Results 11 to 20 of about 43,196 (233)
Identifying ribosome heterogeneity using ribosome profiling
Nucleic Acids Research, 2022 Abstract Recent studies have revealed multiple mechanisms that can lead to heterogeneity in ribosomal composition. This heterogeneity can lead to preferential translation of specific panels of mRNAs, and is defined in large part by the ribosomal protein (RP) content, amongst other things.
Ferhat Alkan +6 more
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A tool for analyzing and visualizing ribo-seq data at the isoform level
BMC Bioinformatics, 2021 Background Translational regulation is one important aspect of gene expression regulation. Dysregulation of translation results in abnormal cell physiology and leads to diseases.
Wei-Sheng Wu +5 more
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Genome-wide Survey of Ribosome Collision
Cell Reports, 2020 Summary: Ribosome movement is not always smooth and is rather often impeded. For ribosome pauses, fundamental issues remain to be addressed, including where ribosomes pause on mRNAs, what kind of RNA/amino acid sequence causes this pause, and the ...
Peixun Han +10 more
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Nature Communications, 2021 Ribosome profiling has become the gold standard to analyze mRNA translation dynamics, and the translation inhibitor cycloheximide (CHX) is often used in its application.
Puneet Sharma +3 more
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Profiling of Mycoplasma gallisepticum Ribosomes [PDF]
Acta Naturae, 2015 The development of high-throughput technologies is increasingly resulting in identification of numerous cases of low correlation between mRNA and the protein level in cells. These controversial observations were made on various bacteria, such as E. coli, Desulfovibrio vulgaris, and Lactococcus lactis.
FISUNOV G.Y. +4 more
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F1000Research, 2020 Ribosome profiling provides the opportunity to evaluate translation kinetics at codon level resolution. Here, we describe ribosome profiling data, generated from two HEK293T cell lines.
Aikaterini Alexaki +11 more
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Quantitative Modeling of Protein Synthesis Using Ribosome Profiling Data
Frontiers in Molecular Biosciences, 2021 Quantitative prediction on protein synthesis requires accurate translation initiation and codon translation rates. Ribosome profiling data, which provide steady-state distribution of relative ribosome occupancies along a transcript, can be used to ...
Vandana Yadav +3 more
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PLoS Computational Biology, 2020 Ribosome profiling, an application of nucleic acid sequencing for monitoring ribosome activity, has revolutionized our understanding of protein translation dynamics.
Jordan A Berg +7 more
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Ribonuclease selection for ribosome profiling [PDF]
Nucleic Acids Research, 2016 Ribosome profiling has emerged as a powerful method to assess global gene translation, but methodological and analytical challenges often lead to inconsistencies across labs and model organisms. A critical issue in ribosome profiling is nuclease treatment of ribosome-mRNA complexes, as it is important to ensure both stability of ribosomal particles and
Gerashchenko, Maxim, Gladyshev, Vadim
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Ribosome Profiling of
mSystems, 2018 Cyanobacteria experience both rapid and periodic fluctuations in light and inorganic carbon (Ci) and have evolved regulatory mechanisms to respond to these, including extensive posttranscriptional gene regulation. We report the first genome-wide ribosome
Jan Karlsen +4 more
doaj +3 more sources