan So +4 more
doaj +1 more source
Enteroviral pharyngitis diagnosed by reverse transcriptase-polymerase chain reaction. [PDF]
Archives of Disease in Childhood, 1996 The role of enteroviruses in childhood pharyngitis was investigated using enteroviral specific reverse transcriptase-polymerase chain reaction (RT-PCR). Viral/bacterial throat swabs were taken from 50 children with acute pharyngitis and 26 controls.
J Hodgson +4 more
openaire +3 more sources
Reverse-Transcriptase Polymerase Chain Reaction to Detect Extracellular mRNAs [PDF]
, 2011 The presence of extracellular nucleic acids has been reported in serum/plasma from cancer and diabetes patients that may help in disease diagnosis. Taking insulin-producing cells as examples here, RT-PCR was used to investigate a correlation between the presence and amounts of extracellular mRNA(s) and cell mass and/or function. RT-PCR was performed on
Lorraine O'Driscoll, Sweta Rani
openaire +5 more sources
Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) and its usefulness in soil microbial ecological studies - A review [PDF]
, 2014 The reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) is a highly specific polymerase chain reaction (PCR) method that allows one to detect very low transcription levels of functional gene(s) in soil.
Asuming-Brempong, S
core +2 more sources
Academic Pathology, 2021 Molecular techniques, especially reverse transcriptase polymerase chain reaction (RT-PCR), have been the gold standard for the diagnosis of acute severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection.
Robert M. Humble MD +4 more
doaj +1 more source
Molecular diagnosis of peste des petits ruminants virus (PPR) in goats and sheep populations [PDF]
, 2022 Peste des petits ruminants (PPR) is an economically important viral disease of goats and sheep. The disease is confused clinically with other infections such as the mild strain of rinderpest in small ruminants.
Abdel-Rady, A
core +1 more source
What are we missing? Three cases of severe COVID-19 pneumonia with negative testing
SAGE Open Medical Case Reports, 2020 The COVID-19 pandemic has drastically affected health care systems globally. Reverse transcriptase-polymerase chain reaction is currently the preferred method of detecting COVID-19; however, sensitivity of this test remains questionable.
Aisha Siddiqui +5 more
doaj +1 more source
The Iraqi Journal of Agricultural science, 2020 This study was aimed to highlight the importance of the melt curve-quantitative reverse transcriptase PCR (RT-qPCR) analysis in the detection of astrovirus (AstV) from both negative and positive rotavirus and enterovirus (EVs) samples, and the ...
Fadhil & et al.
doaj +1 more source
Molecularly imprinted nanoparticles for inhibiting ribonuclease in reverse transcriptase polymerase chain reaction [PDF]
The Analyst, 2018 We showed that molecularly imprinted nanoparticles (nanoMIPs) could efficiently inhibit the activities of the RNase in RT-PCR reactions, demonstrating that the tailor-made nanomaterials are very promising for use in routine biological assays.
Xiaotong Feng +4 more
openaire +6 more sources