Results 21 to 30 of about 43,196 (233)
Development of a tissue-specific ribosome profiling approach in Drosophila enables genome-wide evaluation of translational adaptations. [PDF]
PLoS Genetics, 2017 Recent advances in next-generation sequencing approaches have revolutionized our understanding of transcriptional expression in diverse systems. However, measurements of transcription do not necessarily reflect gene translation, the process of ultimate ...
Xun Chen, Dion Dickman
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eLife, 2014 During translation elongation, the ribosome ratchets along its mRNA template, incorporating each new amino acid and translocating from one codon to the next. The elongation cycle requires dramatic structural rearrangements of the ribosome.
Liana F Lareau +3 more
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Frontiers in Molecular Biosciences, 2022 Our understanding of mRNA translation and its regulation has been transformed by the development of ribosome profiling. This approach relies upon RNase footprinting of translating ribosomes in a precise manner to generate an accurate snapshot of ribosome
Katerina Douka +7 more
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RiboProP: a probabilistic ribosome positioning algorithm for ribosome profiling [PDF]
Bioinformatics, 2018 Abstract Motivation Ribosome profiling has been widely used to study translation in a genome-wide fashion. It requires deep sequencing of ribosome protected mRNA fragments followed by mapping of fragments to the reference genome. For applications such as identification of ribosome pausing sites, it is
Dengke Zhao +3 more
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Genome-wide Translation Profiling by Ribosome-Bound tRNA Capture
Cell Reports, 2018 Summary: In the ribosome complex, tRNA is a critical element of mRNA translation. A rich repertoire of cell regulation is hypothesized to occur during the recruitment of specific tRNAs in polypeptide formation.
Chien-Wen Chen, Motomasa Tanaka
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In Vitro Analysis of Stalled Ribosomes using Puromycin Incorporation
Bio-Protocol, 2023 Ribosome footprint profiling has demonstrated that ribosomes can be slowed or stalled on select mRNAs, often due to the presence of rare codons, stalling motifs, or via a ribosome-binding protein (e.g., FMRP).
MaKenzie Scarpitti, Michael Kearse
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Ribosome Profiling: Global Views of Translation [PDF]
Cold Spring Harbor Perspectives in Biology, 2018 The translation of messenger RNA (mRNA) into protein and the folding of the resulting protein into an active form are prerequisites for virtually every cellular process and represent the single largest investment of energy by cells. Ribosome profiling-based approaches have revolutionized our ability to monitor every step of protein synthesis in vivo ...
Nicholas T, Ingolia +2 more
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High-Precision Analysis of Translational Pausing by Ribosome Profiling in Bacteria Lacking EFP
Cell Reports, 2015 Ribosome profiling is a powerful method for globally assessing the activity of ribosomes in a cell. Despite its application in many organisms, ribosome profiling studies in bacteria have struggled to obtain the resolution necessary to precisely define ...
Christopher J. Woolstenhulme +3 more
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Protocol for Disome Profiling to Survey Ribosome Collision in Humans and Zebrafish
STAR Protocols, 2020 Summary: Ribosomes often encounter obstacles during translation elongation and thus collide with each other. Disome profiling, an optimized ribosome profiling method, specifically sequences the long ribosome footprints generated from collided ribosomes ...
Mari Mito +2 more
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Normalized Ribo-Seq for Quantifying Absolute Global and Specific Changes in Translation
Bio-Protocol, 2022 Ribosome profiling (Ribo-Seq) is a highly sensitive method to quantify ribosome occupancies along individual mRNAs on a genome-wide scale. Hereby, ribosome-protected fragments (= footprints) are generated by nuclease digestion, isolated, and sequenced ...
Katharina Hoerth +2 more
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