Results 41 to 50 of about 433,059 (284)

Cellular response to the ribonuclease injection; a morphologic and cytochemical study [PDF]

, 1960
By the repeated injection of RNase into mice the histological, cytochemical and electronmicroscope observations of several tissues and the quantitative estimation of DNA contents per cell in liver have been conducted.
Kimoto, Tetsuo, Matsuoka, Iwao, Seno, Satimaru, Utsumi, Kozo   +3 more
core   +1 more source

Molecular dynamics simulation of bovine pancreatic ribonuclease A-CpA and transition state-like complexes.

Journal of Physical Chemistry B, 2010
The mechanisms of enzymes are intimately connected with their overall structure and dynamics in solution. Experimentally, it is considerably challenging to provide detailed atomic level information about the conformational events that occur at different ...
Elena Formoso, J. M. Matxain, X. López, D. York   +3 more
semanticscholar   +1 more source

Folding, Quality Control, and Secretion of Pancreatic Ribonuclease in Live Cells*

Journal of Biological Chemistry, 2010
Although bovine pancreatic RNase is one of the best characterized proteins in respect to structure and in vitro refolding, little is known about its synthesis and maturation in the endoplasmic reticulum (ER) of live cells.
R. Geiger, M. Gautschi, F. Thor, Arnold Hayer, A. Helenius   +4 more
semanticscholar   +1 more source

The first crystal structure of human RNase6 reveals a novel substrate binding and cleavage site arrangement [PDF]

, 2016
Human RNase 6 is a cationic secreted protein that belongs to the RNase A superfamily. Its expression is induced in neutrophils and monocytes upon bacterial infection, suggesting a role in host defence.
Arranz Trullén, Javier, Blanco, JA., Boix i Borràs, Esther, Moussaoui, Mohammed., Nogués, MV., Prats, Guillem,, Pulido, Daniel.   +6 more
core   +2 more sources

NMR studies on structure and dynamics of the monomeric derivative of BS-RNase: new insights for 3D domain swapping. [PDF]

PLoS ONE, 2012
Three-dimensional domain swapping is a common phenomenon in pancreatic-like ribonucleases. In the aggregated state, these proteins acquire new biological functions, including selective cytotoxicity against tumour cells.
Roberta Spadaccini, Carmine Ercole, Maria A Gentile, Domenico Sanfelice, Rolf Boelens, Rainer Wechselberger, Gyula Batta, Andrea Bernini, Neri Niccolai, Delia Picone   +9 more
doaj   +1 more source

Acid ribonuclease activity of hepatoma cells [PDF]

, 1962
Activities of intracellular RNase of the liver cytoplasm, normal liver cells exposed to 3'-Me-DAB and heaptoma cells, have been studied in correlation with the contents of RNA and DNA and morphologic changes of the cells with or without treating RNase ...
Hayashi, Kenji, Kimoto, Tetsuo, Monden, Hisashi   +2 more
core   +1 more source

Identification of the catalytic motif of the microbial ribosome inactivating cytotoxin colicin E3 [PDF]

, 2004
Colicin E3 is a cytotoxic ribonuclease that specifically cleaves 16S rRNA at the ribosomal A-site to abolish protein synthesis in sensitive Escherichia coli cells.
Bohm, Boon, Bowman, Carr, Cuchillo, Garinot-Schneider, Irie, Kerr, Kleanthous, Kleanthous, Makarov, Mosbahi, Ogawa, Ohno, Ohno, Ohno-Iwashita, Parret, Pommer, Rojas, Scholz, Schultz, Sidikaro, Smarda, Soelaiman, Steege, Tomita, Walker, Walker, Walker, Walker, Wallis   +30 more
core   +2 more sources

Localization of Disulfide Bonds in Ribonuclease Using Low pH Trypsin and LC-ESI-QTOF-MS

CHIMIA
We evaluated a method to localize disulfide bonds in bovine pancreatic ribonuclease (RNase) by applying low pH trypsin protein digestion with a bottom-up LC-ESI-QTOF-MS approach.
Amélie Bornex, Saša M. Miladinović
doaj   +1 more source

Enhancement of the activity of the antimicrobial peptides HNP1 and LL-37 by bovine pancreatic ribonuclease A [version 3; peer review: 1 approved, 2 approved with reservations, 1 not approved]

F1000Research, 2023
Background: HNP1, LL-37, and HBD1 are antimicrobial against Escherichia coli ATCC 25922 at the standard inoculum but less active at higher inocula.   Methods: The virtual colony count (VCC) microbiological assay was adapted for high inocula and the ...
Bryan Ericksen
doaj  

A DIA‐MS‐based proteomics approach to find potential serum prognostic biomarkers in glioblastoma patients

Molecular Oncology, EarlyView.
A DIA‐MS‐based proteomics analysis of serum samples from GB patients and healthy controls showed that high levels of IL1R2 and low levels of CRTAC1 and HRG in serum are associated with poor survival outcomes for GB patients. These circulating proteins could serve as biomarkers for the prediction of outcome in patients with GB.
Anne Clavreul, François Guillonneau, Odile Blanchet, Hamza Lasla, Audrey Rousseau, Catherine Guette, Alice Boissard, Cécile Henry, Morgan Dhondt, Pascal Jézéquel, Philippe Menei, Jean‐Michel Lemée   +11 more
wiley   +1 more source