Calibrated ribosome profiling assesses the dynamics of ribosomal flux on transcripts [PDF]
Nature CommunicationsRibosome profiling, which is based on deep sequencing of ribosome footprints, has served as a powerful tool for elucidating the regulatory mechanism of protein synthesis.
Kotaro Tomuro +9 more
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Active Ribosome Profiling with RiboLace [PDF]
Cell Reports, 2018 Summary: Ribosome profiling, or Ribo-seq, is based on large-scale sequencing of RNA fragments protected from nuclease digestion by ribosomes. Thanks to its unique ability to provide positional information about ribosomes flowing along transcripts, this ...
Massimiliano Clamer +15 more
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Advances in ribosome profiling technologies. [PDF]
Biochem Soc TransRibosome profiling (or Ribo-seq) has emerged as a powerful approach for revealing the regulatory mechanisms of protein synthesis, on the basis of deep sequencing of ribosome footprints. Recent innovations in Ribo-seq technologies have significantly enhanced their sensitivity, specificity, and resolution.
Tomuro K, Iwasaki S.
europepmc +3 more sources
Protocol for Ribosome Profiling in Bacteria [PDF]
Bio-Protocol, 2019 Ribosome profiling provides information on the position of ribosomes on mRNA on a genomic scale. Although this information is often used to detect changes in gene expression under different conditions, it also has great potential for yielding insight ...
Fuad Mohammad, Allen Buskirk
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riboWaltz: Optimization of ribosome P-site positioning in ribosome profiling data. [PDF]
PLoS Computational Biology, 2018 Ribosome profiling is a powerful technique used to study translation at the genome-wide level, generating unique information concerning ribosome positions along RNAs.
Fabio Lauria +5 more
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Ribosome profiling: a powerful tool in oncological research [PDF]
Biomarker ResearchNeoplastic cells need to adapt their gene expression pattern to survive in an ever-changing or unfavorable tumor microenvironment. Protein synthesis (or mRNA translation), an essential part of gene expression, is dysregulated in cancer.
Dan Su +9 more
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Ribosome profiling enhances understanding of mycobacterial translation
Frontiers in Microbiology, 2022 A recent addition to the -omics toolkit, ribosome profiling, enables researchers to gain insight into the process and regulation of translation by mapping fragments of mRNA protected from nuclease digestion by ribosome binding. In this review, we discuss
Elizabeth B. Sawyer, Teresa Cortes
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Common cell lysis procedures distort ribosome profiling analyses of gene expression [PDF]
Genome BiologyRibosome profiling is a powerful technique used to study gene expression on a transcriptome-wide scale. It involves sequencing of mRNA fragments protected by ribosomes from ribonuclease digestion. The initial steps commonly involve cell lysis followed by
Aoife O’Connell +6 more
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Ribosome profiling reveals downregulation of UMP biosynthesis as the major early response to phage infection [PDF]
Microbiology SpectrumBacteria have evolved diverse defense mechanisms to counter bacteriophage attacks. Genetic programs activated upon infection characterize phage–host molecular interactions and ultimately determine the outcome of the infection.
Patrick B. F. O’Connor +5 more
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Identification and analysis of ribosome-associated lncRNAs using ribosome profiling data [PDF]
BMC Genomics, 2018 Background Although the number of discovered long non-coding RNAs (lncRNAs) has increased dramatically, their biological roles have not been established.
Chao Zeng +2 more
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