Results 201 to 210 of about 61,956 (252)

Mechanistic basis for selective Csm6-2 activation by cyclic penta-adenylate in a type III CRISPR-Cas system. [PDF]

EMBO J
Shi R, Yang M, Liu Y, Gao H, Lin Z.
europepmc   +1 more source

Safety evaluation of the food enzyme ribonuclease P from the non-genetically modified <i>Penicillium citrinum</i> strain AE-RPE. [PDF]

EFSA J
EFSA Panel on Food Enzymes (FEZ), Zorn H, Barat Baviera JM, Bolognesi C, Catania F, Gadermaier G, Greiner R, Mayo B, Mortensen A, Roos YH, Solano MLM, Van Loveren H, Vernis L, Andryszkiewicz M, Cavanna D, Liu Y.   +15 more
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Calf thymus ribonuclease H IIa activity lacks ribonuclease H specificity

Experientia, 1990
Less purified fractions of ribonuclease H IIa activity of calf thymus display divalent cation-dependent ribonuclease H activity and divalent cation-independent ribonuclease activity. Because the ratio of the two enzyme activities does not change during successive chromatographic procedures, we suggest that ribonuclease H IIa activity is indeed able to ...
H, Vonwirth, P, Frank, W, Büsen
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Ribonuclease H

1995
Abstract The ribonuclease H (RNase H) is an essential activity associated with HIV reverse transcriptase (RT). RNase H cleaves the RNA in hybrid RNA-DNA intermediates during minus-strand DNA synthesis initiated from the tRNALys, 3 primer.
Z Hostomska, Z Hostomsky
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A capillary electrophoretic assay for ribonuclease H activity

Analytical Biochemistry, 2004
A capillary electrophoretic assay was developed to measure the ribonuclease (RNase) H activity of human immunodeficiency virus (HIV) type 1 reverse transcriptase. Cleavage of a fluorescein-labeled RNA-DNA heteroduplex was monitored by capillary electrophoresis.
King C, Chan, Scott R, Budihas, Stuart F J, Le Grice, Michael A, Parniak, Robert J, Crouch, Sergei A, Gaidamakov, Haleem J, Isaaq, Antony, Wamiru, James B, McMahon, John A, Beutler   +9 more
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Functions and Structures of Ribonuclease H Enzymes

1995
Ribonuclease H* (RNase H, EC 3.1.26.4) is an endonuclease that specifically hydrolyzes an RNA hybridized to a complementary DNA to produce an oli-goribonucleotide with 5′-phosphate and 3′-hydroxyl groups (Fig. 1). It requires divalent cations, such as Mg2+ and Mn2+, for activity.
S, Kanaya, M, Ikehara
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Ribonuclease H activity in developing rat brain

Life Sciences, 1977
Abstract A ribonucleolytic enzyme (RNase H) which degrades the RNA strand of a RNA-DNA double stranded hybrid has been extracted from rat brain and characterized. RNase H activity in the cerebella increased up to around 6th day after birth and then decreased in adult rat cerebella, just as DNA polymerase and DNA ligase.
Y, Sawai, Y, Sawasaki, K, Tsukada
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Thermal and mechanical multistate folding of ribonuclease H

The Journal of Chemical Physics, 2009
Two different classes of experimental techniques exist by which protein folding mechanisms are ascertained. The first class, of which circular dichroism is an example, probes thermally-induced folding. The second class, which includes atomic force microscopy and optical tweezers, measures mechanically-induced folding. In this article, we investigate if
Terry J, Schmitt, Jonathan E, Clark, Thomas A, Knotts   +2 more
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Ribonuclease H evolution in retrotransposable elements

Cytogenetic and Genome Research, 2005
Eukaryotic and prokaryotic genomes encode either Type I or Type II Ribonuclease H (RNH) which is important for processing RNA primers that prime DNA replication in almost all organisms. This review highlights the important role that Type I RNH plays in the life cycle of many retroelements, and its utility in tracing early events in retroelement ...
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Ribonuclease H activity in cultured plant cells

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1978
Ribonuclease H (RNAase H) was extracted from cultured plant cells, strain GD-2 and characterized. RNAase H activity in logarithmical growing cells is much higher than that of stationary cells, and the response of RNAase H activity was very similar to that of DNA polymerase after culture.
Y, Sawai, N, Sugano, K, Tsukada
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