Results 31 to 40 of about 23,635 (226)

Preliminary investigation of deoxyoligonucleotide binding to ribonuclease A using mass spectrometry: An attempt to develop a lab experience for undergraduates [version 1; referees: 2 approved]

F1000Research, 2018
Deoxyoligonucleotide binding to bovine pancreatic ribonuclease A (RNase A) was investigated using electrospray ionization ion-trap mass spectrometry (ESI-IT-MS). Deoxyoligonucleotides included CCCCC (dC5) and CCACC (dC2AC2).
Daniel D. Clark
doaj   +1 more source

Functional plasticity of antibacterial EndoU toxins. [PDF]

, 2018
Bacteria use several different secretion systems to deliver toxic EndoU ribonucleases into neighboring cells. Here, we present the first structure of a prokaryotic EndoU toxin in complex with its cognate immunity protein.
Eschenfeldt, William H, Goulding, Celia W, Hayes, Christopher S, Joachimiak, Andrzej, Jones, Allison M, Koskiniemi, Sanna, Low, David A, Michalska, Karolina, Nguyen, Josephine Y, Quan Nhan, Dinh, Stols, Lucy M, Willett, Julia LE   +11 more
core   +1 more source

Identification of the catalytic motif of the microbial ribosome inactivating cytotoxin colicin E3 [PDF]

, 2004
Colicin E3 is a cytotoxic ribonuclease that specifically cleaves 16S rRNA at the ribosomal A-site to abolish protein synthesis in sensitive Escherichia coli cells.
Bohm, Boon, Bowman, Carr, Cuchillo, Garinot-Schneider, Irie, Kerr, Kleanthous, Kleanthous, Makarov, Mosbahi, Ogawa, Ohno, Ohno, Ohno-Iwashita, Parret, Pommer, Rojas, Scholz, Schultz, Sidikaro, Smarda, Soelaiman, Steege, Tomita, Walker, Walker, Walker, Walker, Wallis   +30 more
core   +2 more sources

Affinity Labeling of Pancreatic Ribonuclease

Journal of Biological Chemistry, 1971
Abstract Pyridoxal phosphate can be firmly bound by reduction with sodium borohydride to many enzymes in which it occurs. Such treatment has been used to label specifically parts of peptide chains in the vicinity of the bound coenzyme. Pyridoxal phosphate may form specific complexes with many proteins for which it has no known coenzyme function. Bovine
G E, Means, R E, Feeney
openaire   +2 more sources

Ligand-Induced Variations in Structural and Dynamical Properties Within an Enzyme Superfamily

Frontiers in Molecular Biosciences, 2018
Enzyme catalysis is a complex process involving several steps along the reaction coordinates, including substrate recognition and binding, chemical transformation, and product release.
Chitra Narayanan, David N. Bernard, Khushboo Bafna, Donald Gagné, Pratul K. Agarwal, Pratul K. Agarwal, Nicolas Doucet, Nicolas Doucet   +7 more
doaj   +1 more source

Acid ribonuclease activity of hepatoma cells [PDF]

, 1962
Activities of intracellular RNase of the liver cytoplasm, normal liver cells exposed to 3'-Me-DAB and heaptoma cells, have been studied in correlation with the contents of RNA and DNA and morphologic changes of the cells with or without treating RNase ...
Hayashi, Kenji, Kimoto, Tetsuo, Monden, Hisashi   +2 more
core   +1 more source

Construction of Highly Stable Cytotoxic Nuclear-Directed Ribonucleases

Molecules, 2018
Ribonucleases are proteins whose use is promising in anticancer therapy. We have previously constructed different human pancreatic ribonuclease variants that are selectively cytotoxic for tumor cells by introducing a nuclear localization signal into ...
David Roura Padrosa, Jessica Castro, Alejandro Romero-Casañas, Marc Ribó, Maria Vilanova, Antoni Benito   +5 more
doaj   +1 more source

Folding of small disulfide-rich proteins : clarifying the puzzle [PDF]

, 2006
Premi a l'excel·lència investigadora. Àmbit de les Ciències Experimentals. 2008The process by which small proteins fold to their native conformations has been intensively studied over the last few decades.
Apuy, Arolas, Arolas, Arolas, Arolas, Baneyx, Bardwell, Cemazar, Cemazar, Chang, Chang, Chang, Chang, Chang, Chang, Chang, Chang, Chang, Chang, Chang, Chatrenet, Creighton, Creighton, Creighton, Creighton, Creighton, Daggett, Daly, Darby, Dobson, Ellgaard, Francesc X. Aviles, Fuller, Gehrmann, Goldberg, Goldenberg, Heitz, Hogg, Houry, Joan L. Arolas, Jui-Yoa Chang, Klein-Seetharaman, Kortemme, Kuhlman, Laity, Li, Lu, Matagne, Narayan, Narayan, Narayan, Narayan, Onuchic, Permyakov, Ross, Ruoppolo, Ruoppolo, Salamanca, Salvador Ventura, Scheraga, Shimotakahara, Shin, van den Berg, van den Berg, van Mierlo, van Mierlo, Venhudova, Wedemeyer, Weissman, Welker, Welker, Wu, Yang, Zavodszky   +73 more
core   +2 more sources

Vergleich dreier Ringversuche zur radioimmunologischen Thyrotropin-Bestimmung nach dem "Münchner Modell" [PDF]

, 1983
Peer ...
Habermann, J., König, A., Marschner, I., Scriba, Peter Christian, van Thiel, D., Wood, W. G.   +5 more
core   +2 more sources

NMR studies on structure and dynamics of the monomeric derivative of BS-RNase: new insights for 3D domain swapping. [PDF]

PLoS ONE, 2012
Three-dimensional domain swapping is a common phenomenon in pancreatic-like ribonucleases. In the aggregated state, these proteins acquire new biological functions, including selective cytotoxicity against tumour cells.
Roberta Spadaccini, Carmine Ercole, Maria A Gentile, Domenico Sanfelice, Rolf Boelens, Rainer Wechselberger, Gyula Batta, Andrea Bernini, Neri Niccolai, Delia Picone   +9 more
doaj   +1 more source