Results 31 to 40 of about 90,209 (297)

Programmed genome editing of the omega-1 ribonuclease of the blood fluke, Schistosoma mansoni

eLife, 2019
CRISPR/Cas9-based genome editing has yet to be reported in species of the Platyhelminthes. We tested this approach by targeting omega-1 (ω1) of Schistosoma mansoni as proof of principle.
Wannaporn Ittiprasert, Victoria H Mann, Shannon E Karinshak, Avril Coghlan, Gabriel Rinaldi, Geetha Sankaranarayanan, Apisit Chaidee, Toshihiko Tanno, Chutima Kumkhaek, Pannathee Prangtaworn, Margaret M Mentink-Kane, Christina J Cochran, Patrick Driguez, Nancy Holroyd, Alan Tracey, Rutchanee Rodpai, Bart Everts, Cornelis H Hokke, Karl F Hoffmann, Matthew Berriman, Paul J Brindley   +20 more
doaj   +1 more source

Novel Aspects of Polynucleotide Phosphorylase Function in Streptomyces

Antibiotics, 2018
Polynucleotide phosphorylase (PNPase) is a 3′–5′-exoribnuclease that is found in most bacteria and in some eukaryotic organelles. The enzyme plays a key role in RNA decay in these systems.
George H. Jones
doaj   +1 more source

Colletotrichum fructicola co-opts cytotoxic ribonucleases that antagonize host competitive microorganisms to promote infection

mBio
Phytopathogens secrete numerous molecules into the environment to establish a microbial niche and facilitate host infection. The phytopathogenic fungus Colletotrichum fructicola, which causes pear anthracnose, can colonize different plant tissues like ...
Chunhao Wang, Mengqing Han, Yanyan Min, Jiayi Hu, Yuemin Pan, Lili Huang, Jiajun Nie   +6 more
doaj   +1 more source

NaPi/SX-RNase segregates as a functional S-RNase and is induced under phosphate deficiency in Nicotiana alata

Biologia Plantarum, 2018
In plants, class III T2 RNases involves two groups of structurally similar proteins, but with different biological functions: S-RNases and non-S-RNases.
H. J. Rojas, C. Caspani, E. G. Escobar, R. Quiroga, A. Goldraij   +4 more
doaj   +1 more source

The Synthesis of Ribonuclease A

Journal of Biological Chemistry, 1971
A protected linear polypeptide of 124 amino acid residues with the sequence of bovine pancreatic ribonuclease A was synthesized by the solid phase method. The polypeptide was removed from the solid support and purified, and the four disulfide bonds were closed by air oxidation of the reduced form. The synthetic enzyme was fractionated by gel filtration
B, Gutte, R B, Merrifield
openaire   +2 more sources

Characterization of the biochemical properties of Campylobacter jejuni RNase III

Bioscience Reports, 2013
Campylobacter jejuni is a foodborne bacterial pathogen, which is now considered as a leading cause of human bacterial gastroenteritis. The information regarding ribonucleases in C.
Nabila Haddad, Margarida Saramago, Rute G. Matos, Hervé Prévost, Cecília M. Arraiano   +4 more
doaj   +1 more source

Regulation of RNase E during the UV stress response in the cyanobacterium Synechocystis sp. PCC 6803

mLife, 2023
Endoribonucleases govern the maturation and degradation of RNA and are indispensable in the posttranscriptional regulation of gene expression. A key endoribonuclease in Gram‐negative bacteria is RNase E.
Satoru Watanabe, Damir Stazic, Jens Georg, Shota Ohtake, Yutaka Sakamaki, Megumi Numakura, Munehiko Asayama, Taku Chibazakura, Annegret Wilde, Claudia Steglich, Wolfgang R. Hess   +10 more
doaj   +1 more source

RNase R, a New Virulence Determinant of Streptococcus pneumoniae

Microorganisms, 2022
Pneumococcal infections have increasingly high mortality rates despite the availability of vaccines and antibiotics. Therefore, the identification of new virulence determinants and the understanding of the molecular mechanisms behind pathogenesis have ...
Cátia Bárria, Dalila Mil-Homens, Sandra N. Pinto, Arsénio M. Fialho, Cecília M. Arraiano, Susana Domingues   +5 more
doaj   +1 more source

Structure and evolution of pancreatic ribonuclease [PDF]

, 1979
Dit proefschrift gaat over de structuur en de evolutie van het spijsverteringsenzym ribonuclease. In het eerste hoofdstuk wordt in het kort de stand van zaken beschreven in de eiwitchemie, waarvoor ribonuclease aI drie decennia dienst doet aIs ...
Lenstra, Johannes Arjen
core   +7 more sources

Evasion of Ribonuclease Inhibitor as a Determinant of Ribonuclease Cytotoxicity [PDF]

Current Pharmaceutical Biotechnology, 2008
Onconase (ONC) is an amphibian member of the bovine pancreatic ribonuclease (RNase A) superfamily that exhibits innate antitumoral activity. ONC has been granted both orphan-drug and fast-track status by the U.S. Food and Drug Administration for the treatment of malignant mesothelioma, and is poised to become the first chemotherapeutic agent based on a
Thomas J, Rutkoski, Ronald T, Raines
openaire   +2 more sources