Results 31 to 40 of about 973,723 (161)

Current approaches to development of real-time qPCR test-kits

Ветеринария сегодня, 2023
Currently fluorescent quantitative real-time polymerase chain reaction, which is a cutting-edge technology in genetic diagnosis, is used in different areas of molecularbiology.
M. I. Doronin, D. V. Mikhalishin, A. V. Sprygin, A. Mazloum, T. V. Zhbanova, K. N. Gruzdev, E. V. Chernyshova   +6 more
doaj   +1 more source

Specific Interaction between eEF1A and HIV RT Is Critical for HIV-1 Reverse Transcription and a Potential Anti-HIV Target.

PLoS Pathogens, 2015
Reverse transcription is the central defining feature of HIV-1 replication. We previously reported that the cellular eukaryotic elongation factor 1 (eEF1) complex associates with the HIV-1 reverse transcription complex (RTC) and the association is ...
Dongsheng Li, Ting Wei, Daniel J Rawle, Fangyun Qin, Rui Wang, Dinesh C Soares, Hongping Jin, Haran Sivakumaran, Min-Hsuan Lin, Kirsten Spann, Catherine M Abbott, David Harrich   +11 more
doaj   +1 more source

HIV-1 Uncoating and Nuclear Import Precede the Completion of Reverse Transcription in Cell Lines and in Primary Macrophages

Viruses, 2020
An assembly of capsid proteins (CA) form the mature viral core enclosing the HIV-1 ribonucleoprotein complex. Discrepant findings have been reported regarding the cellular sites and the extent of core disassembly (uncoating) in infected cells.
Ashwanth C. Francis, Mariana Marin, Mathew J. Prellberg, Kristina Palermino-Rowland, Gregory B. Melikyan   +4 more
doaj   +1 more source

Isothermal amplification using sequence-specific fluorescence detection of SARS coronavirus 2 and variants in nasal swabs

BioTechniques, 2022
Coronavirus disease 2019 is a public health challenge requiring rapid testing for the detection of infections and transmission. Nucleic acid amplification tests targeting SARS coronavirus 2 (CoV2) are used to detect CoV2 in clinical samples.
Les Jones, Hemant K Naikare, Yung-Yi C Mosley, Ralph A Tripp   +3 more
doaj   +1 more source

Full-Length cDNA Synthesis for Long-Distance RT-PCR of Large mRNA Transcripts

BioTechniques, 2003
Reverse transcription of mRNA often leads to the synthesis of partial, non-fulllength cDNAs. Methods to facilitate reverse transcription across RNA regions of secondary structure, as well as enzyme modifications to eliminate RNase H activities inherent ...
Phillip R. Hawkins, Pei Jin, Glenn K. Fu
doaj   +1 more source

SARS-CoV-2-Host Chimeric RNA-Sequencing Reads Do Not Necessarily Arise From Virus Integration Into the Host DNA

Frontiers in Microbiology, 2021
The human genome bears evidence of extensive invasion by retroviruses and other retroelements, as well as by diverse RNA and DNA viruses. High frequency of somatic integration of the RNA virus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2 ...
Anastasiya Kazachenka, George Kassiotis, George Kassiotis   +2 more
doaj   +1 more source

Intracytoplasmic maturation of the human immunodeficiency virus type 1 reverse transcription complexes determines their capacity to integrate into chromatin

Retrovirology, 2006
Background The early events of the HIV-1 life cycle include entry of the viral core into target cell, assembly of the reverse transcription complex (RTCs) performing reverse transcription, its transformation into integration-competent complexes called ...
Kashanchi Fatah, Altieri Maria, Berro Reem, Iordanskiy Sergey, Bukrinsky Michael   +4 more
doaj   +1 more source

Long Distance Reverse-Transcription PCR [PDF]

, 2003
Polymerase chain reaction (PCR) has been applied to the amplification of long DNA fragments from a variety of sources, including genomic, mitochondrial, and viral DNAs (1-5). We have adapted the concept of long PCR technology to reverse-transcription (RT) PCR (6).
Thiel, Volker, Herold, Jens, Siddell, Stuart G.   +2 more
openaire   +2 more sources

Quantification of HIV-1 DNA

Bio-Protocol, 2013
The reverse transcription (RT) reaction is a critical step in HIV-1 life cycle. It is very strongly regulated and the target of several restriction factors (TRIM5α, APOBECs, SAMHD1, etc.).
Ferdinand Roesch, Olivier Schwartz
doaj   +1 more source

Multiple recombinant events in human T-cell Leukemia virus Type 1: complete sequences of recombinant African strains

Emerging Microbes and Infections, 2020
Africa is the largest endemic area for HTLV-1, with many molecular genotypes. We previously demonstrated that some strains from North Africa (a-NA clade) originated from a recombinant event between Senegalese and West African strains.
Olivier Cassar, Alexandra Desrames, Ambroise Marçais, Olivier Gout, Graham P. Taylor, Olivier Hermine, Vicente Soriano, Carmen de Mendoza, Océane Dehan, Margot Le Mener, Philippe V. Afonso, Antoine Gessain   +11 more
doaj   +1 more source