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Long term persistence of coronavirus SARS-CoV-2 infection

open access: yesEinstein (São Paulo), 2021
During the COVID-19 pandemic, a case of a long-term persistence of SARS-CoV-2 infection (from March 26 to May 20, 2020) was identified at a private hospital in São Paulo, SP, Brazil.
João Renato Rebello Pinho   +7 more
doaj   +2 more sources

Acute viral gastroenteritis in children hospitalized in Iksan, Korea during December 2010-June 2011 [PDF]

open access: yesKorean Journal of Pediatrics, 2013
PurposeViral etiology is common in cases of children with acute diarrhea, and antibiotic therapy is usually not required. Therefore, it is important to determine the distribution of common viruses among children hospitalized with acute diarrhea.MethodsWe
Cheol Whoan So   +4 more
doaj   +1 more source

Detection of chicken astrovirus by reverse transcriptase-polymerase chain reaction [PDF]

open access: yesAvian Pathology, 2009
The development of a reverse transcriptase-polymerase chain reaction (RT-PCR) test for detecting chicken astroviruses (CAstV) is described. Primers, which amplified a fragment of 510 base pairs, were located in conserved regions within the ORF 1b (RNA polymerase) gene. The limit of detection of the test was estimated to be approximately 60 viral copies
V J, Smyth   +3 more
openaire   +2 more sources

Practical Considerations for Implementation of SARS-CoV-2 Serological Testing in the Clinical Laboratory: Experience at an Academic Medical Center

open access: yesAcademic Pathology, 2021
Molecular techniques, especially reverse transcriptase polymerase chain reaction (RT-PCR), have been the gold standard for the diagnosis of acute severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection.
Robert M. Humble MD   +4 more
doaj   +1 more source

Correlation between CSF biomarkers and COVID-19 meningoencephalitis: A case series

open access: yesRespiratory Medicine Case Reports, 2021
Background: Recent studies have focused on the incidence rate and pattern of meningoencephalitis in the coronavirus disease 2019 (COVID-19). Aim: This study aims to shed more light on the CSF pattern and clinical characteristics of meningoencephalitis ...
Minoosh Moghimi   +5 more
doaj   +1 more source

MOLECULAR DETECTION OF ASTROVIRUS USING REVERSE TRANSCRIPTASE QUANTITATIVE POLYMERASE CHAIN REACTION TECHNIQUE

open access: yesThe Iraqi Journal of Agricultural science, 2020
This study was aimed to highlight the importance of the melt curve-quantitative reverse transcriptase PCR (RT-qPCR) analysis in the detection of astrovirus (AstV) from both negative and positive rotavirus and enterovirus (EVs) samples, and the ...
Fadhil & et al.
doaj   +1 more source

What are we missing? Three cases of severe COVID-19 pneumonia with negative testing

open access: yesSAGE Open Medical Case Reports, 2020
The COVID-19 pandemic has drastically affected health care systems globally. Reverse transcriptase-polymerase chain reaction is currently the preferred method of detecting COVID-19; however, sensitivity of this test remains questionable.
Aisha Siddiqui   +5 more
doaj   +1 more source

Genetic diversity among viruses associated with sugarcane mosaic disease in Tucumán, Argentina [PDF]

open access: yes, 2009
Sugarcane leaves with mosaic symptoms were collected in 2006--07 in Tucumán (Argentina) and analyzed by reverse-transcriptase polymerase chain reaction (RT-PCR) restriction fragment length polymorphism (RFLP) and sequencing of a fragment of the Sugarcane
Castagnaro, Atilio Pedro   +6 more
core   +2 more sources

Expanding molecular diagnostic capacity for COVID-19 in Ethiopia: operational implications, challenges and lessons learnt

open access: yesThe Pan African Medical Journal, 2021
Efforts towards slowing down coronavirus (COVID-19) transmission and reducing mortality have focused on timely case detection, isolation and treatment.
Adisu Kebede   +17 more
doaj   +1 more source

Reverse-Transcriptase Polymerase Chain Reaction to Detect Extracellular mRNAs [PDF]

open access: yes, 2011
The presence of extracellular nucleic acids has been reported in serum/plasma from cancer and diabetes patients that may help in disease diagnosis. Taking insulin-producing cells as examples here, RT-PCR was used to investigate a correlation between the presence and amounts of extracellular mRNA(s) and cell mass and/or function. RT-PCR was performed on
Sweta, Rani, Lorraine, O'Driscoll
openaire   +4 more sources

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