Molecular recognition of pre-tRNA by Arabidopsis protein-only Ribonuclease P. [PDF]
RNA, 2017 Protein-only ribonuclease P (PRORP) is an enzyme responsible for catalyzing the 5′ end maturation of precursor transfer ribonucleic acids (pre-tRNAs) encoded by various cellular compartments in many eukaryotes.
Klemm BP +9 more
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Structural insight into the human mitochondrial tRNA purine N1-methyltransferase and ribonuclease P complexes. [PDF]
J Biol Chem, 2018 Mitochondrial tRNAs are transcribed as long polycistronic transcripts of precursor tRNAs and undergo posttranscriptional modifications such as endonucleolytic processing and methylation required for their correct structure and function.
Oerum S +12 more
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Distributive enzyme binding controlled by local RNA context results in 3' to 5' directional processing of dicistronic tRNA precursors by Escherichia coli ribonuclease P. [PDF]
Nucleic Acids Res, 2019 RNA processing by ribonucleases and RNA modifying enzymes often involves sequential reactions of the same enzyme on a single precursor transcript. In Escherichia coli, processing of polycistronic tRNA precursors involves separation into individual pre ...
Zhao J, Harris ME.
europepmc +2 more sources
Differential substrate recognition by isozymes of plant protein-only Ribonuclease P. [PDF]
RNA, 2016 Ribonuclease P (RNase P) catalyzes the cleavage of leader sequences from precursor tRNA (pre-tRNA). Typically, these enzymes are ribonucleic protein complexes that are found in all domains of life. However, a new class of RNase P has been discovered that
Howard MJ +6 more
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Loss of the mitochondrial protein-only ribonuclease P complex causes aberrant tRNA processing and lethality in Drosophila. [PDF]
Nucleic Acids Res, 2016 Proteins encoded by mitochondrial DNA are translated using mitochondrially encoded tRNAs and rRNAs. As with nuclear encoded tRNAs, mitochondrial tRNAs must be processed to become fully functional.
Sen A +5 more
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Safety evaluation of the food enzyme ribonuclease P from the non‐genetically modified Penicillium citrinum strain AE‐RPE [PDF]
EFSA JournalThe food enzyme ribonuclease P (EC 3.1.26.5) is produced with the non‐genetically modified Penicillium citrinum strain AE‐RPE by Amano Enzyme Inc. It was considered free from viable cells of the production organism.
EFSA Panel on Food Enzymes (FEZ) +15 more
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The contribution of the C5 protein subunit of Escherichia coli ribonuclease P to specificity for precursor tRNA is modulated by proximal 5' leader sequences. [PDF]
RNA, 2017 Recognition of RNA by RNA processing enzymes and RNA binding proteins often involves cooperation between multiple subunits. However, the interdependent contributions of RNA and protein subunits to molecular recognition by ribonucleoproteins are ...
Niland CN +3 more
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The Ribonuclease P Database [PDF]
Nucleic Acids Research, 1996 Ribonuclease P is responsible for the 5'-maturation of tRNA precursors. Ribonuclease P is a ribonucleoprotein, and in bacteria (and some Archaea) the RNA subunit alone is catalytically active in vitro, i.e. it is a ribozyme. The Ribonuclease P Database is a compilation of ribonuclease P sequences, sequence alignments, secondary structures, three ...
James W. Brown
openaire +9 more sources
Mechanistic Studies Reveal Similar Catalytic Strategies for Phosphodiester Bond Hydrolysis by Protein-only and RNA-dependent Ribonuclease P. [PDF]
J Biol Chem, 2015 Background: A protein-only ribonuclease P (PRORP) has been recently discovered. Results: PRORP activity has a single ionization (pKa ∼ 8.7) important for catalysis and a cooperative dependence on Mg2+ (nH = 2). Conclusion: PRORP uses catalytic strategies
Howard MJ, Klemm BP, Fierke CA.
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Inhibition of hepatitis B virus gene expression and replication by ribonuclease P. [PDF]
Mol Ther, 2013 Nucleic acid-based gene interfering approaches, such as those mediated by RNA interference and RNase P-associated external guide sequence (EGS), have emerged as promising antiviral strategies.
Xia C +8 more
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